The determination of cadmium and mercury in blood of smokers and non-smokers

Extraction optimization using Box–Behnken design and method validation for ethanol in oral fluid

“…The method proposed by the HS technique is simpler and less expensive in comparison to techniques such as solid phase microextraction, as performed by Feltraco et 27 Considering the time of HS extraction and the chromatographic run, the total time required for the analysis of one sample is 17 minutes. This shows the quickness of the HS-GC/FID and HS-GC/MS methods, and therefore, the advantage of its implementation in the laboratory routine.…”

“…This nding is opposite to that presented so far in the literature for ethanol analysis, where a stirring time of up to 10 min is usually used. [25][26][27] The predictive capacity of the mathematical model generated was assessed by analyzing ve replicates of the same sample under optimal extraction conditions: heating temperature 90 C; stirring time 7 min; injection volume 1000 mL. The predictive capacity of the model was attested by the high similarity between the predicted area and experimental area obtained (Table 2).…”

“…This demonstrates the increased sensitivity of the GC/MS technique compared to our GC/FID method and the methods already described in the literature. [25][26][27][28] 3.2.4 Recovery. Recovery was assessed by comparing the results obtained in water and OF using a calibration curve prepared on the day of analysis for the LQD, MCQ, and HQC samples to obtain suitable values for the proposed method.…”

“…However, in other tests, where routine storage and analysis was reproduced, the samples showed no signicant variation, allowing collection, preparation, and transportation under the usual conditions required for its use to monitor drivers under the inuence of ethanol. These results are of great importance, since the methods described in the literature typically show no data, or have insufficient data, regarding the stability [25][26][27][28] despite being recommended by the validation guidelines for bioanalytical methods. 22,23 3.2.7 Selectivity.…”