The development of ISSR-derived SCAR markers around the SEASONAL FLOWERING LOCUS (SFL) in Fragaria vesca

Albani, Maria C.; Battey, Nicholas H.; Wilkinson, M. J.

doi:10.1007/s00122-004-1654-4

Cited by 79 publications

(48 citation statements)

References 27 publications

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“…chiloensis sex chromosome is correct is our finding of the SCAR2 marker at the top of VI-A; this marker has been shown to cosegregate with the seasonal flowering locus (Albani et al 2004), which has been mapped to the top of LG 6 in the diploid FV3FN map (Sargent et al 2004).…”

Section: Sex Expressionmentioning

confidence: 85%

Comparative Genetic Mapping Points to Different Sex Chromosomes in Sibling Species of Wild Strawberry (Fragaria)

2010

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Separate sexes have evolved repeatedly from hermaphroditic ancestors in flowering plants, and thus select taxa can provide unparalleled insight into the evolutionary dynamics of sex chromosomes that are thought to be shared by plants and animals alike. Here we ask whether two octoploid sibling species of wild strawberry-one almost exclusively dioecious (males and females), Fragaria chiloensis, and one subdioecious (males, females, and hermaphrodites), F. virginiana-share the same sex-determining chromosome. We created a genetic map of the sex chromosome and its homeologs in F. chiloensis and assessed macrosynteny between it and published maps of the proto-sex chromosome of F. virginiana and the homeologous autosome of hermaphroditic diploid species. Segregation of male and female function in our F. chiloensis mapping population confirmed that linkage and dominance relations are similar to those in F. virginiana. However, identification of the molecular markers most tightly linked to the sexdetermining locus in the two octoploid species shows that, in both, this region maps to homeologues of chromosome 6 in diploid congeners, but is located at opposite ends of their respective chromosomes.

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Section: Sex Expressionmentioning

confidence: 85%

Comparative Genetic Mapping Points to Different Sex Chromosomes in Sibling Species of Wild Strawberry (Fragaria)

2010

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“…Early genetic studies have shown that different single genes, SEASONAL FLOWERING LOCUS (SFL) and RUNNERING LOCUS (RL), regulate seasonal flowering and runner formation in strawberry, and recessive alleles of these loci cause perpetual (continuous) flowering and runner-less phenotypes, respectively (Brown and Wareing, 1965;Albani et al, 2004). RL has not been identified.…”

Section: Introductionmentioning

confidence: 99%

TheFragaria vescaHomolog of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 Represses Flowering and Promotes Vegetative Growth

et al. 2013

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In the annual long-day plant Arabidopsis thaliana, SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) integrates endogenous and environmental signals to promote flowering. We analyzed the function and regulation of the SOC1 homolog (Fragaria vesca [Fv] SOC1) in the perennial short-day plant woodland strawberry (Fragaria vesca). We found that Fv SOC1 overexpression represses flower initiation under inductive short days, whereas its silencing causes continuous flowering in both short days and noninductive long days, similar to mutants in the floral repressor Fv TERMINAL FLOWER1 (Fv TFL1). Molecular analysis of these transgenic lines revealed that Fv SOC1 activates Fv TFL1 in the shoot apex, leading to the repression of flowering in strawberry. In parallel, Fv SOC1 regulates the differentiation of axillary buds to runners or axillary leaf rosettes, probably through the activation of gibberellin biosynthetic genes. We also demonstrated that Fv SOC1 is regulated by photoperiod and Fv FLOWERING LOCUS T1, suggesting that it plays a central role in the photoperiodic control of both generative and vegetative growth in strawberry. In conclusion, we propose that Fv SOC1 is a signaling hub that regulates yearly cycles of vegetative and generative development through separate genetic pathways.

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“…Because of these reasons, ISSR are being used for population authentication and population molecular ecology studies. [8][9][10][11][12][13][14][15][16] The present study was undertaken with the objective of establishing specific molecular markers using ISSR for authenticating eight wild D. officinale populations. …”

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confidence: 99%

Intersimple Sequence Repeats (ISSR) Molecular Fingerprinting Markers for Authenticating Populations of Dendrobium officinale KIMURA et MIGO

Shen

Ding

Liu

et al. 2006

Biological & Pharmaceutical Bulletin

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(previous name: Dendrobium candidum WALL. ex LINDL. 2)) is an endangered species in China and ranked "the first of the nine Chinese fairy herbs." The stems of D. officinale have been used as a traditional Chinese tonic medicine called Tiepi Fengdou 3) for hundreds of years. It can benefit human health in many aspects, such as nourishing yin and clearing away unhealthy heat, benefiting the stomach, promoting the production of body fluid, resisting cancer and prolonging life 1,3) This treasured herb is in severe shortage, making it expensive; the highest grade costs ¥12000-30000 per kg. 4)In our previous studies, rDNA ITS regions of D. officinale and other four adulterant species were sequenced. A pair of allele-specific diagnostic primers was designed to authenticate D. officinale from the other species based on rDNA ITS sequences of D. officinale and the other 37 species of Dendrobium.5,6) Recently, we have focused on the authentication of D. officinale populations using DNA molecular markers, which will lay the foundation for the selection of high-quality population resources.Intersimple sequence repeats (ISSR) have become a good DNA molecular marker for research on populations of the same species, a technique established based on PCR by Zietkiewicz et al. in 1994. 7) The main advantages of ISSR are: no need for DNA sequence information prior to amplification, low cost, simple operation, high stability, and abundance of genomic information. Because of these reasons, ISSR are being used for population authentication and population molecular ecology studies. [8][9][10][11][12][13][14][15][16] The present study was undertaken with the objective of establishing specific molecular markers using ISSR for authenticating eight wild D. officinale populations. MATERIALS AND METHODS Plant MaterialsAll materials used in this study were collected in China. Voucher samples were identified by Dr. Xiaoyu Ding and preserved by means of tissue culture in the College of Life Sciences, Nanjing Normal University. Wherever possible, three to five individuals were sampled from each population. The details are shown in Table 1.DNA Extraction Complete genomic DNA samples were extracted from fresh leaves using Dneasy Plant Mini Kits (QIAGEN), the concentrations of the DNA samples were measured spectrophotometrically, and each sample was diluted to 20 ng ml Ϫ1 with TE buffer for PCR amplification. Selection of Primers To select suitable primers for the study of populations of D. officinale, 76 ISSR primers, which were all purchased from Sangon Co., Ltd., China, were screened using two DNA samples. From the preliminary screening, 32 primers that could amplify visible bands were selected for further examination. Eventually, 10 ISSR primers that produced clear and reproducible bands were selected for the amplification of all DNA samples (Table 2).ISSR Profiling ISSR amplifications were carried out in a 25 ml volume containing Tris-HCl 10 mM, KCl 10 mM, (NH 4 ) 2 SO 4 8 mM (pH 9.0), MgCl 2 1.2-1.8 mM, Taq DNA polymerase1.5 U, dNTP 80 mM, pri...

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The development of ISSR-derived SCAR markers around the SEASONAL FLOWERING LOCUS (SFL) in Fragaria vesca

Cited by 79 publications

References 27 publications

Comparative Genetic Mapping Points to Different Sex Chromosomes in Sibling Species of Wild Strawberry (Fragaria)

Comparative Genetic Mapping Points to Different Sex Chromosomes in Sibling Species of Wild Strawberry (Fragaria)

TheFragaria vescaHomolog of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 Represses Flowering and Promotes Vegetative Growth

Intersimple Sequence Repeats (ISSR) Molecular Fingerprinting Markers for Authenticating Populations of Dendrobium officinale KIMURA et MIGO

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