The determination of torasemide and metabolites in plasma by high-performance liquid chromatography

Besenfelder, E

doi:10.1016/0731-7085(87)80029-8

Cited by 18 publications

(18 citation statements)

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“…Urinary excretion of torasemide and its metabolites was measured 0-6, 6-12, 12-24, and 24-48 h after dosing. Concentrations of plasma and urinary torasemide and torasemide metabolites M1, M3, and M5 were measured by means of high-performance liquid chromatography [9]. Non-compartmental kinetic analysis was performed.…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of torasemide and its metabolites in end-stage renal disease

Krämer

Schwab

Braun

et al. 1994

Eur J Clin Pharmacol

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The pharmacokinetics of torasemide, a new loop diuretic, as well as its active metabolites M1 and M3, and its inactive main metabolite, M5, were studied in 12 patients with end-stage renal failure during single i.v. (n = 6) or single oral (n = 6) dosing of 200 mg torasemide, and during chronic oral treatment for 9 days (n = 12). The elimination half-life (t1/2) of torasemide was unchanged in renal failure, whereas t1/2 of the torasemide metabolites M1, M3, and M5 were markedly prolonged. However t1/2 as well as the area under the plasma level time curve of torasemide and its metabolites were unchanged during chronic compared to acute administration. The results of this study suggest that despite the increased half-life of torasemide metabolites M1, M3 and M5 in end-stage renal failure patients, no accumulation of the parent drug torasemide and its metabolites during chronic dosing is demonstrable.

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Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of torasemide and its metabolites in end-stage renal disease

Krämer

Schwab

Braun

et al. 1994

Eur J Clin Pharmacol

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“…[11][12][13]. Determination coefficients for the three responses were: 0.79 (Rs 12 ), 0.99 (Rs 23 ) and 0.97 (Rs 34 ), thus indicating that this model could accurately explain the experimental data.…”

Section: Optimization Designmentioning

confidence: 99%

“…The first developed methods for the determination of Torasemide and its metabolites consisted of a reversed phase chromatographic separation with photometric detection [11][12][13][14][15]. Next, two different voltammetric analytical methods were developed in our laboratory for the analysis of Torasemide [16,17].…”

Section: Introductionmentioning

confidence: 99%

Separation of the high-ceiling diuretic Torasemide and its metabolites by capillary zone electrophoresis with diode-array detection

et al. 2002

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A capillary zone electrophoretic method was developed for the separation of the high-ceiling loop diuretic Torasemide and three of its metabolites (M1, M3 and M5) using an experimental design approach. Two different experimental designs were employed to optimize the developed method: (i) a fractional factorial design examining six factors at two levels (2(6-2)) and (ii) a central composite design examining two factors at two levels (2(2)+2x2+p). The factors studied were: pH, buffer concentration, proportion of boric acid in the mixed boric acid:potassium dihydrogen phosphate background electrolyte, temperature, applied voltage and percentage of organic modifier. Resolution between peaks was established as response. Separation of the four studied compounds was achieved in less than 8 min, using an electrolyte of 20 mM boric acid:potassium dihydrogen phosphate (75:25 v/v) with 15% MeOH adjusted to pH 9.7 with KOH, at a potential of 28 kV. Detection wavelength and temperature were 206 nm and 35 degrees C, respectively.

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“…These include a combined gas chromatographic-mass spectrometric method [4] as well as a few liquid chromatographic separations followed by spectrophotometric [5][6][7] or electrochemical detection [3]. In the methods cited above the metabolites were only partly included.…”

Section: Introductionmentioning

confidence: 99%