The Determination of α-Amylase with 2-Chloro-4-nitrophenyl-β-D-maltoheptaoside äs Substrate: Comparison with other Methods

Abstract

Summary:The determination of -amylase with 2-chloro-4-nitrophenyl-ß-Z)-maltoheptaoside äs Substrate was evaluated. The molar lineic absorbance of the liberated product, 2-cliloro-4-nitrophenol, depends on the concentration of the protein present in the test. The catalytic activity concentrations obtained with human native sera, urines and commercial test sera were compared with activity concentrations determined using Substrates with the same carbohydrate chain length. The analytical agreement is acceptable for human sera and urines.