The development of a molecular approach for coprodiagnosis of Toxoplasma gondii

Salant, Harold; Markovics, Alex; Spira, Dan T.; Hamburger, Joseph

doi:10.1016/j.vetpar.2007.02.022

Cited by 37 publications

(32 citation statements)

References 28 publications

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“…The copro-PCR, by the nature of the technology, is highly reproducible and objective, as suggested by results obtained when multiple samples were examined in this study and in other spiking experiments. 27 By comparison, the bioassay for testing mice, although potentially able to detect one oocyst per infective dose 24, 37 is cumbersome, expensive, and usually requires six weeks before tissue cysts can be observed. 27 Therefore, it cannot be considered suitable for large-scale screening.…”

Section: Discussionmentioning

confidence: 99%

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A Comparative Analysis of Coprologic Diagnostic Methods for Detection of Toxoplama gondii in Cats

Salant¹,

Spira²,

Hamburger³

2010

The American Journal of Tropical Medicine and Hygiene

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Abstract. The relative role of transmission of Toxoplasma gondii infection from cats to humans appears to have recently increased in certain areas. Large-scale screening of oocyst shedding in cats cannot rely on microscopy because oocyst identification lacks sensitivity and specificity, or on bioassays, which require test animals and weeks before examination. We compared a sensitive and species-specific coprologic-polymerase chain reaction (copro-PCR) for detection of T. gondii infected cats with microscopy and a bioassay. In experimentally infected cats followed over time, microscopy was positive occasionally, and positive copro-PCR and bioassay results were obtained continuously from days 2 to 24 postinfection. The copro-PCR is at least as sensitive and specific as the bioassay and is capable of detecting infective oocysts during cat infection. Therefore, this procedure can be used as the new gold standard for determining potential cat infectivity. Its technologic advantages over the bioassay make it superior for large-scale screening of cats.

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Section: Discussionmentioning

confidence: 99%

“…27 This coprologic-PCR (copro-PCR) uses primers that amplify a 529-basepair sequence that is present 200-300 times in the T. gondii genome. Although this report described a valid test for T. gondii detection, a comparative analysis was still required.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Analysis of Coprologic Diagnostic Methods for Detection of Toxoplama gondii in Cats

Salant¹,

Spira²,

Hamburger³

2010

The American Journal of Tropical Medicine and Hygiene

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“…These findings were reported elsewhere, despite some descriptions of persistent excretion in some models, ascribed to strains or parasite variation (DUBEY, 2005). Specific IgG serology only becomes evident after oocyst excretion, which results in poor diagnostic value for individual felids that are implicated as a source of T. gondii spreading, but it is a feasible technique for feline toxoplasmosis prevalence, with results related to a past excretion of oocysts and chronic infection (MEIRELES et al, 2004, SALANT et al, 2007.…”

Section: Discussionmentioning

confidence: 80%

“…Oocyst preservation in KKK also allows gross genera identification, due to the preservation of oocyst diameter, but more accurate studies other than morphology must be performed for adequate speciation, as elsewhere described (SHARES et al, 2005). Without staining, the dried slide could be an alternative for nucleic acid preservation for genomic speciation, which could be performed by nucleic acid purification and adequate primers in PCR (SALANT et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Quantitative toxoplasma gondii oocyst detection by a modified Kato Katz test using Kinyoun staining (KKK) in ME49 strain experimentally infected cats

Meireles

Tsutsui

Carmo

et al. 2008

Rev. Inst. Med. trop. S. Paulo

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SUMMARYWe detected Toxoplasma gondii oocysts in feces of experimentally infected cats, using a Kato Katz approach with subsequent Kinyoun staining. Animals serologically negative to T. gondii were infected orally with 5x10 2 mice brain cysts of ME49 strain. Feces were collected daily from the 3 rd to the 30 th day after challenge. Oocysts were detected by qualitative sugar flotation and the quantitative modified Kato Katz stained by Kinyoun (KKK). In the experimentally infected cats, oocysts were detected from the 7 th to 15 th day through sugar flotation technique, but oocysts were found in KKK from the 6 th to 16 th day, being sensitive for a larger period, with permanent documentation. The peak of oocysts excretion occurred between the 8 th to 11 th days after challenge, before any serological positive result. KKK could be used in the screening and quantification of oocysts excretion in feces of suspected animals, with reduced handling of infective material, decreasing the possibility of environmental and operator contamination.

