The Detection of Mutations in Drosophila melanogaster

Abrahamson, S.; Lewis, E. B.

doi:10.1007/978-1-4615-8969-3_7

Cited by 56 publications

(13 citation statements)

References 35 publications

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“…These offer promise for monitoring the environment to decrease harmful exposure in human populations. The-work of Ames in calling attention to the need for such monitoring systems and in providing one that is simple and effective is especially noteworthy (5) although various other tests have also been proposed (6)(7)(8)(9)(10)(11)(12).…”

mentioning

confidence: 99%

Measurement of mutagenesis in mammalian cells.

Waldren¹,

Jones²,

Puck³

1979

Proc. Natl. Acad. Sci. U.S.A.

118

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A method using mammalian cells in vitro for detection and quantitation of mutagenic actions that appears to be useful for screening for carcinogenesis and genetic damage by environmental agents is presented. The method involves use of stable human--Chinese hamster ovary hybrid cells that have retained a single human chromosome not necessary for cell reproduction. Forward mutations are detected in genes necessary for production of specific human cell surface antigens. Such mutants form colonies in the presence of specific antisera and complement that destroy the unmutagenized cells. Use of the method is illustrated for the action of x-irradiation, N-methyl-N'-nitro-N-nitrosoguanidine, and caffeine. The method appears to be unique in that it permits assessment of lesions that cause loss of all or most of the chromosome as well as various localized gene mutations. The former action is particularly important because of the major involvement of chromosomal lesions in an extremely important class of human genetic disease.

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mentioning

confidence: 99%

Measurement of mutagenesis in mammalian cells.

Waldren¹,

Jones²,

Puck³

1979

Proc. Natl. Acad. Sci. U.S.A.

118

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“…Spontaneous sex-linked recessive lethal mutations. The frequencies of spontaneous sex-linked recessive lethal mutations were determined by standard Drosophila techniques (Abrahamson & Lewis, 1971) using the X-chromosome balancer FM7 (Merriam, 1968), which contained the markers y 31d w a v B. Mutation frequencies were determined in both males and females in a stock which contained the mutator gene, v f 3N Mit-f 3^ car, and in a stock that did not contain the mutator gene, v f 3N Mu-P N+ B. The mutation frequency in males was determined by crossing males which contained or did not contain the mutator gene to virgin FM7/FM7 females in mass.…”

Section: Methodsmentioning

confidence: 99%

The control of mutational instability by a new mutator gene ofDrosophila melanogaster

Woodruff¹

1975

Genet. Res.

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The isolation and genetic characterization of a new mutator gene, Mutator-forked , of Drosophila melanogaster are described. This mutator gene is unique in that it seems to increase specifically the reversion frequency of the unstable mutant forked 3N (J 3N , 1-56.7), since the frequency of spontaneous sex-linked recessive lethals in males and females and the frequency of reverse mutations at eight additional X-linked alleles were unaffected by Mu-f 3N . The mutator is a dominant gene that has been mapped to the region between f 3N (1-56.7) and Beadex-2 (Bx 2 , 1-59.4) in the X chromosome, and it seems to function only in the ' cis' configuration. The mode of action of Mu-p N is compared with that of other mutator genes.

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“…Acute toxicity to fish is required in the (21). Various experiments have shown that this assay can be used as a teratogen screen; in mechanistic studies of abnormal development, it can be used to investigate gene involvement in teratogenic resistance, and the possible role of heat-shock proteins in preventing birth defects (21)(22)(23)(24).…”

Section: Acute Effectsmentioning

confidence: 99%

13th Meeting of the Scientific Group on Methodologies for the Safety Evaluation of Chemicals (SGOMSEC): alternative testing methodologies for ecotoxicity.

Walker¹,

Kaiser²,

Klein³

et al. 1998

Environ Health Perspect

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There is growing public pressure to minimize the use of vertebrates in ecotoxicity testing; therefore, effective alternatives to toxicity tests causing suffering are being sought. This report discusses alternatives and differs in some respects from the reports of the other three groups because the primary concern is with harmful effects of chemicals at the level of population and above rather than with harmful effects upon individuals.

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The Detection of Mutations in Drosophila melanogaster

Cited by 56 publications

References 35 publications

Measurement of mutagenesis in mammalian cells.

Measurement of mutagenesis in mammalian cells.

The control of mutational instability by a new mutator gene ofDrosophila melanogaster

13th Meeting of the Scientific Group on Methodologies for the Safety Evaluation of Chemicals (SGOMSEC): alternative testing methodologies for ecotoxicity.

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