The development of a new three-step protocol to determine the efficacy of disinfectant wipes on surfaces contaminated with Staphylococcus aureus

Williams, Gareth; Denyer, Stephen Paul; Hosein, I. K.; Hill, D. W.; Maillard, Jean‐Yves

doi:10.1016/j.jhin.2007.08.012

Cited by 51 publications

(81 citation statements)

References 8 publications

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“…S. aureus, E. coli, and PV-1 counts on fomites were reduced by 3.5 to 5.0 log 10 , whereas B. thuringiensis spore counts were reduced by up to only 2.5 log 10 . These findings are consistent with previous studies that showed that disinfectant wipes reduce the microbial load on contaminated surfaces (22,29,30,(49)(50)(51). The present study demonstrated B. thuringiensis spores to be more resistant to physical removal and/or inactivation by disinfectant wipes than nonspore-forming bacteria, which is consistent with the findings of Siani et al (22) for Clostridium difficile spores.…”

Section: Discussionsupporting

confidence: 93%

“…However, an earlier study suggests that the presence of a higher organic material on surfaces can reduce the ability of the wipes to remove bacteria (29). Several studies have tested the efficacy of disinfectant wipes by the method described by Williams et al (29) and found results similar to those of the present study, showing microbial reduction on fomites after wiping with a disinfectant wipe (22,29,30,49).…”

Section: Discussionsupporting

confidence: 80%

“…The six swatches that were to be treated with the disinfectant wipe were taken one at a time from the incubator, placed on the counter, and held stationary with the left hand. Subsequently, the swatch was treated with a disinfectant wipe applied in one application and one sweeping motion in one direction (from the far corner of the swatch toward the proximal corner) in a pressure range of 300 to 500 g/cm 2 (22,29,30,49). The application of the disinfectant wipe was approximately 1 s long, as previously described (50).…”

Section: Seeding Of Fomites (I) Layout Of Fomitesmentioning

confidence: 99%

“…The potential for pathogen transfer from contaminated fomites to fingers is a concern in health care environments; particularly those in close proximity to the patient that are frequently touched (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25). Health care-acquired infections caused by methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus, and Clostridium difficile are associated with high morbidity and mortality (18,20,22,(26)(27)(28)(29)(30). Most nosocomial and foodborne pathogens can persist on fomites for weeks or even months (18,19,31,32) and on fingers for up to several hours (33)(34)(35).…”

mentioning

confidence: 99%

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Evaluation of a Disinfectant Wipe Intervention on Fomite-to-Finger Microbial Transfer

Lopez

Kitajima

Havas

et al. 2014

Appl Environ Microbiol

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bInanimate surfaces, or fomites, can serve as routes of transmission of enteric and respiratory pathogens. No previous studies have evaluated the impact of surface disinfection on the level of pathogen transfer from fomites to fingers. Thus, the present study investigated the change in microbial transfer from contaminated fomites to fingers following disinfecting wipe use. Escherichia coli (10 8 to 10 9 CFU/ml), Staphylococcus aureus (10 9 CFU/ml), Bacillus thuringiensis spores (10 7 to 10 8 CFU/ml), and poliovirus 1 (10 8 PFU/ml) were seeded on ceramic tile, laminate, and granite in 10-l drops and allowed to dry for 30 min at a relative humidity of 15 to 32%. The seeded fomites were treated with a disinfectant wipe and allowed to dry for an additional 10 min. Fomite-to-finger transfer trials were conducted to measure concentrations of transferred microorganisms on the fingers after the disinfectant wipe intervention. The mean log 10 reduction of the test microorganisms on fomites by the disinfectant wipe treatment varied from 1.9 to 5.0, depending on the microorganism and the fomite. Microbial transfer from disinfectant-wipetreated fomites was lower (up to <0.1% on average) than from nontreated surfaces (up to 36.3% on average, reported in our previous study) for all types of microorganisms and fomites. This is the first study quantifying microbial transfer from contaminated fomites to fingers after the use of disinfectant wipe intervention. The data generated in the present study can be used in quantitative microbial risk assessment models to predict the effect of disinfectant wipes in reducing microbial exposure. Inanimate objects and surfaces (fomites) are known to be a reservoir for the transmission of pathogens in the environment directly, by surface contact with the mouth or abraded skin, or indirectly by contamination of fingers and subsequent hand-to-mouth, hand-toeye, or hand-to-nose contact (1, 2). Previous laboratory studies have modeled food preparation in domestic kitchens to better understand cross-contamination of food-borne pathogens (3, 4). The occurrence and spread of pathogens throughout the home and health care settings have also been studied to better understand the role of fomites in pathogen exposure and acquired infections (5-11). The potential for pathogen transfer from contaminated fomites to fingers is a concern in health care environments; particularly those in close proximity to the patient that are frequently touched (12-25). Health care-acquired infections caused by methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus, and Clostridium difficile are associated with high morbidity and mortality (18,20,22,(26)(27)(28)(29)(30). Most nosocomial and foodborne pathogens can persist on fomites for weeks or even months (18,19,31,32) and on fingers for up to several hours (33-35). Pathogen presence and survival on fomites in domestic homes, public places, hospitals, and other health care facilities are important factors in evaluating potential health risks t...

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 80%

Section: Seeding Of Fomites (I) Layout Of Fomitesmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of a Disinfectant Wipe Intervention on Fomite-to-Finger Microbial Transfer

Lopez

Kitajima

Havas

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Removal of bacteria from a model healthcare solid surface was tested based on methodology reported by Williams et al (5). For brevity, only modifications to this protocol are described.…”

Section: 34mentioning

confidence: 99%