The development of immunofluorescence and the immunopathology of the skin

Beutner, Ernst H.

doi:10.1046/j.1365-4362.2003.01202.x

Cited by 7 publications

(4 citation statements)

References 37 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The archival biopsies were IRB exempt due to the lack of patient identifiers. In both dermatopathology laboratories, each biopsy also was sent for direct immunofluorescence as previous described [3], for correlation with the H&E diagnoses.…”

Section: Subjects Of Studymentioning

confidence: 99%

See 1 more Smart Citation

Cyclo-oxygenase 2 is present in the majority of lesional skin from patients with autoinmune blistering diseases

Velez¹,

Isaza²,

Howard³

2013

Our Dermatol Online

View full text Add to dashboard Cite

Introduction: The in situ immune response within skin biopsies from patients affected by autoimmune skin blistering diseases (ABDs) is not well characterized. Aim: Based on the fact that the ABD immune response is considered an adaptive immune response, both an innate immune response and inflammation would be expected in these diseases. Our investigation investigates the presence of cyclo-oxygenase-2 (COX-2), since this enzyme is commonly involved in innate immune responses. Methods: We utilized immunohistochemistry (IHC) to evaluate the presence of COX-2 in lesional skin biopsies of patients affected by ABDs. We tested 30 patients with endemic pemphigus foliaceus (EPF), 15 controls from the endemic area, and 15 biopsies from healthy controls from the USA. We also tested archival biopsies from patients with selected ABDs, including 20 patients with bullous pemphigoid, 20 with pemphigus vulgaris, 8 with pemphigus foliaceus and 12 with dermatitis herpetiformis. Results: Most ABD biopsies stained positive for COX-2 in the lesional blister and/or the dermal inflammatory infiltrate, accentuated in the upper neurovascular plexus. In BP and EPF, the COX-2 staining was also seen in the sweat glands. All controls were negative. Conclusions: We document that COX-2 is expressed in lesional skin of patients with ABDs.

show abstract

Section: Subjects Of Studymentioning

confidence: 99%

“…Steroids represent a commonly utilized therapy, because many autoimmune disorders are B-lymphocyte mediated processes depositing autoantibodies and complement deposits in the skin. Well documented correlations exist between titers of autoantibodies and the clinical severity of the diseases [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Cyclo-oxygenase 2 is present in the majority of lesional skin from patients with autoinmune blistering diseases

Velez¹,

Isaza²,

Howard³

2013

Our Dermatol Online

View full text Add to dashboard Cite

show abstract

“…Epiluminescence and confocal microscopy can be both used to read the results of DIF. 2 Three distinct forms of fluorescence should be listed in the readings of IF assays: specific and nonspecific fluorescence and autofluorescence. Specific fluorescence is due to a reaction between the substrate and the protein labeled with fluorochrome (antigenantibody reaction).…”

Section: Introductionmentioning

confidence: 99%

Imunofluorescência direta e indireta

Aoki

Sousa

Fukumori

et al. 2010

An. Bras. Dermatol.

View full text Add to dashboard Cite

Abstract:Immunofluorescence is a valuable auxiliary diagnostic tool for autoimmune bullous diseases and inflammatory disorders, since their clinical and histopathologic findings may be inconclusive. It is a feasible laboratory method that requires experienced technicians and detects in situ and circulating immune deposits that may be involved in the pathogenesis of such skin diseases. Keywords: Autoimmunity; Basement membrane; Fluorescent antibody technique; Pemphigus Resumo: A imunofluorescência é um valioso instrumento auxiliar no diagnóstico das dermatoses bolhosas autoimunes e desordens inflamatórias, uma vez que seus achados clínicos e histopatológicos podem não ser determinantes. Consiste em um método laboratorial factível, que requer profissionais técnicos experientes, e detecta imunocomplexos in situ e/ou circulantes, que podem estar envolvidos na patogênese de tais enfermidades cutâneas.

show abstract

“…The procedure for DIF has been explained in Figure 7 . [ 25 ] DIF is diagnostic in pemphigus, pemphigoid, gestational pemphigoid, dermatitis herpatiformis, linear IgA bullous dermatosis, and epidermolysis bullosa acquisita; and the findings of these lesions by DIF technique has been listed in Table 5 .…”

Section: Immunofluorescent Techniquesmentioning

confidence: 99%

Diagnostic procedures for autoimmune vesiculobullous diseases: A review

Rastogi¹,

Sharma

Misra

et al. 2014

J Oral Maxillofac Pathol

View full text Add to dashboard Cite

Oral soft tissues are affected by numerous pathologic conditions of variable etiology and hence their appropriate management relies on their accurate diagnosis. Clinical identification of intact vesicle and bulla in the oral cavity is really a challenge due to the regular irritation and the friable nature of oral mucosa. Rupture of these lesions leads to erosions or ulcerations on the surface, hence making the diagnosis of vesiculobullous (VB) lesions is even more difficult due to the fact that the differential diagnosis along with VB lesions will also include ulcerative, immunological-mediated diseases, and neoplasms and systemic diseases. Hence, knowledge of the clinical presentation of these disorders and the relevant diagnostic procedures is important not just for dermatologists, but also for general practitioners and dentists. In this article, the various procedures have been explained that can be used for the diagnostic purpose of VB lesions.

show abstract

The development of immunofluorescence and the immunopathology of the skin

Cited by 7 publications

References 37 publications

Cyclo-oxygenase 2 is present in the majority of lesional skin from patients with autoinmune blistering diseases

Cyclo-oxygenase 2 is present in the majority of lesional skin from patients with autoinmune blistering diseases

Imunofluorescência direta e indireta

Diagnostic procedures for autoimmune vesiculobullous diseases: A review

Contact Info

Product

Resources

About