The development of fluorescence turn-on probe for Al(III) sensing and live cell nucleus-nucleoli staining

Saini, Anoop Kumar; Sharma, Vinay; Mathur, P. C.; Shaikh, Mobin M.

doi:10.1038/srep34807

Cited by 36 publications

(14 citation statements)

References 43 publications

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“…The nucleolus shows a grainy texture in Fig. 7(c), which conforms with previous works visualizing MCF-7 nucleoli via fluorescence [30]. The surrounding cytoplasm also exhibits significantly increased contrast, and various cytoplasmic extensions and cavities are clearly visible in sharp focus in Fig.…”

Section: D Ri Reconstruction Of Human Breast Adenocarcinomasupporting

confidence: 88%

Refractive index tomography with structured illumination

Chowdhury¹,

Eldridge²,

Wax³

et al. 2017

Optica

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This work introduces a novel reinterpretation of structured illumination (SI) microscopy for coherent imaging that allows three-dimensional imaging of complex refractive index (RI). To do so, we show that coherent SI is mathematically equivalent to a superposition of angled illuminations. It follows that raw acquisitions for standard SI-enhanced quantitative-phase images can be processed into complex electric-field maps describing sample diffraction under angled illuminations. Standard diffraction tomography (DT) computation can then be used to reconstruct the sample's 3D RI distribution at sub-diffraction resolutions. We demonstrate this concept by using a SI-quantitative-phase imaging system to computationally reconstruct 3D RI distributions of human breast (MCF-7) and colorectal (HT-29) adenocarcinoma cells. Our experimental setup uses a spatial light modulator to generate structured patterns at the sample and collects angle-dependent sample diffraction using a common-path, off-axis interference configuration with no moving components. Furthermore, this technique holds promise for easy pairing with SI fluorescence microscopy, and important future extensions may include multimodal, sub-diffraction resolution, 3D RI and fluorescent visualizations.

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Section: D Ri Reconstruction Of Human Breast Adenocarcinomasupporting

confidence: 88%

Refractive index tomography with structured illumination

Chowdhury¹,

Eldridge²,

Wax³

et al. 2017

Optica

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“…The binding mode of TPySSB with Al 3+ was also studied by analyzing electrospray ionization MS spectra. A characteristic peak at m/z 580.0779, corresponding to [TPySSB + 2Al 3+ + NO 3 − + 2H 2 O -H + ] + , 23 is present in the positive-ion mass spectrum of TPySSB-Al 3+ complex, confirming that the stoichiometry ratio between TPySSB and Al 3+ is 1:2. Figure 9 shows the 1 H NMR spectra of single TPySSB and complex TPySSB-Al 3+ .…”

Section: Proposed Binding Mechanismmentioning

confidence: 72%

A Highly Selective Fluorescent Chemosensor for Al3+ Based on 2,2’:6’,2”-Terpyridine with a Salicylal Schiff Base

Xu¹,

Li²,

Li³

et al. 2020

J. Braz. Chem. Soc.

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Two 2,2':6',2"-terpyridine-based Schiff bases (TPySSB and TPySB) have been synthesized. The TPySSB shows remarkable selective 'off-on' fluorescence for Al 3+ in combination with photoinduced electron transfer (PET) mechanism and C=N isomerization mechanism. Chemosensor TPySSB binds Al 3+ in a 1:2 ratio with an association constant 6.8 × 10 5 (correlation coefficient (R 2) = 0.98) and this 1:2 stoichiometric model is established on Job's plot and 1 H nuclear magnetic resonance (NMR). Comparing TPySSB and TPySB, it is of great importance the existence of oxygen atom of hydroxyl group in salicylidene due to its strong binding abilities of both phenol and C=N structure to the Al 3+ .

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“…The nucleolus is the site for rRNA transcription and for ribosomal subunit assembly . To determine the interaction between HL1 and RNA, we performed RNase digestion tests in HeLa cells, after incubating with HL1 and zinc salts , , . the RNase enzyme hydrolyzes RNA, which is present in the cells.…”

Section: Resultsmentioning

confidence: 99%

Purine‐Based Fluorescent Sensors for Imaging Zinc Ions in HeLa Cells

et al. 2017

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We synthesized three chelate‐tethered purine ligands, having a Schiff base linkage at the N9 position, which resulted in the dipolar imine‐methoxyphenol adenine Schiff base HL1, the 2‐aminopurine Schiff base HL2, and the 2,6‐diaminopurine Schiff base HL3. These ligands selectively showed enhancement of the fluorescence of the zinc ions. The imine‐based compound HL1 chelated with zinc ions to give the discrete dimeric structure 1, a green fluorescent complex, which was again stabilized by purine–purine interactions to give a 1D polymeric structure. The ligands having a 6‐amino group, HL1 and HL3, have high quantum yield, which gives higher fluorescence enhancement of the free zinc ions in the HeLa cells. HL1 was localized in the cytoplasm and the nucleoli of the HeLa cells, which was confirmed by an RNase digestion test.

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The development of fluorescence turn-on probe for Al(III) sensing and live cell nucleus-nucleoli staining

Cited by 36 publications

References 43 publications

Refractive index tomography with structured illumination

Refractive index tomography with structured illumination

A Highly Selective Fluorescent Chemosensor for Al3+ Based on 2,2’:6’,2”-Terpyridine with a Salicylal Schiff Base

Purine‐Based Fluorescent Sensors for Imaging Zinc Ions in HeLa Cells

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