The determination of minimum inhibitory concentrations of selected antimicrobials for porcine Haemophilus parasuis isolates from the Czech Republic

Nedbalcová, Kateřína; Zouharová, Monika; Šperling, Daniel

doi:10.2754/avb201786020175

Cited by 7 publications

(9 citation statements)

References 21 publications

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“…Serotypes 5 and 4 are dominant in China [3]. With the abuse of antibiotics, antimicrobial-resistant G. parasuis emerge in different degrees, which bring serious threat to the global economy and public health [4].…”

Section: Introductionmentioning

confidence: 99%

Exploration of Clinical Breakpoint of Danofloxacin for Glaesserella parasuis in Plasma and in PELF

Huang

Luo

et al. 2021

Antibiotics

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Background: In order to establish the clinical breakpoint (CBP) of danofloxacin against G. parasuis, three cutoff values, including epidemiological cutoff value (ECV), pharmacokinetic-pharmacodynamic (PK-PD) cutoff value (COPD) and clinical cutoff value (COCL), were obtained in the present study. Methods: The ECV was calculated using ECOFFinder base on the MIC distribution of danfloxacin against 347 G. parasuis collected from disease pigs. The COPD was established based on in vivo and ex vivo PK-PD modeling of danofloxacin both in plasma and pulmonary epithelial lining fluid (PELF) using Hill formula and Monte Carlo analysis. The COCL was established based on the relationship between the possibility of cure (POC) and MIC in the clinical trials using the “WindoW” approach, nonlinear regression and CART analysis. Results: The MIC50 and MIC90 of danofloxacin against 347 G. parasuis were 2 μg/mL and 8 μg/mL, respectively. The ECV value was set to 8 μg/mL using ECOFFinder. Concentration-time curves of danofloxacin were fitted with a two-compartment PK model. The PK parameters of the maximum concentration (Cmax) and area under concentration-time curves (AUC) in PELF were 3.67 ± 0.25 μg/mL and 24.28 ± 2.70 h·μg/mL, higher than those in plasma (0.67 ± 0.01 μg/mL and 4.47 ± 0.51 h·μg/mL). The peak time (Tmax) in plasma was 0.23 ± 0.07 h, shorter than that in PELF (1.61 ± 0.15 h). The COPD in plasma and PELF were 0.125 μg/mL and 0.5 μg/mL, respectively. The COCL calculated by WindoW approach, nonlinear regression and CART analysis were 0.125–4 μg/mL, 0.428 μg/mL and 0.56 μg/mL, respectively. The 0.5 μg/mL was selected as eligible COCL. The ECV is much higher than the COPD and COCL, and the clinical breakpoint based on data in plasma was largely different from that of PELF. Conclusions: Our study firstly established three cutoff values of danofloxacin against G. parasuis. It suggested that non-wild-type danofloxacin-resistant G. parasuis may lead to ineffective treatment by danofloxacin.

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Section: Introductionmentioning

confidence: 99%

Exploration of Clinical Breakpoint of Danofloxacin for Glaesserella parasuis in Plasma and in PELF

Huang

Luo

et al. 2021

Antibiotics

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“…For macrolides, two studies from Europe reported data for tulathromycin . One of them, with data from Denmark, France and Germany, found full susceptibility among 68 isolates (El Garch et al., 2016 ), and the other with data from the Czechia reported 13.3% of 30 isolates resistant (Nedbalcová et al., 2017 ). Three studies reported data for erythromycin with resistance proportions ranging from 1.8% to 50%.…”

Section: Assessmentmentioning

confidence: 99%

“…There are no internationally recognised CBP for G. parasuis , but at least three of the studies from which data were extracted (Zhou et al., 2010 ; El Garch et al., 2016 ; Nedbalcová et al., 2017 ) had adapted the CLSI breakpoints available for A. pleuropneumoniae and/or P. multocida . Some other studies also referred to CLSI breakpoints, but for those it was unclear if they had used the same approach (e.g.…”

Section: Assessmentmentioning

confidence: 99%

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Assessment of animal diseases caused by bacteria resistant to antimicrobials: Swine

