IN continuing the investigation of the relation of blood-calcium to the formation of cataract in rabbits, we found that there are certain conditions under which the serum-calcium shows considerable variations. It is true that one of us [Adams, 1930] previously obtained satisfactory control experiments in which only very slight variations were observed; but, with repeated experiments, it became apparent that the level of serum-calcium may vary considerably in individual rabbits, over long periods, and in particular with different diets.The evidence of other observers is conflicting. Thus Davies, Dickens and Dodds [1926] state that in individual rabbits the serum-calcium does not vary more than 1*5 mg. per 100 cc. serum. Ssewerin [1927] states that the serumcalcium level remains unchanged when cabbage is fed to a starving rabbit. He gives no figures. On the other hand, Ipponsugi [1927, 1], in fifteen control rabbits, found the serum-calcium varying from 13-5 to 18 mg. per 100 cc. and in ten other controls he found variations from 12 to 17 mg. per 100 cc. Harnes [1928] determined the serum-calcium in eighty rabbits throughout one year and found variations from 14-5 to 18-5 mg. per 100 cc. Culhane [1927] reports variations, in six rabbits fed on oats and cabbage, from 14-31 to 17-12 mg. Ca per 100 cc. serum. Kapsinow and Underhill [1929] observed considerable daily variations in the serum-calcium level, usually between 10 and 15 mg. per 100 cc. serum, with one figure as high as 21 mg. per 100 cc.In addition there exists a further uncertainty as to the effect of diet' on the serum-calcium in this animal. Culhane [1927Culhane [ , 1930 reported that cabbage causes a rise in the serum-calcium, but Kapsinow and Underhill [1929] were unable to confirm this. Cannon and Greenwood [1930] found that a diet of sweet clover caused the serum-calcium of young rabbits to decrease.1 After this paper had been sent to press, we received the copy of the Journal of Biological Chemistry for December, 1931, containing an article by Dupr6 and Semeonoff [1931] in which the effects of diet on serum-calcium and phosphorus in the rabbit are described. The results obtained by these authors are in agreement with those which we report in this paper.