The Determinant of Potyvirus Ability to Overcome the RTM Resistance of <i>Arabidopsis thaliana</i> Maps to the N-Terminal Region of the Coat Protein

Decroocq, Véronique; Salvador, Beatriz; Sicard, Ophélie; Glasa, Miroslav; Cosson, Patrick; Svanella-Dumas, Laurence; Revers, Frédéric; Garcı́a, Juan Antonio; Candresse, Thierry

doi:10.1094/mpmi-22-10-1302

Cited by 98 publications

(70 citation statements)

References 52 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this case, the RTM factors could sequester this long-distance movement-capable form of the virus, thereby blocking viral movement through the vascular tissue. In the case of potyvirus isolates able to overcome the RTM resistance (Decroocq et al, 2009), mutated positions in the N-terminal end of the CP may not allow the postulated interaction between the RTM complex and the CP to occur, leading to an absence of sequestration and long-distance movement of virus. However, in our study, no interaction has been detected so far between the RTM proteins and the CPs of LMV and PPV, but these negative results do not rule out the possibility that such interactions could occur in plant cells, since a number of artifactual effects may also be responsible for them.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, as mutations in the potyvirus CP are sufficient to overcome the RTM resistance (Decroocq et al, 2009), it has been suggested that the CP may interact with the RTM proteins. The three RTM cDNAs and CPs from two virus isolates unable to overcome RTM resistance (LMV-AF199 and PPV-PS) and from virus isolates able to overcome RTM resistance (LMV-AFVAR1 [Decroocq et al, 2009] and PPV-R) were cloned in the yeast two-hybrid vectors. The potential interactions between the RTM proteins and between the CPs and the RTM proteins were then evaluated.…”

Section: Rtm3 Interacts With Itself and With Rtm1mentioning

confidence: 99%

“…RTM2 encodes a protein that contains a transmembrane domain and has similarities to small heat shock proteins, although its expression is not heat inducible and does not contribute to thermotolerance . Although it is not understood how the RTM proteins restrict long-distance movement of potyviruses, it has been shown that both RTM1 and RTM2 are expressed in phloem-associated tissues, that the corresponding proteins localize to phloem sieve elements (Chisholm et al, 2001), and that the N-terminal end of the potyviral capsid protein (CP) is involved in breaking of the RTM resistance (Decroocq et al, 2009), suggesting a direct or indirect interaction between the potyvirus CP and the RTM factors.…”

mentioning

confidence: 99%

See 2 more Smart Citations

RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein

Cosson

Sofer

et al. 2010

Plant Physiology

View full text Add to dashboard Cite

Restriction of long-distance movement of several potyviruses in Arabidopsis (Arabidopsis thaliana) is controlled by at least three dominant restricted TEV movement (RTM) genes, named RTM1, RTM2, and RTM3. RTM1 encodes a protein belonging to the jacalin family, and RTM2 encodes a protein that has similarities to small heat shock proteins. In this article, we describe the positional cloning of RTM3, which encodes a protein belonging to an undescribed protein family of 29 members that has a meprin and TRAF homology (MATH) domain in its amino-terminal region and a coiled-coil domain at its carboxy-terminal end. Involvement in the RTM resistance system is the first biological function experimentally identified for a member of this new gene family in plants. Our analyses showed that the coiled-coil domain is not only highly conserved between RTM3-homologous MATH-containing proteins but also in proteins lacking a MATH domain. The cluster organization of the RTM3 homologs in the Arabidopsis genome suggests the role of duplication events in shaping the evolutionary history of this gene family, including the possibility of deletion or duplication of one or the other domain. Protein-protein interaction experiments revealed RTM3 self-interaction as well as an RTM1-RTM3 interaction. However, no interaction has been detected involving RTM2 or the potyviral coat protein previously shown to be the determinant necessary to overcome the RTM resistance. Taken together, these observations strongly suggest the RTM proteins might form a multiprotein complex in the resistance mechanism to block the long-distance movement of potyviruses.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Rtm3 Interacts With Itself and With Rtm1mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein

Cosson

Sofer

et al. 2010

Plant Physiology

View full text Add to dashboard Cite

show abstract

“…Notably, both RTM1 and RTM2 are expressed in phloem-associated tissues and sieve elements, correlating with their function in impeding virus movement. Interestingly, the N terminus of the potyvirus coat protein contains features that can overcome RTM resistance, suggesting that interactions of RTM proteins with viral CPs may mediate resistance responses (Decroocq et al, 2009). Further studies on LMR should reveal the mechanism of this intriguing antiviral resistance response.…”

Section: R Gene-mediated Responses To Virus Infectionmentioning

confidence: 99%

Plant Immune Responses Against Viruses: How Does a Virus Cause Disease?

2013

View full text Add to dashboard Cite

Plants respond to pathogens using elaborate networks of genetic interactions. Recently, significant progress has been made in understanding RNA silencing and how viruses counter this apparently ubiquitous antiviral defense. In addition, plants also induce hypersensitive and systemic acquired resistance responses, which together limit the virus to infected cells and impart resistance to the noninfected tissues. Molecular processes such as the ubiquitin proteasome system and DNA methylation are also critical to antiviral defenses. Here, we provide a summary and update of advances in plant antiviral immune responses, beyond RNA silencing mechanisms-advances that went relatively unnoticed in the realm of RNA silencing and nonviral immune responses. We also document the rise of Brachypodium and Setaria species as model grasses to study antiviral responses in Poaceae, aspects that have been relatively understudied, despite grasses being the primary source of our calories, as well as animal feed, forage, recreation, and biofuel needs in the 21st century. Finally, we outline critical gaps, future prospects, and considerations central to studying plant antiviral immunity. To promote an integrated model of plant immunity, we discuss analogous viral and nonviral immune concepts and propose working definitions of viral effectors, effector-triggered immunity, and viral pathogen-triggered immunity.

show abstract

“…Genetic characterization from natural ecotype variations and chemically induced mutants has revealed that at least three dominant genes named RTM1, RTM2 and RTM3 are involved in the restriction of long-distance movement of potyviruses in the Arabidopsis accession Columbia (Col-0) (Mahajan et al, 1998;Whitham et al, 1999). These RTM factors affect the potyvirus long-distance transport through direct or indirect interaction with CP (Decroocq et al, 2009). …”

Section: Smv Cell-to-cell and Long-distance Movementmentioning

confidence: 99%