The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid

Woessner, Jeff

doi:10.1016/0003-9861(61)90291-0

Cited by 3,604 publications

(1,745 citation statements)

References 19 publications

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“…Chondroitin sulfate in deionized water was used as standard. Total collagen content was determined by measuring the hydroxyproline content of the constructs after acid hydrolysis and reaction with p-dimethylaminobenzaldehyde and chloramine-T [24]. Dry weights were obtained from all constructs after 48 hours of lyophilization to normalize the measured extracellular matrix components (ECM).…”

Section: Biochemical Analysismentioning

confidence: 99%

Engineering Musculoskeletal Tissues with Human Embryonic Germ Cell Derivatives

et al. 2010

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The cells derived from differentiating embryoid bodies of human embryonic germ (hEG) cells express a broad spectrum of gene markers and have been induced toward ectoand endodermal lineages. We describe here in vitro and in vivo differentiation of hEG-derived cells (LVEC line) toward mesenchymal tissues. The LVEC cells express many surface marker proteins characteristic of mesenchymal stem cells and differentiated into cartilage, bone, and fat. Homogenous hyaline cartilage was generated from cells after 63 population doublings. In vivo results demonstrate cell survival, differentiation, and tissue formation. The high proliferative capacity of hEG-derived cells and their ability to differentiate and form three-dimensional mesenchymal tissues without teratoma formation underscores their significant potential for regenerative medicine. The adopted coculture system also provides new insights into how a microenvironment comprised of extracellular and cellular components may be harnessed to generate hierarchically complex tissues from pluripotent cells. STEM CELLS 2010;28:765-774 Disclosure of potential conflicts of interest is found at the end of this article.

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Section: Biochemical Analysismentioning

confidence: 99%

Engineering Musculoskeletal Tissues with Human Embryonic Germ Cell Derivatives

et al. 2010

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“…Explant digests and conditioned media were spectrophotometrically assayed for sGAG content by the dimethylmethylene blue (DMMB) dye-binding method (Farndale, et al, 1986) and for hydroxyproline content by the chloramine-T/para-dimethylaminobenzadehyde reaction (Woessner, 1961). Collagen content was calculated from the hydroxyproline content assuming a collagen:hydroxyproline mass ratio of 8:1 (Reddy and Enwemeka, 1996).…”

Section: Biochemistrymentioning

confidence: 99%

Selective and non-selective metalloproteinase inhibitors reduce IL-1-induced cartilage degradation and loss of mechanical properties

et al. 2007

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Articular cartilage undergoes matrix degradation and loss of mechanical properties when stimulated with proinflammatory cytokines such as interleukin-1 (IL-1). Aggrecanases and matrix metalloproteinases (MMPs) are thought to be principal downstream effectors of cytokine-induced matrix catabolism, and aggrecanase-or MMP-selective inhibitors reduce or block matrix destruction in several model systems. The objective of this study was to use metalloproteinase inhibitors to perturb IL-1-induced matrix catabolism in bovine cartilage explants and examine their effects on changes in tissue compression and shear properties. Explanted tissue was stimulated with IL-1 for up to 24 days in the absence or presence of inhibitors which were aggrecanase-selective, MMP-selective, or non-selective. Analysis of conditioned media and explant digests revealed that aggrecanase-mediated aggrecanolysis was delayed to varying extents with all inhibitor treatments, but that aggrecan release persisted. Collagen degradation was abrogated by MMP-and non-selective inhibitors and reduced by the aggrecanase inhibitor. The inhibitors delayed but did not reduce loss of the equilibrium compression modulus, whereas the loss of dynamic compression and shear moduli was delayed and reduced. The data suggest that non-metalloproteinase mechanisms participate in IL-1-induced matrix degradation and loss of tissue material properties.

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“…Liver samples were freeze-dried and hydrolyzed by 6 N HCl at 108°C for 24 h. One part of the hydrolysates was used for hydroxyproline measurement by a colorimetric method (Woessner, 1961). Collagen content, expressed as g/100 g of liver (dry weight), was calculated by taking into account that collagen contains 13% of hydroxyproline.…”

Section: Pyridinoline and Hydroxyproline Analysismentioning

confidence: 99%

Covalent cross-linking of liver collagen by pyridinoline increases in the course of experimental alveolar echinococcosis

et al. 1995

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Summary :We report that coval ent cross-linking of collagen mol ecul es by pyridinoline increases significantly in liver in a muri ne model of alveolar echinococcosis. The highest amount of pyridinoline per collagen mol ecul e (up to 3.5 fold the control values) is found in liver parasitic lesions. It is also increased, but to a far lesser extent, at distance from the fibrotic areas, in macroscopically normal zones of the liver, suggesting that the increase in mature collagen cross-linking occurring in the fibrogenesis due to Echinococcus multilocularis infection involves the whole liver. The comparison of these data with those we have obtai ned in another parasitic disease, muri ne schistosomiasis leading to a milder liver fibrosis, largely reversible following chemotherapy, supports a relationship between the liver pyridinoline level and the severity of liver fibrosis. Pyridinoline coul d be a tissular marker of chronic liver fibrosis in parasitic diseases.

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The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid

Cited by 3,604 publications

References 19 publications

Engineering Musculoskeletal Tissues with Human Embryonic Germ Cell Derivatives

Engineering Musculoskeletal Tissues with Human Embryonic Germ Cell Derivatives

Selective and non-selective metalloproteinase inhibitors reduce IL-1-induced cartilage degradation and loss of mechanical properties

Covalent cross-linking of liver collagen by pyridinoline increases in the course of experimental alveolar echinococcosis

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