The determination of microgram amounts of beryllium using acetyl acetone

Determination of Trace Amounts of Beryllium(II) in Drinking Water and of Beryllium Vapor in Air by Graphite-Furnace Atomic Absorption Spectrophotometry Using Acetylacetone as a Chelating Agent

“…Since the final optimum pH of this study was in the range of 5.5 -6.0, 19,27 we had to pre-adjust the pHs of those acidified samples to about 5.5 -6.0 by NH3(aq). After addition of the Nash reagent (7.0 ml, i.e., 14 mmol ammonium acetate buffer and 14 mg acetylacetone) and a small amount of HCl solution (3.0 ml, 0.2%(v/v)) to unknown samples (80 ml), or appropriate amounts (0 -40.0 ng) of Be(II) standard solutions, each mixture was adjusted to 100 ml with pure water.…”

“…15 Some samples are preconcentrated on a glass-fibre filter, 16 or on an anion-exchange resin, 17 followed by solid-phase spectrophotometry, or preconcentrated by forming a complex with Chromazurol S and measured by spectrophotometry, 18 or preconcentrated by reacting with acetylacetone, being extracted into chloroform, and being measured by UV-spectrophotometry. 19 Others were derivatized with trifluoroacetylacetone to form volatile compounds of beryllium trifluoroacetylacetonate, Be(tfa)2, extracted into benzene, and measured by GC (electron capture detection). [20][21][22] The beryllium and its compounds in air particulates measured by NIOSH 23 was collected on a cellulose ester membrane filter.…”

“…The beryllium content was then determined by GC(ECD) 21 as a volatile derivative of Be(tfa)2. Among several complexing agents for beryllium(II), acetylacetone 2,19 has been commonly used because the over-all formation constant 24,25 of beryllium acetylacetonate, Be(acac)2, is about 10 13.9 . In this paper, we used acetylacetone as a chelating agent for beryllium(II) in drinking water (100 ml) in ammonium acetate buffer, together with a slightly acidic HCl solution at pH 5.5.…”

Application of Acetylacetone Chelation Solid-Phase Extraction to GFAAS Measurements of Trace Amounts of Beryllium in Marine Organisms

“…The dissolved Be(II) in the sample was reacted with acetylacetone (acac) [12][13][14][15][16] in the presence of ammonium acetate buffer (pH = 6.0) to form the chelate of beryllium acetylacetonate [Be(acac)2]. 17 The chelate was preconcentrated on a home-made C18 cartridge, and then eluted with methanol (0.50 ml). Hence, trace amounts of Be (0.046 -2.59 ng) in a marine organism sample (10 mg) can be accurately determined by graphite-furnace atomic absorption spectrophotometry (GFAAS).…”

Determination of Beryllium and Selenium in Human Urine and of Selenium in Human Serum by Graphite-Furnace Atomic Absorption Spectrophotometry

“…After spiking 4.00 and 8.00 ng of Merck Be in 500 µL of urine samples, respectively, the average recoveries from triplicates were 98.3 -103% with RSD within 2.5%. The proposed method uses acetylacetone to react with Be(II) in the presence of ammonium acetate buffer of pH 6.0 to form a chelate of beryllium acetylacetonate, 32,33 which enhances the sensitivity for Be. The proposed method can be applied easily and accurately to the measurement of Be (0.37 -65.0 µg/l) in urine samples.…”