Nielsen¹,

Bicout²,

Calistri³

et al. 2021

EFS2

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In this opinion, the antimicrobial‐resistant bacteria responsible for transmissible diseases that constitute a threat to the health of pigs have been assessed. The assessment has been performed following a methodology based on information collected by an extensive literature review and expert judgement. Details of the methodology used for this assessment are explained in a separate opinion. A global state of play of antimicrobial resistant Escherichia coli, Streptococcus suis, Actinobacillus pleuropneumoniae, Pasteurella multocida, Glaeserella parasuis, Bordetella bronchiseptica, Staphylococcus aureus, Staphylococcus hyicus, Brachyspira hyodysenteriae, Trueperella pyogenes, Erysipelothrix rhusiopathiae, Streptococcus dysgalactiae, Mycoplasma hyosynoviae, Mycoplasma hyorhinis, Mycoplasma hyopneumoniae and Brachyspira pilosicoli has been provided. Among those bacteria, EFSA identified E. coli and B. hyodysenteriae with > 66% certainty as being the most relevant antimicrobial resistant bacteria in the EU based on the available evidence. The animal health impact of these most relevant bacteria, as well as their eligibility for being listed and categorised within the animal health law framework will be assessed in separate scientific opinions.

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“…Based on the results of in vitro susceptibility testing of A. pleuropneumoniae, P. multocida and H. parasuis to antimicrobials, the aminopenicillins (amoxicillin), and fluoroquinolones appear to be suitable for the treatment of infections caused by these pathogens in the field due to the high efficiency and relatively low level of resistance. However, some of the commonly used antimicrobials for treatment of respiratory infections of pigs are becoming less effective due to increased resistance among bacterial pathogens in recent years (Aarestrup et al 2004;Cai et al 2005;De la Fuente et al 2007;Nedbalcova and Kucerova 2013;de Jong et al 2014;Nedbalcova et al 2014;El Garch et al 2016;El Garch et al 2017;Nedbalcova et al 2017;Sweeney et al 2017). The number of effective treatment options is limited, partially due to the recent regulations, such as in the case of third-and fourth-generation cephalosporines, which are coming under more scrutiny due to the associated evolution of extended-spectrum betalactamase resistance genes.…”

mentioning

confidence: 99%

Post-antibiotic effect of marbofloxacin, enrofloxacin and amoxicillin against selected respiratory pathogens of pigs

Nedbalcová¹,

Zouharová²,

Šperling³

2019

Vet. Med. - Czech

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The post-antibiotic effect is defined as the period of bacterial growth suppression that persists after a limited exposure of organisms to antimicrobials and knowledge of its duration is important in establishing and optimising current dosing schedules for the treatment of bacterial infections. The post-antibiotic effect of marbofloxacin, enrofloxacin and amoxicillin were evaluated in vitro for Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida strains which originated from clinical samples of diseased pigs and were confirmed as susceptible to all tested antimicrobials based on determination of minimal inhibitory concentrations. The post-antibiotic effect for individual antimicrobials was monitored at five and ten times minimum inhibitory concentrations for one and two hours. The duration of the post-antibiotic effect for tested antimicrobials was found to exhibit the following order for all tested pathogens: marbofloxacin > enrofloxacin > amoxicillin. The longest duration of post-antibiotic effect of all tested antimicrobials was found in A. pleuropneumoniae and the shortest post-antibiotic effect duration was detected in P. multocida. No statistical differences in post-antibiotic effect duration were found within marbofloxacin and enrofloxacin in A. pleuropneumoniae and H. parasuis strains. In P. multocida strains there was a statistically significant difference (P = 0.0189). On the other hand, the differences between amoxicillin and marbofloxacin or enrofloxacin were statistically significant in all cases (P-values ranged between 0.0058 and 0.008). The prolonged post-antibiotic effect of fluoroquinolones and amoxicillin on important Gram-negative swine pathogens was confirmed. The results can be used to clarify the effect and mechanism of action of antimicrobial drugs in veterinary medicine.

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The determination of minimum inhibitory concentrations of selected antimicrobials for porcine Haemophilus parasuis isolates from the Czech Republic

Cited by 7 publications

References 21 publications

Exploration of Clinical Breakpoint of Danofloxacin for Glaesserella parasuis in Plasma and in PELF

Exploration of Clinical Breakpoint of Danofloxacin for Glaesserella parasuis in Plasma and in PELF

Assessment of animal diseases caused by bacteria resistant to antimicrobials: Swine

Post-antibiotic effect of marbofloxacin, enrofloxacin and amoxicillin against selected respiratory pathogens of pigs

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