The Determination of Anabolic Steroids by MECC, Gradient HPLC, and Capillary GC

Lurie, Ira S.; Sperling, Albert R.; Meyers, Richard P.

doi:10.1520/jfs13572j

Cited by 28 publications

(12 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on these grounds, CE is an interesting new method that is highly complementary to the traditional analytical approaches. This latter aspect was shown with MECC data of anabolic steroids of forensic interest that were compared with data obtained by HPLC and GC [75]. The advantages over GC are the ability to deal with nonvolatile and thermally labile compounds whereas those over HPLC are higher efficiency and resolving power, and much reduced consumption of expensive and often toxic organic solvents.…”

Section: General Aspects and Areas Of Applicationmentioning

confidence: 86%

“…screening these urines for misused and banned drugs. Examples studied for potential use in doping control include anabolic steroids [75], diuretics [93], ephedrine and norephedrine [94], diuretics, narcotics-analgesics and P-blockers [95]. Detection of such drugs and/ or their metabolites in routine urinary screening of athletes leads to disqualification and the implementation of disciplinary action.…”

Section: Forensic Drug Analysismentioning

confidence: 99%

See 1 more Smart Citation

Capillary electrophoresis in clinical and forensic analysis

Heeren

Thormann

1997

Electrophoresis

View full text Add to dashboard Cite

During the past decade, capillary electrophoresis (CE) emerged as a promising, effective and economic approach for separation of a large variety of substances, including those encountered in clinical and forensic analysis. Reliable and automated CE instruments became commercially available and promoted the exploration of an increasing number of CE methods and fields of application. The widespread applicability of CE, its enormous separation power and high-sensitivity detection schemes make this technology an attractive and promising tool. This review discusses the principles and important aspects of CE-based assays and provides an overview of the key achievements encountered with CE in clinical and forensic analysis, including those associated with the analysis of serum proteins, hemoglobin variants, drugs and nucleic acids. Validated assays, interesting applications and future trends in clinical and forensic analysis are also discussed.

show abstract

Section: General Aspects and Areas Of Applicationmentioning

confidence: 86%

Section: Forensic Drug Analysismentioning

confidence: 99%

Capillary electrophoresis in clinical and forensic analysis

Heeren

Thormann

1997

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…In other application fields, e.g., toxicology, HPLC and CE have shown different selectivity providing "orthogonal" separations [13,14]. Thus, their use in combination greatly enhances the identification power and the analytical reliability.…”

Section: Introductionmentioning

confidence: 99%

Analysis of organic components of smokeless gunpowders: High‐performance liquid chromatogaphy vs. micellar electrokinetic capillary chromatography

et al. 2004

View full text Add to dashboard Cite

The aim of the present study was to verify the analytical performances of high-performance liquid chromatography (HPLC) and micellar electrokinetic capillary chromatography (MEKC) for the separation and qualitative determination of a selected group of organic components of smokeless gunpowders. The HPLC method was based on a gradient reversed-phase elution with a mobile phase composed of 0.17 M H(3)PO(4)/methanol; detection was performed by UV absorption at the wavelengths of 220, 254, and 270 nm. The MEKC experiments were carried out by using uncoated fused-silica capillaries (50 microm inside diameter, 50 cm effective length) and a running buffer composed of 10 mM sodium tetraborate at pH 9.24 added with 25 mM sodium dodecyl sulfate (SDS); the applied voltage was 25 kV; detection was either at a fixed wavelength UV of 214 nm or with a diode-array detector operating in the wavelength range from 190 to 350 nm. Both reversed-phase HPLC and MEKC techniques succeeded in resolving the tested standard mixtures of organic components of smokeless powders. Although the sequence of elution of the different analytes was slightly different between HPLC and MEKC, a statistical analysis based on the Spearman's rank correlation test showed that the two separation patterns were highly correlated. HPLC and MEKC were comparable in terms of elution/migration time precision, whereas MEKC showed higher reproducibility of peak areas. The interfacing of capillary electrophoresis with diode array UV detection provided distinct UV spectra of the individual analytes, thus improving, on the detection side, the analytical selectivity and identification power of capillary electrophoresis.

show abstract

“…Previously, Vindevogel and Sandra used a PlackettBurman statistical design to optimize the separation of four testosterone esters [8]. Lurie et al monitored the separation of 24 anabolic steroids at 3 different wavelengths [9]. In these two studies, the separations were performed in the presence of a high acetonitrile content.…”

Section: Introductionmentioning

confidence: 99%

Separation of anabolic steroids by micellar electrokinetic capillary chromatography

1999

View full text Add to dashboard Cite

SummaryThe separation of seven analogous anabolic steroids was studied by micellar electrokinetic capillary chromatography (MECC). The retention order was found to be dependent on polarity. All of these steroids were well separated by the addition of organic modifiers to the separation buffer. Of the organic modifiers tested, 1-propanol gave the best separation, better than methanol or acetonitrile.

show abstract

The Determination of Anabolic Steroids by MECC, Gradient HPLC, and Capillary GC

Cited by 28 publications

References 8 publications

Capillary electrophoresis in clinical and forensic analysis

Capillary electrophoresis in clinical and forensic analysis

Analysis of organic components of smokeless gunpowders: High‐performance liquid chromatogaphy vs. micellar electrokinetic capillary chromatography

Separation of anabolic steroids by micellar electrokinetic capillary chromatography

Contact Info

Product

Resources

About