“…Different determination methods have been reported for the analysis of histamine, including thin-layer chromatography (LapaGuimarães & Pickova, 2004;Lieber & Taylor, 1978;Shakila, Vasundhara, & Kumudavally, 2001), gas chromatography (Hwang, Wang, & Choong, 2003), colorimetric assay (Hardy & Smith, 1976;Kawabata, Uchida, & Akano, 1960), fluorometric assay (AOAC, 2002), enzymaticassay (Lopez-Sabater, Rodriguez-Jerez, Roig-Sagues, & Mora-Ventura, 1994), immunological assay (Serrar, Brebant, Bruneau, & Denoyel, 1995) and high-performance liquid chromatography (Jensen & Marley, 1995;Jeyashakila, Vasundhara, & Kumudavally, 2001;Sato et al, 1995;Yen & Hsieh, 1991). Concerning the determination of histamine, it is a very serious problem to separate histamine completely from a very large amount of interference compounds such as histidine or carnosine.…”