The development of Pisum sativum explant systems for studies concerning source‐sink activities

Abstract

Using excised fruit/shoot systems of pea (Pisum sativum L.), the effect of the 10‐day‐old (post anthesis) fruit (sink) on the translocation of 14C‐sucrose applied to the stipule (source) was investigated. We also examined the influence of various growth regulators on the source‐sink relationships using this system. Indole‐3‐yl‐acetic acid (100 or 200 ppm), gibberellic acid (25‐100 ppm) increased 14C‐sucrose translocation into the growing fruit by about 13% and 22–29%, respectively. 2‐chloroethyl phosphonic acid, 6‐benzyladenine and 2‐methyl‐4‐chlorophenoxyacetic acid also increased 14C‐sucrose translocation into the fruit by about 11–26%, 11–23% and 24–37%, respectively. Elevated fruit 14C‐sucrose levels found in other treatments include, methyl‐2‐chloro‐9‐hydroxyfluorene‐9‐carboxylate (300 or 1000 ppm), 2,3,5‐triiodobenzoic acid (100 and 300 ppm) and butanedioic acid mono‐(2,2‐dimethylhydrazide) (30‐300 ppm) by about 34%, 39–52% and 7–13%, respectively.

Abscisic Acid Facilitates Sucrose Import by Strawberry Fruit Explants and Cortex Disks in Vitro

“…Both fruit explants and tissue disks have been used to study assimilate accumulation by sink tissues in vitro (1,4,9,12,14,22). Fruit explants offer the advantage of using an intact, nonwounded tissue.…”