The detection of spermine and spermidine in human in vitro fertilization supernatants and their relation to early embryo-associated suppressor activity

Lea, Richard G.; Porat, Offie; Daya, Salim; Fujiwara, Katsuo; Clark, David A.

doi:10.1016/s0015-0282(16)54614-7

Cited by 9 publications

(2 citation statements)

References 6 publications

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“…[ 6 ] Consistent with this hypothesis, animal experiments have shown that SPD can improve the success rate of embryo implantation and development. [ 7 ] More intriguingly, several recent works have demonstrated a broader immunomodulatory function for SPD, such as anti‐inflammatory effects [ 8 ] and improvement in anti‐tumor immunity. [ 9 ] Hence, we have envisioned that the local administration of SPD in biomaterials can reduce the inflammation induced by exogenous biomaterials and promote wound healing, thus avoiding the undesirable adverse reactions caused by immunosuppressive drugs.…”

Section: Introductionmentioning

confidence: 99%

Spermidine‐Functionalized Injectable Hydrogel Reduces Inflammation and Enhances Healing of Acute and Diabetic Wounds In Situ

Wu,

Yang,

Fan

et al. 2024

Advanced Science

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The inflammatory response is a key factor affecting tissue regeneration. Inspired by the immunomodulatory role of spermidine, an injectable double network hydrogel functionalized with spermidine (DN‐SPD) is developed, where the first and second networks are formed by dynamic imine bonds and non‐dynamic photo‐crosslinked bonds respectively. The single network hydrogel before photo‐crosslinking exhibits excellent injectability and thus can be printed and photo‐crosslinked in situ to form double network hydrogels. DN‐SPD hydrogel has demonstrated desirable mechanical properties and tissue adhesion. More importantly, an “operando” comparison of hydrogels loaded with spermidine or diethylenetriamine (DETA), a sham molecule resembling spermidine, has shown similar physical properties, but quite different biological functions. Specifically, the outcomes of 3 sets of in vivo animal experiments demonstrate that DN‐SPD hydrogel can not only reduce inflammation caused by implanted exogenous biomaterials and reactive oxygen species but also promote the polarization of macrophages toward regenerative M2 phenotype, in comparison with DN‐DETA hydrogel. Moreover, the immunoregulation by spermidine can also translate into faster and more natural healing of both acute wounds and diabetic wounds. Hence, the local administration of spermidine affords a simple but elegant approach to attenuate foreign body reactions induced by exogenous biomaterials to treat chronic refractory wounds.

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Section: Introductionmentioning

confidence: 99%

Spermidine‐Functionalized Injectable Hydrogel Reduces Inflammation and Enhances Healing of Acute and Diabetic Wounds In Situ

Wu,

Yang,

Fan

et al. 2024

Advanced Science

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“…Until now, the typical methods for spermine analysis have mainly focused on immunoassays, 4 or chromatographic techniques. 5 Even though it is not necessary to derivatize, the complex procedure and long biorecognition time reduce the efficiency of the immunoassay. For chromatographic techniques, it requires the active optical properties of analytes.…”

Section: Introductionmentioning

confidence: 99%

Calf thymus DNA-stabilized polythiophene fluorescence probe for label-free detection of spermine

Wang

Zhang

Liu

et al. 2012

Analyst

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It is a challenge to detect molecules lacking a chromophore, such as polyamines, by optical methods since they are insensitive to light. In order to detect the optical signals, it is compulsory to derive these molecules with optical labels, which, however, is complicated, time-consuming and may be expensive. In this work, a highly specific strategy for spermine detection is developed on the basis of the iodide-induced conformational change of polythiophene. By using the triplex complex of negatively charged double-stranded calf thymus DNA (ctDNA)-stabilized cationic polythiophene as a fluorescent probe, the highly specific detection of spermine could be realized since polythiophene, which will be released from the triplex complex owing to the condensation and aggregation of ctDNA with spermine, undergoes a conformational change from the random-coiled non-planar state to the highly conjugated planar form in the presence of iodide, resulting in a yellow-to-red color conversion and fluorescence quenching. The quenched fluorescence was found to be proportional to the spermine concentrations in the range of 1.2-50 μM with the limit of detection (LOD) being 0.5 μM (3σ/k).

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A free-radical hypothesis for the instability and evolution of genotype and phenotypein vitro

Parchment

Natarajan

1992

Cytotechnology

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It has been known for several decades that cultured murine cells undergo a defined series of changes, i.e., an in vitro evolution, which includes crisis, spontaneous transformation ('immortalization'), aneuploidy, and spontaneous neoplastic transformation. These changes have been shown to be caused by the in vitro environment rather than an inherent instability of the murine phenotype or genotype. Serum amine oxidases were recently identified as a predominant cause of crisis. These enzymes generate hydrogen peroxide from polyamine substrates that enter the extracellular milieu. This finding implicates free-radical toxicity as the underlying cause of in vitro evolution. We propose an oxyradical hypothesis to explain each of the stages of in vitro evolution and discuss its significance for cytotechnology and long-term cultivation of mammalian cell types.

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The detection of spermine and spermidine in human in vitro fertilization supernatants and their relation to early embryo-associated suppressor activity

Cited by 9 publications

References 6 publications

Spermidine‐Functionalized Injectable Hydrogel Reduces Inflammation and Enhances Healing of Acute and Diabetic Wounds In Situ

Spermidine‐Functionalized Injectable Hydrogel Reduces Inflammation and Enhances Healing of Acute and Diabetic Wounds In Situ

Calf thymus DNA-stabilized polythiophene fluorescence probe for label-free detection of spermine

A free-radical hypothesis for the instability and evolution of genotype and phenotypein vitro

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