The Development and Standardization of Testing Methods for Genetically Modified Organisms and their Derived Products^F

Abstract

As the worldwide commercialization of genetically modified organisms (GMOs) increases and consumers concern the safety of GMOs, many countries and regions are issuing labeling regulations on GMOs and their products. Analytical methods and their standardization for GM ingredients in foods and feed are essential for the implementation of labeling regulations. To date, the GMO testing methods are mainly based on the inserted DNA sequences and newly produced proteins in GMOs. This paper presents an overview of GMO testing methods as well as their standardization.Key words DNA; genetically modified organisms; protein; standardization; testing methods Zhang D, Guo J (2011) The development and standardization of testing methods for genetically modified organisms and their derived products.

Qualitative and quantitative PCR detection of insect-resistant genetically modified rice Agb0101 developed in korea

“…최근 정확한 GMO 의 정량분석을 위해서 real-time PCR이 이용되고 있으며, 정량 표준물질로 주로 내재유전자 및 도입유전자의 단편 을 포함한 plasmid을 사용하고 있다. 표준 plasmid의 이용은 목표 단편들에 대하여 정확한 농도로 표준용액을 조제할 수 있기 때문에 이로부터 GMO 시료의 농도를 용이하게 산출할 수 있는 장점이 있다 (Kuribara et al 2002;Toyota et al 2006;Zhang and Guo 2011). 따라서 Agb0101 벼의 정량 분석용 pRBECrR을 제작하였고, 이의 목적하는 내재유전 자 및 도입유전자의 단편들이 벡터 내에 삽입되어 있는 지 염기서열 분석을 수행한 결과, 예상한 염기서열과 정 확하게 일치하는 237 bp의 단편을 확인 하였다 (Fig.…”

Prevalence of genetically modified foods (GM foods) in the United Arab Emirates

“…Most notably, nucleic acid based real time PCR assays are applied globally for qualitative and quantitative detection of GM foods (Dinon, de Melo, & Arisi, 2008;Hardegger, Brodmann, & Hermann, 1999;Holst-Jensen, Ronning, Lovseth, & Berdal, 2003;Vaitilingom, Pijnenburg, Gendre, & Brignon, 1999). The analytical techniques whether protein based or nucleic acid based, complement or overpower each other in certain cases (Zhang & Guo, 2011). Nevertheless, both techniques are currently in use for the detection of genetically modified organisms in food.…”

Loop-Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives

“…In addition, LFS detects only the presence of the expressed exogenous protein, therefore, it can not distinct samples with same exogenous proteins and serves only for qualitative screening. Even though, low sensitivity due to sample matrix effect, low specificity due to the lack of suitable antibody and false positive due to the non-specific binding often affect the detection result [5]. As compared with protein-based methods, DNA-based methods, such as PCR or real-time quantitative PCR (RT-qPCR), are the most widely used analytical approaches in GMO detection due to their high accuracy, reliability, sensitivity, and re-productivity [3] [5] [10] [11].…”