The development of a hydrodynamic flow assisted double junction interface for signal improvement in capillary electrophoresis–mass spectrometry using positively charged nonvolatile additives

Wang, Nan Hsuan; Her, Guor‐Rong

doi:10.1016/j.chroma.2014.12.046

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…highly sulfated γ‐cyclodextrin , or micelles were presented. Double junction interface introduced by Wang and Her represents more challenging approach for MEKC‐MS analyses requiring thorough optimization of electroosmotic and hydrodynamic flow, with respect to analytes and micelles mobilities, to retain the micelles in the separation capillary .…”

Section: Development Of Methodology and Instrumentationmentioning

confidence: 99%

“…Moreover, this multichannel design might be a promising approach for online postcolumn derivatization in the future. Generally, the construction of liquid junction interfacing leads to a significant decrease of the analyte dilution . Unfortunately, reproducible gap adjustment between the emitter and the separation capillary is a limitation in the routine work.…”

Section: Development Of Methodology and Instrumentationmentioning

confidence: 99%

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Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

2016

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This review focuses on the latest development of microseparation electromigration methods in capillaries and microfluidic devices coupled with MS for detection and identification of important analytes. It is a continuation of the review article on the same topic by Kleparnik (Electrophoresis 2015, 36, 159-178). A wide selection of 161 relevant articles covers the literature published from June 2014 till May 2016. New improvements in the instrumentation and methodology of MS interfaced with capillary or microfluidic versions of zone electrophoresis, isotachophoresis, and isoelectric focusing are described in detail. The most frequently implemented MS ionization methods include electrospray ionization, matrix-assisted desorption/ionization and inductively coupled plasma ionization. Although the main attention is paid to the development of instrumentation and methodology, representative examples illustrate also applications in the proteomics, glycomics, metabolomics, biomarker research, forensics, pharmacology, food analysis, and single-cell analysis. The combinations of MS with capillary versions of electrochromatography, and micellar electrokinetic chromatography are not included.

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Section: Development Of Methodology and Instrumentationmentioning

confidence: 99%

Section: Development Of Methodology and Instrumentationmentioning

confidence: 99%

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

2016

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“…Recently, to resolve the problems resulting from using nonvolatile additives, Wang et al. proposed a hydrodynamic flow assisted double junction capillary CE‐MS interface, in which the ion suppression due to nonvolatile negative ion additives modified could be alleviated. Using this setup, CE‐MS analysis of positively charged triazines was performed by using cetyltrimethylammonium ion (CTA + ) as an additive.…”

Section: Detectionmentioning

confidence: 99%

Recent advances in microscale separation

et al. 2017

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Recent advances in microscale separationThis review focuses on the recent advances on microscale separation technology, particularly on the new development on column and detection technologies in electrophoretic and chromatographic separation modes (i.e., CE, CEC, cLC, etc.). Representative examples illustrate also applications on the small molecules, peptides, proteins, polysaccharides, chiral and so on. 246 relevant articles are cited covering the literature published

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A robust sheath‐flow CE‐MS interface for hyphenation with Orbitrap MS

2020

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The hyphenation of capillary electrophoresis with high‐resolution mass spectrometry, such as Orbitrap MS, is of broad interest for the unambiguous and exceptionally sensitive identification of compounds. However, the coupling of these techniques requires a robust ionization interface that does not influence the stability of the separation voltage while coping with oxidation of the emitter tip at large ionization voltages. Herein, we present the design of a sheath‐flow CE‐ESI‐MS interface which combines a robust and easy to operate set‐up with high‐resolution Orbitrap MS detection. The sheath liquid interface is equipped with a gold coated electrospray emitter which increases the stability and overall lifetime of the system. For the characterization of the interface, the spray stability and durability were investigated in dependence of the sheath‐flow rate, electrospray voltage, and additional gold coating. The optimized conditions were applied to a separation of angiotensin II and neurotensin resulting in LODs of 2.4 and 3.5 ng/mL.

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The development of a hydrodynamic flow assisted double junction interface for signal improvement in capillary electrophoresis–mass spectrometry using positively charged nonvolatile additives

Cited by 4 publications

References 29 publications

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

Recent advances in microscale separation

A robust sheath‐flow CE‐MS interface for hyphenation with Orbitrap MS

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