The development of a semi-preparatory scale supercritical-fluid chromatograph for high-throughput purification of ‘combi-chem’ libraries

Berger, Terry A.; Fogleman, Kimber; Staats, Thomas; Bente, P. F.; Crocket, Ivan; Farrell, William; Osonubi, Michael

doi:10.1016/s0165-022x(00)00084-1

Cited by 54 publications

(31 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We tried small acids, such as 0.1% (v/v) trifluoroacetic acid in methanol, and small bases, such as 0.1% isopropylamine and 0.4% dimethylethylamine in methanol. Our results were consistent with previous work with these small acids and bases as mobile-phase additives [20]. Small bases improved the peak shapes of bases, while small acids provided improvements for acidic analytes.…”

Section: Resultssupporting

confidence: 92%

Elution and preliminary structure‐retention modeling of polar and ionic substances in supercritical fluid chromatography using volatile ammonium salts as mobile phase additives

Pinkston¹,

Stanton

Wen

2004

J of Separation Science

View full text Add to dashboard Cite

Supercritical fluid chromatography has primarily been applied to relatively nonpolar analytes, even when polar organic solvents are used as modifiers. Here, we show that low levels of volatile ammonium salts as mobile-phase additives allow the elution of polar and even ionic organic materials such as sulfonate salts, carboxylate salts, polyamines, and quaternary ammonium salts. Also, volatile ammonium salts are compatible with mass spectrometric detection, in contrast to other common additives. We have performed preliminary structure-activity-relationship modeling for retention in the CO2/methanol/NH4OAc/Deltabond Cyano system. We have developed a three-descriptor model, where one descriptor, the "relative negative charged surface" explains over 61% of the variance in the retention values. We suggest that two mechanisms have the greatest influence on retention in this system. One is related to the presence of a volatile ammonium salt, the other is related to the ability of a molecule to "hide" its atom with the greatest partial negative charge.

show abstract

Section: Resultssupporting

confidence: 92%

Elution and preliminary structure‐retention modeling of polar and ionic substances in supercritical fluid chromatography using volatile ammonium salts as mobile phase additives

Pinkston¹,

Stanton

Wen

2004

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Rapid identification of specific enzymes for transformations and optimal reaction conditions leads to accelerated drug development. Due to the advancement in instrumentation, SFC has been widely used in the pharmaceutical industry for analytical, preparative, and chiral separations [29]. One of the advantages of chiral SFC as compared to chiral HPLC or chiral GC is its separation efficiency [30].…”

Section: Resultsmentioning

confidence: 99%

Rapid, automated screening method for enzymatic transformations using a robotic system and supercritical fluid chromatography

McConnell²,

Kerns

et al. 2004

Journal of Chromatography B

View full text Add to dashboard Cite

“…161 The SFC semipreparative chromatograph for the high-throughput purification of combi-chem libraries was described in detail by Berger in 2000. 162 An example of a commercially available automated system is the SF3Ô supercritical fluid chromatography system manufactured by Gilson. This system is suitable for processing up to 108 samples for analytical runs and up to 60 samples for semipreparative runs.…”

Section: Characterization and Purificationmentioning

confidence: 99%

Practical Aspects of Combinatorial Solid‐Phase Synthesis

Hlaváč¹,

Soural²,

Krchňák³

2011

Solid‐Phase Organic Synthesis

View full text Add to dashboard Cite

The development of a semi-preparatory scale supercritical-fluid chromatograph for high-throughput purification of ‘combi-chem’ libraries

Cited by 54 publications

References 17 publications

Elution and preliminary structure‐retention modeling of polar and ionic substances in supercritical fluid chromatography using volatile ammonium salts as mobile phase additives

Elution and preliminary structure‐retention modeling of polar and ionic substances in supercritical fluid chromatography using volatile ammonium salts as mobile phase additives

Rapid, automated screening method for enzymatic transformations using a robotic system and supercritical fluid chromatography

Practical Aspects of Combinatorial Solid‐Phase Synthesis

Contact Info

Product

Resources

About