The determination of the D/H ratio of non-exchangeable hydrogen in cellulose extracted from aquatic and land plants

Epstein, Samuel; Yapp, Crayton J.; Hall, John S.

doi:10.1016/0012-821x(76)90251-x

Cited by 258 publications

(121 citation statements)

References 11 publications

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“…In general however, C-bound H as in the DMS-moiety of DMSP, are considered nonexchangeable (Epstein et al 1976). Although heterotrophic reprocessing of photosynthate in Lemna gibba did result in exchange of part of the C-bound H with water (Yakir and DeNiro 1990), this was not observed in algae (Estep and Hoering 1980;Estep 1981).…”

Section: Discussionmentioning

confidence: 99%

In vivo DMSP‐biosynthesis measurements using stable‐isotope incorporation and proton‐transfer‐reaction mass spectrometry (PTR‐MS)

Stefels

Dacey

Elzenga

2009

Limnology & Ocean Methods

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Assessing turnover rates of dimethylsulfoniopropionate (DMSP), precursor of the climate-active gas dimethylsulfide (DMS), under different environmental conditions is fundamental to accurately modeling the marine sulfur cycle. Current gas chromatographic methods only provide net results of all processes acting on the different sulfur pools and are unable to measure fluxes, leaving room for speculation on the controlling factors and magnitudes of turnover processes. Here we present a new method in which stable isotope additions (D 2 O or NaH 13 CO 3 ) are used to follow the in vivo production of DMSP. Incorporation of deuterium or 13 C into DMSP was monitored using a proton-reaction-transfer mass spectrometer (PTR-MS). A growth model was developed to calculate specific DMSP synthesis rates from the progress in mass ratio of labeled versus nonlabeled DMSP over time. Instead of measuring the amount of incorporated isotope, our method uses ratios of molecule masses. This approach has the advantage that the effect of the isotope addition on the change in mass ratio is amplified by the number of positions the label can occupy within the molecule. Application of the method was demonstrated in cultures of the ubiquitous DMSP producer Emiliania huxleyi and further developed for use in short-time incubation studies with water from the Sargasso Sea during July 2004. Very low specific synthesis rates between 0.05 and 0.2 d -1 could be measured. The method is the first to measure in vivo DMSP production rates. It is relatively easy to apply, highly sensitive, and may also be applicable to other biogenic compounds.

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Section: Discussionmentioning

confidence: 99%

In vivo DMSP‐biosynthesis measurements using stable‐isotope incorporation and proton‐transfer‐reaction mass spectrometry (PTR‐MS)

Stefels

Dacey

Elzenga

2009

Limnology & Ocean Methods

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“…Lina x H. spontaneum Canada Park (HS92) and their parents, were tested in a fully randomized block design with five replicates of each genotype per treatment, and arranged so that each hydroponics tub contained one replicate of each genotype. On 11 August 1995, germinated and vernalised plants were put into a continuously aerated hydroponics solution (Hewitt, 1966), with additional sodium silicate (Epstein, Yapp & Hall, 1976), and mixed in bulk before each weekly change. Nitrogen (5 mol m"^ N) was supplied as Ca(NO3)2 and KNOg, always from the same reagent bottles, reserved for this experiment alone.…”

Section: Methodsmentioning

confidence: 99%

Correlating molecular markers with physiological expression in Hordeum, a developing approach using stable isotopes

Handley¹,

Robinson²,

Scrimgeour³

et al. 1997

New Phytologist

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SUMMARYThis paper describes a developing approach for correlating molecular markers with phenotypic expression in barley, and research examples are given. This approach is intended to elucidate the connection between defined parts of the barley genome and their physiological expressions. To illustrate our approach (up to the point of marker correlation), the materials, methods and results of an experiment are presented. Briefly, the natural abundance levels of carbon and nitrogen isotopes {S^^C and ^^^N) serve as the primary screening tools for assessing barley genotypes for their responses to imposed abiotic stresses. The isotopic responses are then correlated with molecular markers as the first step in identifying the mechanisms relating genetic variation with phenotypic expression.A well-described mechanistic model exists for interpreting plant ^^^C, and this enables partitioning of the physiological processes contributing to the whole plant 8^^C Although plant 5^^N appears to be equally well correlated with genotype and environment, there is no mechanistic model explaining this isotopic signature. Hence, (^^^N is used empirically and in parallel with attempts to understand its underlying mechanism(s).

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“…2). Within the group of plants with 6'3C values ranging from -30%o to -25%o, there is a broad overlap in the oxygen isotope ratio among C3 plants having 680 Ziegler et al (25) proposed that CAM plants are enriched in deuterium relative to C3 and C4 plants because of their ability to maintain metabolic activity under water stress. They argued that, under water stress, plant water becomes enriched in deuterium during evaotranspiration (23), and this enrichment is passed on to the organically bound hydrogen.…”

Section: Methodsmentioning

confidence: 99%

Carbon, Hydrogen, and Oxygen Isotope Ratios of Cellulose from Plants Having Intermediary Photosynthetic Modes

Sternberg¹,

DeNiro²,

Ting³

1984

Plant Physiol.

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Carbon and hydrogen isotope ratios of cellulose nitrate and oxygen isotope ratios of cellulose from species of greenhouse plants having different photosynthetic modes were determined. When hydrogen isotope ratios are plotted against carbon isotope ratios, four clusters of points are discernible, each representing different photosynthetic modes: C3 plants, C4 plants, CAM plants, and C3 plants that can shift to CAM or show the phenomenon referred to as CAM-cycling. The combination of oxygen and carbon isotope ratios does not distinguish among the different photosynthetic modes. Analysis of the carbon and hydrogen isotope ratios of cellulose nitrate should prove useful for screening different photosynthetic modes in field specimens that grew near one another. This method will be particularly useful for detection of plants which show CAMcycling.There are three major photosynthetic pathways in plants: C3,2 C4, and CAM. Carbohydrates in all three are synthesized by the Calvin cycle. In C3 plants, CO2 is fixed directly into the Calvin cycle, with the primary carboxylation product being a threecarbon compound (11). In C4 and CAM plants, the initial carboxylation product is a four-carbon compound (10,

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The determination of the D/H ratio of non-exchangeable hydrogen in cellulose extracted from aquatic and land plants

Cited by 258 publications

References 11 publications

In vivo DMSP‐biosynthesis measurements using stable‐isotope incorporation and proton‐transfer‐reaction mass spectrometry (PTR‐MS)

In vivo DMSP‐biosynthesis measurements using stable‐isotope incorporation and proton‐transfer‐reaction mass spectrometry (PTR‐MS)

Correlating molecular markers with physiological expression in Hordeum, a developing approach using stable isotopes

Carbon, Hydrogen, and Oxygen Isotope Ratios of Cellulose from Plants Having Intermediary Photosynthetic Modes

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