The Determination of the Stoichiometry of Cyclodextrin Inclusion Complexes by Spectral Methods: Possibilities and Limitations

Tablet, Cristina; Matei, Iulia; Hillebrand, Mihaela

doi:10.5772/34287

Cited by 16 publications

(11 citation statements)

References 84 publications

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“…The continuous variation method, also known as Job method or Job plot was employed to determine the complex inclusion ratio and was carried out in accordance with the literature (Job, 1928;Tablet, Matei, & Hillebrand, 2012). For this, different volumes of equal molar concentrations of TEL and BCD in phosphate buffer solution pH 7.5 (adjusted with NaOH)…”

Section: Determination Of Inclusion Ratio By Continuous Variation Methodsmentioning

confidence: 99%

“…The continuous variation method was one of the first methods used to determine the inclusion complex stoichiometry experimentally (Job, 1928;Tablet et al, 2012). According with Job's method, the molar fraction of TEL (R) relative to the maximum value of ΔA represents graphically the stoichiometric proportion of investigated inclusion complex.…”

Section: Determination Of Inclusion Ratio By Continuous Variation Metmentioning

confidence: 99%

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The effect of mechanical grinding on the formation, crystalline changes and dissolution behaviour of the inclusion complex of telmisartan and β-cyclodextrins

Borba

Pinotti

Andrade

et al. 2015

Carbohydrate Polymers

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Section: Determination Of Inclusion Ratio By Continuous Variation Methodsmentioning

confidence: 99%

Section: Determination Of Inclusion Ratio By Continuous Variation Metmentioning

confidence: 99%

The effect of mechanical grinding on the formation, crystalline changes and dissolution behaviour of the inclusion complex of telmisartan and β-cyclodextrins

Borba

Pinotti

Andrade

et al. 2015

Carbohydrate Polymers

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“…To determine the stoichiometric ratio between the studied drugs and acriflavine, Job's method of continuous variation was used [29]. Equimolar solutions (8 × 10 −6 M) were prepared from the drug and acriflavine.…”

Section: Determination Of Quenching Mechanism and Stoichiometry Of The Reactionmentioning

confidence: 99%

Studying the quenching resulted from the formation of an association complex between olsalazine or sulfasalazine with acriflavine

Tolba

Elmansi

2021

R. Soc. open sci.

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We report the detection and quantification of important ulcerative colitis drugs olsalazine (OLS) and sulfasalazine (SUL) by the spectrofluorometric method. The proposed method was optimized and validated by using the quenching effect on the acriflavine fluorescence. The method was applied on the detection and quantification of OLS and SUL under optimized conditions showing the calibration curves were linear (range: 1.0–10.0 µg ml −1 ), with correlation coefficients R 2 of 0.9999 for both drugs. The limits of detection (LOD) and quantification (LOQ) were 53 and 104 ng ml −1 for the OLS and 160 and 315 ng ml −1 for the SUL. This method permitted the analysis of OLS and SUL in their pure and pharmaceutical forms. The proposed spectrofluorimetric method was also evaluated against ‘green’ criteria and all the experimental results make it an eco-friendly and safe method for the detection of OLS and SUL.

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“…The resulting solution was then filtered through Whatman filter paper size (0.45µm) and analyzed by UV-Visible spectrophotometer at 324.6nm. 27,28…”

Section: Phase Solubility Studiesmentioning

confidence: 99%

Solubility Enhancement of Embelin by Complexation with Beta Cyclodextrin

Kani¹,

Xavier²

2021

IJPER

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Introduction: Embelin, a phytoconstituent obtained from Embelia ribes of the Myrsinaceae family, has anti-cancer, anti-inflammatory, anti-bacterial, anti-fertility, analgesic, antidiabetic, anti-depressant and wound healing activities. It is hydrophobic in nature leading to low bioavailability. Aim: The present study aims to improve the water solubility and rate of dissolution of Embelin by complexation with β-cyclodextrin. Methods: Inclusion complexes were prepared by physical mixture, kneading and co-precipitation methods. Characterization of complexes was carried out by Fourier-Transform Infrared (FT-IR) spectroscopy and in vitro dissolution study. Differential scanning calorimetry (DSC) and Scanning electron microscopy (SEM) was used to analyze the prepared complexes prepared by the co-precipitation method. Antimicrobial studies of complexes against Staphylococcus aureus and Escherichia coli were carried out by colony counting method. Results: Phase solubility study showed Embelin forms complex with β-cyclodextrin in the ratio 1:2. FT-IR studies of complexes confirmed Embelin forms complex with β-cyclodextrin. DSC and SEM also confirmed the formation of a complex of Embelin with β-cyclodextrin. In vitro dissolution studies showed that the time to release 50 % (t50) of Embelin was in the order 15 min, 30 min, 60 min for complexes prepared by coprecipitation, kneading method and physical mixture respectively. Complexes prepared by the coprecipitation method showed 2 log reductions in the number of S. aureus and 1 log reduction in the number of E. coli in comparison with Embelin. Conclusion: Complexes of Embelin prepared by co-precipitation method resulted in largest percent drug content, enhanced aqueous solubility and antibacterial activity.

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The Determination of the Stoichiometry of Cyclodextrin Inclusion Complexes by Spectral Methods: Possibilities and Limitations

Cited by 16 publications

References 84 publications

The effect of mechanical grinding on the formation, crystalline changes and dissolution behaviour of the inclusion complex of telmisartan and β-cyclodextrins

The effect of mechanical grinding on the formation, crystalline changes and dissolution behaviour of the inclusion complex of telmisartan and β-cyclodextrins

Studying the quenching resulted from the formation of an association complex between olsalazine or sulfasalazine with acriflavine

Solubility Enhancement of Embelin by Complexation with Beta Cyclodextrin

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