The determination of monosaccharide in different years Qingzhuan Dark Tea polysaccharide by liquid chromatography–mass spectrometry

Abstract

Aim To establish a fast, sensitive and accurate high‐performance liquid chromatography‐tandem mass spectrometry (HPLC‐MS/MS) method for determining the monosaccharide content of Qingzhuan Dark Tea polysaccharides in different years (2 years, 5 years and 11 years). Methods The optimised chromatographic conditions were achieved on a C18 column (5.0 μm, 250 mm × 4.6 mm inner diameter). The mobile phase flow rate was 0.9 mL/min and the column temperature was set to 27°C. The aqueous phase A (5 mM aqueous ammonium acetate) and organic phase B (acetonitrile) were used to elute the target analyses isocratically (0–60 min: 18% B). The mass spectrometer detector was equipped with an electron spray ionisation (ESI)source, and multiple reaction monitoring (MRM) mode was used for the determination of 1‐phenyl‐3‐methyl‐5‐pyrazolone (PMP) derived monosaccharides. Results We carried out a comprehensive methodological validation of PMP derived monosaccharides, including linearity, precision, stability and repeatability. Nine monosaccharides (rhamnose, mannose, ribose, glucose, galacturonic acid, xylose, galactose, fucose and arabinose) of Qingzhuan Dark Tea polysaccharides were identified, in which ribose and fucose were reported for the first time. The results showed the contents of these nine monosaccharides differed significantly among different years. Conclusions The validated method is reliable, accurate, repeatable and can be applied to quality assessment of these monosaccharides.

A Rising Star in Dark Teas: The Processing, Microorganisms, Chemicals, Bioactivities, and Safety of Qingzhuan Tea

“…Qingzhuan tea polysaccharides are acid polysaccharide or acidic glycoprotein binding to protein, mainly composed of arabinose, rhamnose, ribose, fucose, galactose, glucose, xylose, mannose, and galacturonic acid. , During the process of fermentation, the tea polysaccharides showed a trend of first rising and then decreasing due to the change in microbial population. Thermophilic strains can produce cellulase and amylase to increase the content of soluble sugar. , Among them, cellulase can degrade cellulose to produce glucose, and amylase can hydrolyze starch to produce oligosaccharides.…”

Fingerprinting Evaluation and Gut Microbiota Regulation of Polysaccharides from Jujube (Ziziphus jujuba Mill.) Fruit

“…Monosaccharides could not be directly detected due to their strong polarity, poor absorption, and low ionization efficiency. PMP derivatization was beneficial for the detection of multifarious monosaccharides because of the advantages of derivative stability, moderate derivative condition, strong ultraviolet absorption, and easy ionization [ 23 ]. Usually, HPLC/UHPLC-UV or LC-MS/MS was used for qualitative and quantitative analysis of PMP derivatization monosaccharides [ 24 , 25 ].…”