The Development of DNA Sequencing: From the Genome of a Bacteriophage to That of a Neanderthal

Abstract

Them bones gonna rise again: Only a few weeks ago the Neandertal genome, deciphered from small amounts of ancient DNA, was published. This mileston was possible thanks to tremendous improvements in DNA‐sequencing technologies. The picture shows a bone fragment from which genomic DNA was isolated for the sequencing experiment. (Picture copyright: Max‐Planck‐Institute of Evolutionary Anthropology).

Phytoplankton community structure and diversity in the indoor industrial aquaculture system for Litopenaeus vannamei revealed by high‐throughput sequencing and morphological identification

“…Therefore, microscopy is potentially vulnerable to subjective judgments and requires advanced taxonomic skills and expertise. With the development of DNA sequencing technology (Sundermann, Kushnir, & Schulz, 2010), the high-throughput sequencing approach has already been successfully applied for the assessment of phytoplankton community structure and diversity (Mäki, Salmi, Mikkonen, Kremp, & Tiirola, 2017;Oliveira et al, 2018;Penna, Casabianca, Guerra, Vernesi, & Scardi, 2017). High-throughput sequencing surveys have advantages over traditional morphological methods due to their cost and time savings, higher resolution and lower detection threshold (Reuter, Spacek, & Snyder, 2015).…”

Hydrodynamic shearing of DNA in a polymeric microfluidic device

“…5 A key sample preparation step required for any NGS system is the random fragmentation of chromosomal DNA into appropriate fragment sizes possessing blunt ends. 6,7 Several protocols for DNA fragmentation have been established and realized in bench-top commercial systems. Unlike bench-top ultrasonic-based fragmentation approaches (Covaris Ò ) for which integration into a microfluidic is technically problematic and an additional step of end-repair is required post-fragmentation, 8 hydrodymnanic shearing appears to be more suitable for this purpose because it generates random blunt-end fragments with a relatively narrow size distribution that are suitable for sequencing library construction.…”