1984
DOI: 10.1007/bf02134004
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The development and validation of a radioimmunoassay for human Pregnancy-Associated Plasma Protein A (PAPP-A)

Abstract: A specific and sensitive radioimmunoassay for human pregnancy-associated plasma protein A (PAPP-A) is described. This assay utilized tracer repurified by affinity chromatography on heparin-Sepharose. The most sensitive assay developed had a detection limit of 5 microliters PAPP-A in serum, and this protein could not be detected in sera from non-pregnant subjects. During pregnancy, mean circulating levels rose from 0.6 mg/l at 6 weeks gestation to 110 mg/l at term, and PAPP-A was detected in 44 of 101 umbilical… Show more

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Cited by 14 publications
(4 citation statements)
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“…PAPP-A and SP-1 were quantified by radioimmu noassay as described previously [4,5] and hCG mea sured using a commercial kit (Amcrlex M hCG R1A kit, Amersham, UK).…”
Section: Methodsmentioning
confidence: 99%
“…PAPP-A and SP-1 were quantified by radioimmu noassay as described previously [4,5] and hCG mea sured using a commercial kit (Amcrlex M hCG R1A kit, Amersham, UK).…”
Section: Methodsmentioning
confidence: 99%
“…Serum was separated within 2 h of collection, aliquoted and stored at -70°C until assayed. PAPP-A was measured by radioimmunoassay as described previously (Pinto-Furtado et al 1984).…”
Section: Subjects and Blood Samplesmentioning
confidence: 99%
“…Finally, although there is general agreement that PAPP-A-like material has been identified in pre-ovulatory follicular fluid and seminal plasma (Bischof et al 1983a, b;Sinosich et al 1984;Sjoberg et al 1984Sjoberg et al , 1985Stanger et al 1985;Westergaard et al 19851, evidence for the presence of PAPP-A in the peripheral blood in the non-pregnant state is not in such accord, the majority of centres having identified circulating PAPP-A 4 weeks after conception (Anthony et al 1983;Pinto-Furtado et al 1984;Sinosich et al 1982b;Sjoberg et al 1984;Westergaard et al 1983).…”
mentioning
confidence: 99%
“…The coefficient of variation between assays varied from 6.1% down to 3.1% in the same range. PAPP-A estimations were performed by radioimmunoassay described in detail elsewhere (Sinosich et al 1982: Pinto-Furtado et al 1984. The minimum detection limit was 5.0 pgil.…”
Section: Radioirnmunoassaymentioning
confidence: 99%