The development and validation of a radioimmunoassay for human Pregnancy-Associated Plasma Protein A (PAPP-A)

Furtado, Louis; Bolton, A.E.; Grudzinskas, J.G.; Chapman, Michael; Sinosich, Michael J.; Sharma, Vinay

doi:10.1007/bf02134004

Cited by 14 publications

(4 citation statements)

References 18 publications

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“…PAPP-A and SP-1 were quantified by radioimmu noassay as described previously [4,5] and hCG mea sured using a commercial kit (Amcrlex M hCG R1A kit, Amersham, UK).…”

Section: Methodsmentioning

confidence: 99%

Placental Proteins in Peripheral Blood and Tissues of Ectopic Pregnancies

Tornehave

Chemnitz

Westergaard

et al. 1987

Gynecol Obstet Invest

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Circulating human chorionic gonadotrophin (hCG), Schwangerschaftsprotein 1 (SP-1) and pregnancy-associated plasma protein A (PAPP-A) were examined in 10 women with ectopic gestation in relation to distribution and intensity of staining for these molecules using immunohistochemical techniques applied to matched trophoblastic and decidualized endometrial tissues. All 10 women revealed strong staining for hCG and SP-1 in the syncytiotrophoblast, which was apparently unrelated to maternal levels of hCG and SP-1, levels being less than the 10th centile of the normal range in 9/10 and 7/10 women, respectively. By contrast maternal PAPP-A levels seemed to correlate with the intensity and the distribution of staining for PAPP-A. Circulating PAPP-A was undetectable ( < 5 mIU/l) in 4 patients, and below the 10th centile in the remaining 6. Using immunohistochemical techniques hCG, SP-1 and PAPP-A could not be demonstrated in any of the decidualized endometrial tissues studied.

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“…PAPP-A and SP-1 were quantified by radioimmu noassay as described previously [4,5] and hCG mea sured using a commercial kit (Amcrlex M hCG R1A kit, Amersham, UK).…”

Section: Methodsmentioning

confidence: 99%

Placental Proteins in Peripheral Blood and Tissues of Ectopic Pregnancies

Tornehave

Chemnitz

Westergaard

et al. 1987

Gynecol Obstet Invest

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“…Serum was separated within 2 h of collection, aliquoted and stored at -70°C until assayed. PAPP-A was measured by radioimmunoassay as described previously (Pinto-Furtado et al 1984).…”

Section: Subjects and Blood Samplesmentioning

confidence: 99%

“…Finally, although there is general agreement that PAPP-A-like material has been identified in pre-ovulatory follicular fluid and seminal plasma (Bischof et al 1983a, b;Sinosich et al 1984;Sjoberg et al 1984Sjoberg et al , 1985Stanger et al 1985;Westergaard et al 19851, evidence for the presence of PAPP-A in the peripheral blood in the non-pregnant state is not in such accord, the majority of centres having identified circulating PAPP-A 4 weeks after conception (Anthony et al 1983;Pinto-Furtado et al 1984;Sinosich et al 1982b;Sjoberg et al 1984;Westergaard et al 1983).…”

mentioning

confidence: 99%

Comparison of different antibody preparations against pregnancy‐associated plasma protein‐A (PAPP‐A) for use in localization and immunoassay studies

Chemnitz¹,

Folkersen²,

Teisner³

et al. 1986

BJOG

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Summary. Four antibody preparations against pregnancy‐associated plasma protein (PAPP‐A) were compared in order to find an explanation for the contradictory results published on tissue localization, clinical usefulness and biological function of PAPP‐A. One of the preparations studied was a rabbit anti‐PAPP‐A antiserum which has been offered for general scientific use (Bischof et al. 1979). Only the IgG fraction of anti‐PAPP‐A antisera which appeared to be monospecific and had been further absorbed with fetal connective tissue gave specific uniform staining of the cytoplasm of the syncytiotrophoblast exclusively. Circulating PAPP‐A could not be detected by RIA employing this IgG preparation in the non‐pregnant state, or before 18 days after conception. Circulating PAPP‐A could be detected in all seven pregnant women studied within 4 weeks after conception. Identical results were obtained with a commercially available IgG fraction against PAPP‐A.

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“…The coefficient of variation between assays varied from 6.1% down to 3.1% in the same range. PAPP-A estimations were performed by radioimmunoassay described in detail elsewhere (Sinosich et al 1982: Pinto-Furtado et al 1984. The minimum detection limit was 5.0 pgil.…”

Section: Radioirnmunoassaymentioning

confidence: 99%