The development of a microassay for human committed progenitor cells

Abstract

A microassay for human committed progenitor cells (CFU-c) has been developed using 24-well, 16 mm diameter culture dishes. Comparisons were made of simultaneous cultures of 21 samples in both 35 mm and 16 mm culture dishes employing two sources of colony-stimulating factor (CSF). The microassay does not differ significantly from the standard 1 ml 35 mm assay, apart from some enhancement of colony numbers in the 16 mm dish. Other advantages of the microassay are that it is economical with respect to cells, media, and space; and it is possible to increase the number of experiments fivefold which can be performed with the same number of cells.

A human complement‐fixing monoclonal anti‐human lymphocyte antibody of rat origin

“…Percoll-isolated bone marrow cells or Ficollisolated PBMC from patients with beta-thalassaemia were used as a readily available source of colony-forming cells and were plated in 16 mm wells of 24-well tissue culture plates (Costar, Cambridge, Mass) as described previously (20). Brielly, 2-0 x lO"^ cells in 200 /il of a-MEM and 0-35% agar were plated into each of five wells containing irradiated leucocyte feeder underlayers in a-MEM and 0-5% agar.…”