The determination of nitrate and nitrite in cured meat by HPLC/UV

Dennis, Michael; Key, Pauline; Papworth, T.; Pointer, M.; Massey, Robert C.

doi:10.1080/02652039009373908

Cited by 45 publications

(20 citation statements)

References 8 publications

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“…The influence of nitrite on the determination of nitrate was also examined. Figure 4 shows the cyclic voltammograms of 0.1 M NaNO 3 with and without the presence of 0.1 M NaNO 2 [4,22,23]. Initially, it was thought that increasing the concentration of nitrite led to a decrease in the nitrate peak current at -1.4 V. This corresponds well with previous reports that nitrite can become a substantial interferent when it reaches the same concentration as nitrate [4,22,23,41].…”

Section: The Effect Of Interferent Ions On the Nitrate Peak Currentsupporting

confidence: 66%

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A conducting polymer/Ag nanoparticle composite as a nitrate sensor

Atmeh

Alcock-Earley

2011

J Appl Electrochem

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A novel electrochemical sensor was fabricated by electrodeposition of silver nanoparticles on pre-synthesized polypyrrole (PPy), to form a PPy/Ag composite matrix on a glassy carbon electrode. The electrochemical sensor exhibited strong catalytic activity with regards to nitrate reduction. Scanning electron microscopy and cyclic voltammetry were used for the characterization of the composite. The detection limit of nitrate was found to be 5 lM. Moreover, the sensor showed excellent sensitivity, selectivity, and stability.

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Section: The Effect Of Interferent Ions On the Nitrate Peak Currentsupporting

confidence: 66%

“…A voltammetric method employing a copper-plated glassy carbon electrode is affected by the presence of phosphate ions [21]. In addition several chemical methods have also been investigated [22,23].…”

Section: Introductionmentioning

confidence: 99%

A conducting polymer/Ag nanoparticle composite as a nitrate sensor

Atmeh

Alcock-Earley

2011

J Appl Electrochem

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“…In addition, the Health Department of the Basque Country provided information on the concentration of nitrates in the drinking water as well as results of chemical analyses in different groups of foods for the period from 1990 to 1995. 6 Nitrate and nitrite ions were determined by the rapid strong anion exchange high-performance liquid chromatography/ultraviolet procedure described by Dennis et al 7 This method has a quantification limit of 5 mg/kg with a coefficient of variation of Ͻ5%. Nitrate recovery, calculated from enriched samples, ranged between 98% and 106%.…”

Section: Methodsmentioning

confidence: 99%

Methemoglobinemia and Consumption of Vegetables in Infants

Benito²,

2001

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ABSTRACT. Objective. To assess clinical and epidemiologic data of 7 infants diagnosed with acquired methemoglobinemia at the pediatric emergency department between 1993 and 1998. All cases were attributed to the consumption of mixed vegetables.Methods. Medical records were reviewed to collect anamnestic data; history of food ingestion; and results of physical examination, pulse oximetry, gasometry, cooximetry, urinalysis, and outcome. Local health authorities provided information on nitrate concentration in running water and in vegetables of common consumption in the area.Results. The mean age of the patients was 8.14 months (range: 7-13). None of the infants was undernourished, had diarrhea, or was given any drug. Drinking water showed a nitrate concentration of 3 to 6 ppm. All were fed homemade purée of mixed vegetables, prepared in advance and kept in the refrigerator for 12 to 27 hours. Silver beets were a common ingredient. No case showed metabolic acidosis. Methemoglobin level ranged between 10% and 58%. Three cases had nitrituria. Silver beets in our area were the vegetables with the highest nitrate concentration (mean: 3200 mg/kg).Conclusions. Consumption of silver beets and incorrect storage of homemade purées of mixed vegetables were potential causes of methemoglobinemia in this series. The disease may occur in children older than 6 months of age. Nitrituria in a cyanotic infant may suggest the diagnosis of methemoglobinemia. Pediatrics 2001; 107:1024 -1028; methemoglobinemia, nitrates, nitrites, food analysis, infant food, food preservation.ABBREVIATIONS. Sao 2 , oxygen saturation; Hb, hemoglobin. M ethemoglobinemia is a rare cause of cyanosis in pediatric patients. Methemoglobin, a derivative of hemoglobin in which the iron component has been oxidized from the Fe 2ϩ to the Fe 3ϩ state, imparts a characteristic brownish color to blood and is unable to transport oxygen. 1,2 Under physiologic conditions, methemoglobin reduction is accomplished primarily by red cell reduced nicotinamide adenine dinucleotide reductase so efficiently that there is Ͻ1% of methemoglobin in the circulating blood. Hereditary methemoglobinemia is attributable either to deficiency of erythrocyte methemoglobin reductase or to the presence of one of the M hemoglobins. Acquired methemoglobinemia is induced by oxidizing agents, particularly chlorates and inorganic and organic nitrites, or by exposure to certain drugs or their metabolites.The most common sources of nitrate exposure are well water, which is mixed with infant formula, consumption of some vegetables with high nitrate concentration, administration of oxidant drugs, and the presence of diarrheal episodes. After ingestion, nitrates are converted to nitrites by fecal organisms, rapidly absorbed from the intestine by passive diffusion reaching the systemic circulation without undergoing first-pass metabolism in the liver (extrahepatic metabolism). Nitrites are potent oxidant agents of ferrohemoglobin. Infants younger than 6 months old are particularly susceptible to nitrate-...

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“…3 Although nitrate has more stability and less toxicity than nitrite, this compound is also of concern because of its easy biodegradation and conversion to nitrite by microbial reduction in food products. 4,5 For these reasons, a rapid and sensitive method for determining low levels of nitrite and nitrate directly from aqueous samples is desirable.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Nitrite and Nitrate in Potato and Water Samples Using Negative Electrospray Ionization Ion Mobility Spectrometry

2012

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Nowadays, nitrite and nitrate ions are analyzed in biological samples using laborious and expensive methods; such as HPLC, CE, MS-MS. In this work, the simultaneous analysis of nitrite and nitrate ions was conducted by electrospray ionization-ion mobility spectrometry (ESI-IMS), without using any complicated or laborious derivitization step. Ion mobility spectrometry with low cost, inexpensive maintenance and very fast analysis makes an attractive technique for the simultaneous determination of these ions in foodstuff and drinking water samples. The analyte interference was systematically investigated for binary mixture analysis. The obtained results provided detection limits of 3.8 and 4.7 μg/L for nitrite and nitrate, respectively. A linear dynamic range of about 2 orders of magnitude, and relative standard deviations below 5% were obtained by the proposed method for the analysis of both ions. Also, the proposed method was used to analyze various real samples of potato and drinking water samples, and the obtained results confirmed the capability of negative ESI-IMS for the simultaneous detection of nitrite and nitrate.

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The determination of nitrate and nitrite in cured meat by HPLC/UV

Cited by 45 publications

References 8 publications

A conducting polymer/Ag nanoparticle composite as a nitrate sensor

A conducting polymer/Ag nanoparticle composite as a nitrate sensor

Methemoglobinemia and Consumption of Vegetables in Infants

Simultaneous Determination of Nitrite and Nitrate in Potato and Water Samples Using Negative Electrospray Ionization Ion Mobility Spectrometry

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