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“…A técnica baseia-se em flotação de material fecal em solução de Sheather, seguida de ruptura mecânica em nitrogênio líquido, posterior extração de DNA do extrato proveniente das fezes e por fim análise do material pela PCR convencional, o que proporciona a detecção de um a dois oocistos de T. gondii provenientes das fezes de gatos (SALANT et al, 2007).…”

Section: Diagnóstico Para a Detecção De Oocistosunclassified

INFECÇÃO PELO Toxoplasma gondii E PELO VÍRUS DA IMUNODEFICIÊNCIA FELINA EM GATOS DOMÉSTICOS (Felis catus)

Costa¹,

Jayme²,

Linhares³

et al. 2015

Enciclopédia Biosfera

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RESUMOO gato doméstico (Felis catus) é um animal de companhia, com crescente valorização como pet, o que tem proporcionado o aumento de sua população nos domicílios brasileiros. Apresenta elevada importância na Saúde Pública, participando do ciclo de algumas zoonoses, dentre as quais a toxoplasmose. Constitui-se em um dos hospedeiros definitivos do Toxoplasma gondii, protozoário responsável por esta enfermidade. O gato doméstico também pode ser acometido pelo vírus da imunodeficiência felina (FIV) e devido à imunossupressão causada por tal patógeno pode vir a desenvolver doenças oportunistas. A infecção simultânea do T. gondii e o FIV pode predispor ao desenvolvimento agudo da toxoplasmose nos felídeos. Nesta revisão foram abordados aspectos etiológicos, epidemiológicos, clínicos, de diagnóstico e profiláticos destas duas enfermidades. Maior enfoque foi dado aos resultados encontrados no Brasil e no mundo relativos à frequência de anticorpos anti-T. gondii, à detecção de oocistos do T. gondii nas fezes destes animais, a dados sorológicos para o vírus da imunodeficiência felina em gatos domésticos, à coinfecção entre estes dois patógenos, além dos possíveis fatores associados às infecções. Tal abordagem buscou demonstrar o impacto das duas infecções na Medicina Veterinária e na Saúde Pública. PALAVRAS-CHAVE: AIDS felina, frequência, oocisto, toxoplasmose. Toxoplasma gondii INFECTION AND THE FELINE IMMUNODEFICIENCY VIRUS IN DOMESTIC CATS (Felis catus) -LITERATURE REVIEWABSTRACT The domestic cat (Felis catus) is a companion animal, with growing appreciation as a pet, which has provided the increase of its population in Brazilian households. It has high importance in Public Health, participating in the cycle of some zoonoses, among which toxoplasmosis. It is one of the definitive hosts of Toxoplasma gondii parasite responsible for this disease. The domestic cat can also be affected by feline immunodeficiency virus (FIV) and due to immunosuppression caused by this pathogen can go on to develop opportunistic infections. Simultaneous infection of T.

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The development of a molecular approach for coprodiagnosis of Toxoplasma gondii

Cited by 37 publications

References 28 publications

A Comparative Analysis of Coprologic Diagnostic Methods for Detection of Toxoplama gondii in Cats

A Comparative Analysis of Coprologic Diagnostic Methods for Detection of Toxoplama gondii in Cats

Quantitative toxoplasma gondii oocyst detection by a modified Kato Katz test using Kinyoun staining (KKK) in ME49 strain experimentally infected cats

INFECÇÃO PELO Toxoplasma gondii E PELO VÍRUS DA IMUNODEFICIÊNCIA FELINA EM GATOS DOMÉSTICOS (Felis catus)

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