The Development of a Genetic Profile of Placental Gene Expression during the First Trimester of Pregnancy: A Potential Tool for Identifying Novel Secreted Markers

Page, Nigel M.; Butlin, David; Manyonda, Isaac; Lowry, P. J.

doi:10.1159/000021014

Cited by 15 publications

(5 citation statements)

References 17 publications

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“…Approaches to providing a positive control include use of distinguishable tracer DNA (18 ) or detection of fetally expressed genes to confirm the presence of fetally derived nucleic acid in plasma extracts (36 ). Placenta-specific mRNA expression profiles have recently been reported (37,38 ); alternatively, a combination of methylation patterns from imprinting and known inherited polymorphisms can be exploited to identify fetal material specifically (39 ).…”

Section: Discussionmentioning

confidence: 99%

Accurate and Robust Quantification of Circulating Fetal and Total DNA in Maternal Plasma from 5 to 41 Weeks of Gestation

Birch¹,

English²,

O’Donoghue

et al. 2005

Clinical Chemistry

132

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Background: Detection of fetal DNA in maternal plasma is achievable at 5 weeks of gestation, but few large-scale studies have reported circulating fetal and maternal DNA across all trimesters. Methods: Blood samples were collected from 201 women between 5 and 41 weeks of pregnancy. Quantitative PCR was used to assess total and fetal DNA concentrations, and allelic discrimination analysis was investigated as a route to detecting specifically fetal DNA. Results: Male fetuses were detectable from 5 weeks amenorrhea with increasing fetal DNA concentrations across gestation. The sensitivity of fetal male gender determination in pregnancies with live birth confirmation was 99%, with 100% specificity. Total DNA concentrations did not correlate with gestational age, but appeared slightly higher in the first and third trimesters than in mid-pregnancy. Analysis of short tandem repeats demonstrated that significant improvements in the detection limit are required for specific detection of fetal DNA. Conclusions: The high sensitivity of PCR-based detection, together with quantification provided by real-time DNA analysis, has clear potential for clinical application in noninvasive prenatal diagnosis. However, accurate quantification using best-fit data analysis, standardization of methods, and performance control

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Section: Discussionmentioning

confidence: 99%

Accurate and Robust Quantification of Circulating Fetal and Total DNA in Maternal Plasma from 5 to 41 Weeks of Gestation

Birch¹,

English²,

O’Donoghue

et al. 2005

Clinical Chemistry

132

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show abstract

“…All total RNA samples were treated with DNase I to remove any contaminating DNA and re-purified as above. The final amount and purity of total RNA was determined as previously described (19). A total of 1.5 g of each purified total RNA was subjected to first strand cDNA synthesis using the SMART RACE amplification kit (CLONTECH Laboratories, Inc. Basingstoke, UK) to produce 3Ј SMART RACE cDNA as previously described (20).…”

Section: Identification Of Tachykinin Receptors In the Placenta Mediamentioning

confidence: 99%

Neurokinin B Is a Paracrine Vasodilator in the Human Fetal Placental Circulation

Brownbill

Bell

Woods

et al. 2003

The Journal of Clinical Endocrinology & Metabolism

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Neurokinin (NK) B is a member of the tachykinin family of neurotransmitters, exerting hypotensive or hypertensive effects in the mammalian vasculature through synaptic release from peripheral neurons, according to either NK(1) and NK(2) or NK(3) receptor subtype expression, respectively. There is recent evidence that NKB is expressed by the syncytiotrophoblast of the human placenta, an organ that is not innervated. We hypothesized that NKB is a paracrine modulator of tone in the fetal placental circulation. We tested this hypothesis using the in vitro perfused human placental cotyledon. Our data show that NKB is a dilator of the fetal vasculature, causing a maximal 25.1 +/- 4.5% (mean +/- SEM; n = 5) decrease in fetal-side arterial hydrostatic pressure (5- microM NKB bolus; effective concentration in the circulation, 1.89 nM) after preconstriction with U-46619. RT-PCR demonstrated the presence of mRNA for NK(1) and NK(2) tachykinin receptors in the placenta. Using selective receptor antagonists, we found that NKB-induced vasodilation is through the NK(1) receptor subtype. We found no evidence for the involvement of either nitric oxide or prostacyclin in this response. This study demonstrates a paracrine role for NKB in the regulation of fetal placental vascular tone.

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“…By using mRNA fingerprinting, increased levels of neurokinin B (NkB) were identified as the promising marker (and potential causative agent) associated with PE [60]. Indeed, significantly higher levels of NkB in the maternal and umbilical cord blood were observed in preeclampsia, compared to pregnancies without complications [61].…”

Section: Etiology and Pathogenesis Of Pementioning

confidence: 99%

Protein Misfolding during Pregnancy: New Approaches to Preeclampsia Diagnostics

Gerasimova

Fedotov

Kachkin

et al. 2019

IJMS

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Preeclampsia (PE) is a multisystem heterogeneous complication of pregnancy remaining a leading cause of maternal and perinatal morbidity and mortality over the world. PE has a large spectrum of clinical features and symptoms, which make diagnosis challenging. Despite a long period of studying, PE etiology is still unclear and there are no reliable rapid tests for early diagnosis of this disease. During the last decade, it was shown that proteins misfolding and aggregation are associated with PE. Several proteins, including amyloid beta peptide, transthyretin, alpha-1 antitrypsin, albumin, IgG k-free light chains, and ceruloplasmin are dysregulated in PE, resulting in toxic deposition of amyloid-like aggregates in the placenta and body fluids. It is also possible that aggregated proteins induce defective trophoblast invasion, placental ischemia, ER stress, and promote PE manifestation. The fact that protein aggregation is an emerging biomarker of PE provides an opportunity to develop new diagnostic approaches based on amyloids special features, such as Congo red (CR) staining and thioflavin T (ThT) enhanced fluorescence.

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The Development of a Genetic Profile of Placental Gene Expression during the First Trimester of Pregnancy: A Potential Tool for Identifying Novel Secreted Markers

Cited by 15 publications

References 17 publications

Accurate and Robust Quantification of Circulating Fetal and Total DNA in Maternal Plasma from 5 to 41 Weeks of Gestation

Accurate and Robust Quantification of Circulating Fetal and Total DNA in Maternal Plasma from 5 to 41 Weeks of Gestation

Neurokinin B Is a Paracrine Vasodilator in the Human Fetal Placental Circulation

Protein Misfolding during Pregnancy: New Approaches to Preeclampsia Diagnostics

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