The Development of Immunoassays to Identify and Quantify Species Source of Gum Arabic

Ireland, H. Elyse; Clutterbuck, Andy; Cloquet, Jean-Phillipe; Thurston, Milo; Williams, Peter; Cronk, Quentin; Dewey, F. M.; Williams, John

doi:10.1021/jf048864h

Cited by 7 publications

(3 citation statements)

References 13 publications

(25 reference statements)

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“…All the unique sequences were used to search for microsatellite makers using Msatfinder [31] with a repeat threshold of eight di-nucleotide repeats or five tri-, tetra-, penta-, or hexa- nucleotide repeats. The presence of at least 50-bp sequence on both sides of the microsatellite repeats were considered sufficient for primer design [32,33].…”

Section: Methodsmentioning

confidence: 99%

Generation of genome-scale gene-associated SNPs in catfish for the construction of a high-density SNP array

et al. 2011

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BackgroundSingle nucleotide polymorphisms (SNPs) have become the marker of choice for genome-wide association studies. In order to provide the best genome coverage for the analysis of performance and production traits, a large number of relatively evenly distributed SNPs are needed. Gene-associated SNPs may fulfill these requirements of large numbers and genome wide distribution. In addition, gene-associated SNPs could themselves be causative SNPs for traits. The objective of this project was to identify large numbers of gene-associated SNPs using high-throughput next generation sequencing.ResultsTranscriptome sequencing was conducted for channel catfish and blue catfish using Illumina next generation sequencing technology. Approximately 220 million reads (15.6 Gb) for channel catfish and 280 million reads (19.6 Gb) for blue catfish were obtained by sequencing gene transcripts derived from various tissues of multiple individuals from a diverse genetic background. A total of over 35 billion base pairs of expressed short read sequences were generated. Over two million putative SNPs were identified from channel catfish and almost 2.5 million putative SNPs were identified from blue catfish. Of these putative SNPs, a set of filtered SNPs were identified including 342,104 intra-specific SNPs for channel catfish, 366,269 intra-specific SNPs for blue catfish, and 420,727 inter-specific SNPs between channel catfish and blue catfish. These filtered SNPs are distributed within 16,562 unique genes in channel catfish and 17,423 unique genes in blue catfish.ConclusionsFor aquaculture species, transcriptome analysis of pooled RNA samples from multiple individuals using Illumina sequencing technology is both technically efficient and cost-effective for generating expressed sequences. Such an approach is most effective when coupled to existing EST resources generated using traditional sequencing approaches because the reference ESTs facilitate effective assembly of the expressed short reads. When multiple individuals with different genetic backgrounds are used, RNA-Seq is very effective for the identification of SNPs. The SNPs identified in this report will provide a much needed resource for genetic studies in catfish and will contribute to the development of a high-density SNP array. Validation and testing of these SNPs using SNP arrays will form the material basis for genome association studies and whole genome-based selection in catfish.

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Section: Methodsmentioning

confidence: 99%

Generation of genome-scale gene-associated SNPs in catfish for the construction of a high-density SNP array

et al. 2011

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“…Enzyme-linked immunosorbent assays (ELISAs), which are relatively simple to use, rapid, and cost effective, also have the potential to be used for gums (Haines & Patel, 1997;Ireland et al, 2004;Pickles et al, 2007). This technique utilises antibodies that have the ability to detect specific epitopes on target molecules and we have previously utilised this specificity to distinguish between gums from different sources (Dewey et al, 1997;Thurston et al, 1998;Ireland et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…This technique utilises antibodies that have the ability to detect specific epitopes on target molecules and we have previously utilised this specificity to distinguish between gums from different sources (Dewey et al, 1997;Thurston et al, 1998;Ireland et al, 2004). The specific nature of the antibody-antigen interaction minimises the interference from matrix components and makes immunoassays ideally suited to food analysis .…”

Section: Introductionmentioning

confidence: 99%

Detection of Konjac glucomannan by immunoassay

Hurley

Qin

Ireland

et al. 2010

International Journal of Food Science &Amp; Technology

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Konjac glucomannan is a hydrocolloid that has been used in food applications. The European ban on the use of Konjac glucomannan means that the detection and analysis has potential applications in the food industry, particularly detection of food adulteration. The aim of this work was to develop an assay capable of detecting Konjac glucomannan as an isolated sample and within food matrices. An indirect competitive ELISA was developed utilising a polyclonal antibody raised against Konjac glucomannan. The ELISA was found to be specific for Konjac glucomannan and sensitive, with a detection limit of 0.1 ng mL )1 . Increasing salt concentration and freeze ⁄ thaw cycles did not affect the performance of the assay. The ELISA was able to detect Konjac glucomannan in admixtures with other gums and also in confectionery that had been spiked with Konjac glucomannan. The ELISA has potential as a kit for the differentiation of Konjac glucomannan from other hydrocolloids and detection in food.

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Current Awareness in Phytochemical Analysis

2005

Phytochem. Anal.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley &Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of phytochemical analysis. Each bibliography is divided into 13 sections: 1 Books, Reviews &Symposia; 2 General; 3 Nucleic Acids; 4 Amino Acids, Proteins &Enzymes; 5 Carbohydrates; 6 Lipids; 7 Secondary Products; 8 Growth Regulators; 9 Industrially‐Important Products; 10 Toxins/Allergens; 11 Pigments; 12 Vitamins; 13 Others. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted.

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The Development of Immunoassays to Identify and Quantify Species Source of Gum Arabic

Cited by 7 publications

References 13 publications

Generation of genome-scale gene-associated SNPs in catfish for the construction of a high-density SNP array

Generation of genome-scale gene-associated SNPs in catfish for the construction of a high-density SNP array

Detection of Konjac glucomannan by immunoassay

Current Awareness in Phytochemical Analysis

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