Purpose The aim of the study was to identify neurodevelopmental disorders and difficulties (NDD) in a male prison. The study used standardised tools to carry out screening and diagnostic assessment of the attention deficit hyperactivity disorder (ADHD), autism spectrum disorder (ASD) and intellectual disability (ID).Method The adult ADHD self-report scale (ASRS), 20-item Autism Quotient (AQ-20) and the Learning Disability Screening Questionnaire (LDSQ) were used to screen 240 male prisoners.Prisoners who screened positive on one or more of these scales or self-reported a diagnosis of ADHD, ASD or ID were further assessed using the Diagnostic Interview for ADHD in Adults (DIVA), adapted Autism Diagnostic Observation Schedule (ADOS) and the Quick Test.Findings Of the 87 prisoners who screened positive for NDD and were further assessed, 70 met the study's diagnostic criteria for ADHD, ASD or ID. Most of those with NDD (51%) had previously gone unrecognised and a high proportion (51%) were identified through staff-or self-referral to the study.Value The study demonstrated that improving awareness and providing access to skilled, standardised assessment within a male prison can result in increased recognition and identification of NDD.