The development of embryo sacs in in vitro ovaries of Nicotiana tabacum L

Lobanova, L. P.; Enaleeva, N. Kh.

doi:10.1016/s0168-9452(98)00009-0

Cited by 5 publications

(3 citation statements)

References 6 publications

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“…Hand pollination using mature pollen can be performed on immature flowers ( Haring et al, 1990 ) in an attempt to bypass the effects of self-incompatibility on species of Brassicaceae ( Hiscock and Dickinson, 1993 ), as well as Solanaceae species ( Chalivendra et al, 2013 ). In Nicotiana tabacum , a species with a Polygonum-type embryo sac ( Huang and Russell, 1992 ), the ovule is not fully developed at anthesis, and the egg cell is not usually observed at the embryo sacs ( Tian and Russell, 1997 ; Lobanova and Enaleeva, 1998 ; De Martinis and Mariani, 1999 ; Chen et al, 2012 ). In this species, the effects of pollination on ovule development have mainly been examined at stages close to anthesis.…”

Section: Introductionmentioning

confidence: 99%

Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers

et al. 2015

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In Nicotiana tabacum, female gametophytes are not fully developed at anthesis, but flower buds pollinated 12 h before anthesis produce mature embryo sacs. We investigated several pollination-associated parameters in N. tabacum flower buds to determine the developmental timing of important events in preparation for successful fertilization. First, we performed hand pollinations in flowers from stages 4 to 11 to study at which developmental stage pollination would produce fruits. A Peroxtesmo test was performed to correlate peroxidase activity on the stigma surface, indicative of stigma receptivity, with fruit set. Pollen tube growth and female gametophyte development were microscopically analyzed in pistils of different developmental stages. Fruits were obtained only after pollinations of flower buds at late stage 7 and older; fruit weight and seed germination capacity increased as the developmental stage of the pollinated flower approached anthesis. Despite positive peroxidase activity and pollen tube growth, pistils at stages 5 and 6 were unable to produce fruits. At late stage 7, female gametophytes were undergoing first mitotic division. After 24 h, female gametophytes of unpollinated pistils were still in the end of the first division, whereas those of pollinated pistils showed egg cells. RT-qPCR assay showed that the expression of the NtEC1 gene, a marker of egg cell development, is considerably higher in pollinated late stage 7 ovaries compared with unpollinated ovaries. To test whether ethylene is the signal eliciting female gametophyte maturation, the expression of ACC synthase was examined in unpollinated and pollinated stage 6 and late stage 7 stigmas/styles. Pollination induced NtACS expression in stage 6 pistils, which are unable to produce fruits. Our results show that pollination is a stimulus capable of triggering female gametophyte development in immature tobacco flowers and suggests the existence of a yet undefined signal sensed by the pistil.

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Section: Introductionmentioning

confidence: 99%

Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers

et al. 2015

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“…Нами (на кафедре генетики Саратовского государственного университета) был предложен экспериментальный подход, открывающий возможность дискретного исследования реакции каждой стадии макрогаметофитогенеза на экспериментальные воздействия. Он состоит в изоляции завязей на любой стадии развития и культивировании их в условиях in vitro [9]. Это позволило создать строго контролируемые условия, в том числе температурные, и подвергать одновременному воздействию большое количество завязей на одинаковой стадии развития.…”

Section: материалы и методыunclassified

The Specifics of Tobacco Gametophyte Mutations Display Depending on Temperature

Nurusheva¹,

Lobanova²,

Kolesova³

et al. 2017

CBE

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“…Then, three megaspores degenerate and one megaspore develops into an embryo sac (Lobanova and Enaleeva 1998). In the present study, monosomics were obtained at the same rate as disomics from the cross Haplo-Q × Samsun NN.…”

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confidence: 95%

Development of Q-chromosome-specific DNA Markers in Tobacco and Their Use for Identification of a Tobacco Monosomic Line

Tezuka

Onosato

Hijishita

et al. 2004

Plant and Cell Physiology

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;We developed seven Q-chromosome-specific DNA markers in Nicotiana tabacum by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis using two hybrid lines, and we were able to identify tobacco monosomic plants among F 1 progeny derived from the cross N. tabacum Haplo-Q × N. tabacum cv. Samsun NN using Q-chromosome-specific DNA markers. Based on the results, we discuss the roles of the Q chromosome in embryo sac development and embryogenesis. Here, we propose a new method for identifying DNA markers for a particular chromosome in the genus Nicotiana.

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The development of embryo sacs in in vitro ovaries of Nicotiana tabacum L

Cited by 5 publications

References 6 publications

Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers

Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers

The Specifics of Tobacco Gametophyte Mutations Display Depending on Temperature

Development of Q-chromosome-specific DNA Markers in Tobacco and Their Use for Identification of a Tobacco Monosomic Line

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