The deubiquitylating enzyme, Cops6, regulates different developmental processes during early zebrafish embryogenesis

Tse, William Ka Fai; You, May Su; Ho, Soo Yei; Jiang, Yun-Jin

doi:10.1387/ijdb.103089wt

Cited by 9 publications

(4 citation statements)

References 17 publications

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“…To understand the mechanism underlying these effects is important because these data could reveal the fundamental cause of the observed effects; further, the data can be utilized to predict and evaluate the impact of in utero EDC exposure. In this study, we performed screening in the early stage of embryogenesis; we selected 3 critical stages, including dorsoventral (DV) patterning (60–75% epiboly), segmentation (8–10 ss), and brain development (prim-5), within 24 hpf (Tse et al, 2009; Tse et al, 2011). …”

Section: Resultsmentioning

confidence: 99%

Early embryogenesis in zebrafish is affected by bisphenol A exposure

et al. 2013

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SummaryExposure of a developing embryo or fetus to endocrine disrupting chemicals (EDCs) has been hypothesized to increase the propensity of an individual to develop a disease or dysfunction in his/her later life. Although it is important to understand the effects of EDCs on early development in animals, sufficient information about these effects is not available thus far. This is probably because of the technical difficulties in tracing the continuous developmental changes at different stages of mammalian embryos. The zebrafish, an excellent model currently used in developmental biology, provides new insights to the field of toxicological studies. We used the standard whole-mount in situ hybridization screening protocol to determine the early developmental defects in zebrafish embryos exposed to the ubiquitous pollutant, bisphenol A (BPA). Three stages (60–75% epiboly, 8–10 somite, and prim-5) were selected for in situ screening of different molecular markers, whereas BPA exposure altered early dorsoventral (DV) patterning, segmentation, and brain development in zebrafish embryos within 24 hours of exposure.

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Section: Resultsmentioning

confidence: 99%

Early embryogenesis in zebrafish is affected by bisphenol A exposure

et al. 2013

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“…PSMD4, functioning as a ubiquitin-degrading enzyme within the proteasome’s 19S regulatory granules, can control numerous biological processes, such as the stability of proteins, the advancement of cancer, and resistance to drugs [ 43 ]. COPS6, a member of the JAMM family, has recently been verified to facilitate tumor advancement and decrease the infiltration of CD8 + T-cells by suppressing the synthesis of IL-6, thus promoting tumor immune escape from cancer [ 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel matrix metalloproteinases-related prognostic signature in hepatocellular carcinoma

Yuan,

Yang,

Gao

et al. 2024

Aging

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Background: Hepatocellular carcinoma (HCC) is the most common primary liver cancer worldwide. Cancer cells’ local infiltration, proliferation, and spread are mainly influenced by the protein hydrolyzing function of different matrix metalloproteinases (MMPs). However, no study has determined the relationship between MMPs and prognostic prediction in HCC. Methods: Expression profiles of mRNA and MMPs-related genes were obtained from publicly available databases. Cox regression and LASSO Cox regression analysis were used to identify and predict MMPs-related prognostic signature and construct predictive models for overall survival (OS). A nomogram was used to validate the accuracy of the prediction model. Drug prediction was performed using the Genomics of Drug Sensitivity in Cancer (GDSC) dataset, and single-cell clustering analysis was performed to further understand the significance of the MMPs-related signature. Results: A MMPs-related prognostic signature (including RNPEPL1, ADAM15, ADAM18, ADAMTS5, CAD, YME1L1, AMZ2, PSMD14, and COPS6) was identified. Using the median value, HCC patients in the high-risk group showed worse OS than those in the low-risk group. Immune microenvironment analysis showed that patients in the high-risk group had higher levels of M0 and M2 macrophages. Drug sensitivity analysis revealed that the IC 50 values of sorafenib, cisplatin, and cytarabine were higher in the high-risk group. Finally, the single-cell cluster analysis results showed that YME1L1 and COPS6 were the major genes expressed in the monocyte cluster. Conclusions: A novel MMPs-related signature can be used to predict the prognosis of HCC. The findings of this research could potentially impact the predictability of the prognosis and treatment of HCC.

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“…Of these five subclasses, the USP family represents the largest subclass of the DUBs (Nijman et al, 2005). Growing evidence demonstrated that deubiquitination plays crucial roles in regulating a wide range of cellular processes and functions, such as signaling pathways (Clague et al, 2012;Eichhorn et al, 2012;Zhang et al, 2012;Zhong et al, 2012), cell cycle (Song and Rape, 2008), protein trafficking (Hasdemir et al, 2009;Clague et al, 2012), DNA repair (Nakada et al, 2010;Clague et al, 2012), transcription (Li et al, 2002;Clague et al, 2012), and early embryogenesis (Tse et al, 2009(Tse et al, , 2011(Tse et al, , 2013. Furthermore, mutations in some DUBs have been associated with diseases (Singhal et al, 2008;Clague et al, 2013), such as ataxin3 (ATXN3) in ataxias (Durcan et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Deubiquitinase Usp18 prevents cellular apoptosis from oxidative stress in liver cells

Lai

Cheung

Tse

2017

Cell Biology International

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Deubiquitinases (DUBs) deconjugate ubiquitin (UBQ) from ubiquitylated substrates to regulate their activity and stability. They play different cellular functions such as cell cycle regulation, DNA repair, and early embryogenesis. Additionally, studies have demonstrated that some DUBs are the signaling targets of cellular stress such as oxidative stress. Reactive oxygen species are generated during normal mitochondrial oxidative metabolism and proper cellular mechanism could protect the cell from the oxidative stress. However, there are limited studies that specifically focus on the role of DUBs in oxidative stress, and thus the underlying protective mechanism by DUBs is not yet known. The report here, for the first time, applied the mouse-specific DUB RT Profiler PCR array to identify DUBs that are responsive to oxidative stress. Out of the tested 83 DUBs, 15 of them were found to be differentially expressed. Among them, Usp18 was found to be induced with a dose- and time-dependent manner of oxidative stress. In functional studies, depletion of Usp18 could stimulate the p53 and caspase 3 protein levels. In addition, knockdown of Usp18 could lead to the reduced cell viability and increased in apoptotic cell death under oxidative stress. Collectively, Usp18 protects the cells from oxidative stress-induced apoptosis which may be through the regulation of p53 and caspase 3.

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The deubiquitylating enzyme, Cops6, regulates different developmental processes during early zebrafish embryogenesis

Cited by 9 publications

References 17 publications

Early embryogenesis in zebrafish is affected by bisphenol A exposure

Early embryogenesis in zebrafish is affected by bisphenol A exposure

Identification of a novel matrix metalloproteinases-related prognostic signature in hepatocellular carcinoma

Deubiquitinase Usp18 prevents cellular apoptosis from oxidative stress in liver cells

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