The parameters of the extraction and hydrolysis of soil carbohydrates by methods involving 24N H,SO, and N H2S04 were studied for a sandy granitic loam. Sugars were measured by alkaline ferricyanide, orcinol, anthrone (hexoses), orcinol-ferric chloride (pentoses), cysteine-sulphuric acid (methyl pentoses), glucose oxidase (glucose), and also by analysis of the individual sugars by paper chromatography.After shaking the soil with z4N H2S04 at zoo C a further period of treatment with N H2S04 at IOOO C was required to obtain maximum hydrolysis of the soil carbohydrates. This period decreased from 17 h to 5 has the time with 24N H,S04 increased from z to 16 h. N H2S04 at 1 0 0 ' C alone was less efficient. The extraction of pentoses by 24N H,SO, reached a maximum within 8 h, and methyl pentoses within 4 h, both declining thereafter. The release of hexoses was continuing after 40 h.The best compromise involved extraction with 24N H,SO, for 16 h followed by hydrolysis with N H,S04 for 5 h. By this treatment yields of pentoses and methyl pentoses were respectively gg and gz per cent of the maximum obtainable.