The development of frost tolerance and DHN5 protein accumulation in barley (Hordeum vulgare) doubled haploid lines derived from Atlas 68×Igri cross during cold acclimation

Kosová, Klára; Prášil, Ilja Tom; Prášilová, P.; Vítámvás, Pavel; Chrpová, J.

doi:10.1016/j.jplph.2009.09.020

Cited by 18 publications

(14 citation statements)

References 43 publications

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“…In all samples, the most abundant dehydrin protein was 80 kDa, corresponding to dehydrin 5 (Kosová et al, 2010). The composition of individual dehydrins was different in the two varieties.…”

Section: Resultsmentioning

confidence: 98%

Global Scale Transcriptional Profiling of Two Contrasting Barley Genotypes Exposed to Moderate Drought Conditions: Contribution of Leaves and Crowns to Water Shortage Coping Strategies

et al. 2016

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Drought is a serious threat for sustainable agriculture. Barley represents a species well adapted to environmental stresses including drought. To elucidate the adaptive mechanism of barley on transcriptional level we evaluated transcriptomic changes of two contrasting barley cultivars upon drought using the microarray technique on the level of leaves and crowns. Using bioinformatic tools, differentially expressed genes in treated vs. non-treated plants were identified. Both genotypes revealed tissue dehydration under drought conditions as shown at water saturation deficit and osmotic potential data; however, dehydration was more severe in Amulet than in drought-resistant Tadmor under the same ambient conditions. Performed analysis showed that Amulet enhanced expression of genes related to active plant growth and development, while Tadmor regarding the stimulated genes revealed conservative, water saving strategy. Common reactions of both genotypes and tissues included an induction of genes encoding several stress-responsive signaling proteins, transcription factors as well as effector genes encoding proteins directly involved in stress acclimation. In leaf, tolerant cultivar effectively stimulated mainly the expression of genes encoding proteins and enzymes involved in protein folding, sulfur metabolism, ROS detoxification or lipid biosynthesis and transport. The crown specific reaction of tolerant cultivar was an enhanced expression of genes encoding proteins and enzymes involved in cell wall lignification, ABRE-dependent abscisic acid (ABA) signaling, nucleosome remodeling, along with genes for numerous jasmonate induced proteins.

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Section: Resultsmentioning

confidence: 98%

Global Scale Transcriptional Profiling of Two Contrasting Barley Genotypes Exposed to Moderate Drought Conditions: Contribution of Leaves and Crowns to Water Shortage Coping Strategies

et al. 2016

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“…A large number of genes with various functions are induced during cold acclimation (Laudencia-Chingcuanco et al 2011, Rabbani et al 2003, Seki et al 2002). In particular, cold-responsive ( Cor )/late-embryogenesis-abundant ( Lea ) genes are transcriptionally activated in cold acclimation, and the accumulated COR/LEA proteins lead to protection of the integrity of cell structures and functions from freezing damage (Kosová et al 2010, Thomashow 1999). Most Cor / Lea genes, including Wlt10 , Wdhn13 and Wcor14 , show differential expression levels in two wheat cultivars with contrasting levels of freezing tolerance under low temperature conditions (Kobayashi et al 2004, Laudencia-Chingcuanco et al 2011, Ohno et al 2001, 2003, Tsvetanov et al 2000).…”

Section: Introductionmentioning

confidence: 99%

A major quantitative trait locus for cold-responsive gene expression is linked to frost-resistance gene <i>Fr-A2</i> in common wheat

et al. 2013

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Low temperature induces expression of Cor (cold-responsive)/Lea (late embryogenesis-abundant) gene family members through C-repeat binding factor (CBF) transcription factors in common wheat. However, the relationship between the genetic loci controlling cold-responsive gene expression and freezing tolerance is unclear. In expression quantitative trait locus (eQTL) analysis, accumulated transcripts of Cor/Lea and CBF genes were quantified in recombinant inbred lines derived from a cross between two common wheat cultivars with different levels of freezing tolerance. Four eQTLs controlling five cold-responsive genes were found, and the major eQTL with the greatest effect was located on the long arm of chromosome 5A. At least the 1D and 5A eQTLs played important roles in development of freezing tolerance in common wheat. The chromosomal location of the 5A eQTL, controlling four cold-responsive genes, coincided with a region homoeologous to a frost-tolerance locus (Fr-Am2) reported as a CBF cluster region in einkorn wheat. The 5A eQTL plays a significant role through Cor/Lea gene expression in cold acclimation of wheat. In addition, our results suggest that one or more CBF copies at the Fr-2 region positively regulate other copies, which might amplify the positive effects of the CBF cluster on downstream Cor/Lea gene activation.

