The Determination of Estrone in Oil Solutions of Estrogens*

Colorimetric Determination of alpha-Estradiol in Estrogenic Mixtures Using Partition Chromatography†

“…The following items and directions are additions to or modifications of the procedures described in preceding papers (1,2).…”

“…separatory funnel with 25 ml. of Skellysolve C. Proceed by the method of Carol and Rotondaro (2) to the point where the solution obtained in the Girard reaction has been neutralized. Extract at once with three successive 15-1111. portions of chloroform.…”

“…Many commercial estrogenic preparations are complex mixtures of ketosteroids and their reduction products, the diols. Methods have been developed in this laboratory for the gravimetric determination of the total ketosteroids (2) and for their individual determination by colorimetry (3) and infrared spectrophotometry (4). One of the authors ( 5 ) has also published a method for the infrared spectrophotometric assay of the estrogenic diols, incorporating for some mixtures the partition chromatographic system described herein for the separation of the dihydroequilenins from a-dihydroequilin and the estradiols.…”

Determination of Estrogens in Dosage Forms by Fluorescence Using Dansyl Chloride

“…IR quantitative analysis has been used to measure estrone, equilin, and equilenin after formation of the benzenesulfonate phenolic esters (8). The reaction with Girard's reagent T is the basis of another IR spectrophotometric procedure that is somewhat nonspecific (9). Polarographic analysis of the Girard T (10) and nitrosophenol (11) derivatives of estrogens has been reported.…”

The colorimetric Determination of Estrone, Equilin and Equilenin in Mixtures

“…The alcoholic derivatives of the ketosteroids, the estrogenic diols, behaved in a manner analogous to that of the parent substances. These phenolic alcohols were separated from the ketones by the use of Girard Reagent T, according to the procedure of Carol and Rotondaro (8). Other phenols, acidic substances, and sterols in gravid mares' urine were also removed following that Absorption spectrum, 300 micrograms equilin/7 rnl.…”