The Determination of the Gases of the Blood

Slyke, Donald D. Van; Stadie, William C.

doi:10.1016/s0021-9258(18)85987-7

Cited by 147 publications

(11 citation statements)

References 10 publications

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“…The determination of dissolved oxygen in sea water is fully described by Jacobsen (1921). The determination of carbon dioxide in the water may be made by the Van Slyke manometric method which will be found in the articles by Van Slyke and Stadie (1921), Van Slyke (1927), andVan Slyke andSendroy (1927). An alternative method for determining CO 2 in sea water and brackish waters which utilizes pH determinations is given by Bruce (1924).…”

Section: Methods Finally Adopted For Fishes and Other Macroscopic Aqu...mentioning

confidence: 99%

The Measurement of the Respiratory Exchange of Aquatic Animals

Keys¹

1930

The Biological Bulletin

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Section: Methods Finally Adopted For Fishes and Other Macroscopic Aqu...mentioning

confidence: 99%

The Measurement of the Respiratory Exchange of Aquatic Animals

Keys¹

1930

The Biological Bulletin

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“…The oxygen saturation of arterial blood was determined on samples collected under oil from the femoral artery. All oxygen determinations were made by Van Slyke's technique (1). The non-protein nitrogen was determined by the method of Folin and Wu (2), and the chlorides on the tungstic acid filtrate after the method suggested by Gettler (3).…”

Section: Methodmentioning

confidence: 99%

The Oxygen Content of the Venous Blood of the Dog After Upper Gastrointestinal Tract Obstruction

Haden

Orr

1927

Journal of Experimental Medicine

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“…The overpressure exerted by the released gas was then determined by recording the displacement of mercury in the classical manometer. 4 Typically, the volume of the sample was in the order of a few milliliters. Due to the pressing need to analyze small volumes of samples and to reduce the expenditure of expensive chemicals, efforts were made to miniaturize gasometric measurements.…”

mentioning

confidence: 99%

“…In the early version of the Van Slyke apparatus, gas was liberated from liquid samples by mechanically vibrating the sample vessel and collected above the sample. The overpressure exerted by the released gas was then determined by recording the displacement of mercury in the classical manometer . Typically, the volume of the sample was in the order of a few milliliters.…”

mentioning

confidence: 99%

In-Oleo Microgasometry of Nanoliter-Scale Gas Volumes with Image-Based Detection

Prabhu

Urban

2016

Anal. Chem.

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Gasometric assays involve measurements of the amounts of gases that are released during physical or chemical processes. The available instrumentation for gasometric analysis is generally difficult to use and requires large sample volumes. In some cases, toxic materials (mercury) are involved in the analysis process. Here, we propose a microscale gasometric assay using silicone oil as matrix. Microliter-volume (∼2.5 μL) aqueous droplets, containing sample and reagent and/or catalyst, are introduced to the oil matrix and merged. Nanoliters of gaseous products are released to the surrounding oil matrix forming tiny spherical bubbles. Due to the huge differences between refractive indices of the released gas and the surrounding liquids (aqueous assay solution, oil), the gas bubbles are clearly visible from the top, when the assay reservoir is illuminated from the bottom with light-emitting diodes. The released gas bubbles are documented by recording videos of the assay reservoir. Individual frames within these videos are then analyzed by a graphical software to obtain diameters of every gas bubble at each time point. Following a fixed period of time (typically, 5-90 s) after the sample/reagent droplet merger, the volume of the released gas scales with the amount of the substrate (analyte) present in the sample droplet. For example, hydrogen peroxide can be decomposed to oxygen by 0.44 U catalase enzyme and semiquantified in the range up to ∼1.0 μmol. Glutathione can be detected in a two-step procedure ((1) oxidation of glutathione by hydrogen peroxide; (2) decomposition of the hydrogen peroxide residue by catalase).

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The Determination of the Gases of the Blood

Cited by 147 publications

References 10 publications

The Measurement of the Respiratory Exchange of Aquatic Animals

The Measurement of the Respiratory Exchange of Aquatic Animals

The Oxygen Content of the Venous Blood of the Dog After Upper Gastrointestinal Tract Obstruction

In-Oleo Microgasometry of Nanoliter-Scale Gas Volumes with Image-Based Detection

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