The Determination of Glucose by the Ortho-Toluidine Method (Filtrate and Direct Procedure)

Cooper, Gerald R.; Mcdaniel, Valeta; Baer, Daniel M.; Dubowski, Kurt M.; Morrison, Daphne; Vanderlinde, Raymond E.; Fasce, Charles F.; Kowalski, P.

doi:10.1016/b978-0-12-609106-9.50021-x

Cited by 81 publications

(24 citation statements)

References 14 publications

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“…Plasma glucose was estimated by O-Toluidine method [52]. 5 mL of O-toluidine colour reagent was taken in a test tube and 0.1 mL of plasma was added.…”

Section: Biochemical Assaymentioning

confidence: 99%

Acute and sublethal effects in an Indian major carp Cirrhinus mrigala exposed to silver nitrate: Gill Na+/K+-ATPase, plasma electrolytes and biochemical alterations

Sathya

Ramesh

Poopal

et al. 2012

Fish & Shellfish Immunology

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“…Plasma glucose was estimated by O-Toluidine method [52]. 5 mL of O-toluidine colour reagent was taken in a test tube and 0.1 mL of plasma was added.…”

Section: Biochemical Assaymentioning

confidence: 99%

Acute and sublethal effects in an Indian major carp Cirrhinus mrigala exposed to silver nitrate: Gill Na+/K+-ATPase, plasma electrolytes and biochemical alterations

Sathya

Ramesh

Poopal

et al. 2012

Fish & Shellfish Immunology

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“…Plasma glucose levels were estimated using the o-toluidine method [29], while total plasma protein levels were determined following the methods of Lowry et al [30]. Plasma AST and ALT levels were determined following the methods of Reitman and Frankel [31].…”

Section: Biochemical Estimationmentioning

confidence: 99%

Physiological effects of paraquat in juvenile African catfish Clarias gariepinus (Burchel 1822)

2014

JCLM

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“…Glucose was estimated by two methods: (1) o-toluidine method (Cooper and McDaniel, 1970); (2) Boehringer GOD/period method for blood glucose. Lactate was estimated by a Boehringer enzymic kit and adenosine triphosphate (ATP) was measured by the method of Stanley and Williams (1969).…”

Section: Biochemical Estimationsmentioning

confidence: 99%

METABOLISM OF BABESIA PARASITES IN VITRO. GLUCOSE AND ENERGY METABOLISM AND SURVIVAL OF BABESIA RODHAINI IN A BASAL MEDIUM WITH AND WITHOUT ADENOSINE

1982

Aust J Exp Biol Med

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Summary.During in vitro incubation in Krebs Ringer phosphate (KRP) medium, rat erythrocytes about 20% parasitized with B. rodhaini used two to four times as much gtucose as unparasitized erythrocytes. However, the ATP concentration and ATP production of parasitized erythrocytes decreased. For example, before incubation the ATP concentration in parasitized erythrocytes was significantly higher than unparasitized erythrocytes, but following 0-5 h incubation it was reduced to about the same as in unparasitized erythrocytes.Addition of 5 X 10-3 M adenosine to the basal medium increased the ATP production and the adenylate energy charge of parasitized erythrocytes. Infectivity tests in susceptible mice showed that, despite these seemingly favourable changes in energy metabolism, adenosine had no detectable effect on the survival of B. rodhaini. Absence of infectivity showed the inability of KRP medium to support B. rodhaini for 22 h in vitro with or without adenosine.Adenosine also greatly decreased the glucose uptake by parasitized erythrocytes, apparently by competitive inhibition, since lactate production was unchanged. INTRODUCTIONStudies of the metabolism of haemoprotozoan parasites of the genus Babesia are relevant to the production of living vaccines against bovine infections (Callow, 1977). The present series of investigations was designed to study the in vitro metabolism and survival in basal salts media of Babesia rodhaini.Metabolic studies of Babesia rodhaini (Rickard, 1969;Momen, 1979) provided information on the in vitro metabolism, particularly carbohydrate metabolism, of the parasite in basal salts media. There was no information, however, on the in vitro survival or energy metabolism of the parasite in basal media.

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The Determination of Glucose by the Ortho-Toluidine Method (Filtrate and Direct Procedure)

Cited by 81 publications

References 14 publications

Acute and sublethal effects in an Indian major carp Cirrhinus mrigala exposed to silver nitrate: Gill Na+/K+-ATPase, plasma electrolytes and biochemical alterations

Acute and sublethal effects in an Indian major carp Cirrhinus mrigala exposed to silver nitrate: Gill Na+/K+-ATPase, plasma electrolytes and biochemical alterations

Physiological effects of paraquat in juvenile African catfish Clarias gariepinus (Burchel 1822)

METABOLISM OF BABESIA PARASITES IN VITRO. GLUCOSE AND ENERGY METABOLISM AND SURVIVAL OF BABESIA RODHAINI IN A BASAL MEDIUM WITH AND WITHOUT ADENOSINE

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