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“…Freezing tolerance is associated with the occurrence of a cold acclimation that is triggered by the induction of cold responsive (Cor) genes after exposure of plants to low nonfreezing temperatures for certain periods of time (Winfield et al, 2010). In particular, Cor genes are transcriptionally activated during cold acclimation, and the accumulated COR proteins lead to protection of the integrity and functionality of cell structures from freezing damage (Kosová et al, 2010). Cold acclimation can also lead to changes in lipid composition, protein, and nucleic acid conformation, as well as the accumulation of carbohydrates (Thomashow, 1999;Kosová et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…In particular, Cor genes are transcriptionally activated during cold acclimation, and the accumulated COR proteins lead to protection of the integrity and functionality of cell structures from freezing damage (Kosová et al, 2010). Cold acclimation can also lead to changes in lipid composition, protein, and nucleic acid conformation, as well as the accumulation of carbohydrates (Thomashow, 1999;Kosová et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Such accumulation of sucrose and other simple sugars during cold acclimation contributes to the stabilization of membranes (Thomashow, 1999;Kosová et al, 2010) and may play a key role in protecting the cells from freezing and dehydration (Steponkus, 1984). Sugars are also recognized as important regulatory molecules with both signaling and putative ROS scavenging functions in plants (BolouriMoghaddam et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Nonsense-mediated decay of sucrose synthase 1 mRNA with induced premature chain termination codon during cold acclimation in winter wheat

Armoniené¹,

Brazauskas²

2014

Turk J Bot

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Cold acclimation induces the expression of cold-regulated genes needed to protect plants against freezing damage. Fourteen candidate genes were identified as being differentially expressed (presence/absence) between cold acclimated and nonacclimated crown and leaf tissues of 2 winter wheat lines using the cDNA-AFLP method. Two TILLING populations originating from the 2 winter wheat lines were further developed in order to create mutant forms of the candidate genes to verify their role in freezing-tolerance formation. Exon 8 of the identified differentially expressed sucrose synthase 1 (Ss1) gene was chosen for mutation detection by high-resolution melting analysis. A total of 75.68 kb of DNA was screened and 2 novel alleles of the Ss1 gene were identified, of which 1 was silent and 1 was a nonsense (premature stop codon) mutation. qRT-PCR analysis showed that premature stop codon mutation has a strong negative effect on Ss1 gene expression in nonacclimated leaves as well as in crowns and leaves collected at 2, 4, and 6 weeks of cold acclimation compared with the wild-type winter wheat line. Further work will reveal the effect of the mutation on cold tolerance of winter wheat and will enable the assessment of the role of Ss1 in the cold acclimation process.

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The development of frost tolerance and DHN5 protein accumulation in barley (Hordeum vulgare) doubled haploid lines derived from Atlas 68×Igri cross during cold acclimation

Cited by 18 publications

References 43 publications

Global Scale Transcriptional Profiling of Two Contrasting Barley Genotypes Exposed to Moderate Drought Conditions: Contribution of Leaves and Crowns to Water Shortage Coping Strategies

Global Scale Transcriptional Profiling of Two Contrasting Barley Genotypes Exposed to Moderate Drought Conditions: Contribution of Leaves and Crowns to Water Shortage Coping Strategies

A major quantitative trait locus for cold-responsive gene expression is linked to frost-resistance gene <i>Fr-A2</i> in common wheat

Nonsense-mediated decay of sucrose synthase 1 mRNA with induced premature chain termination codon during cold acclimation in winter wheat

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