The detection of monkeypox virus DNA in wastewater samples in the Netherlands

Jonge, Eline F. de; Peterse, Céline; Koelewijn, Jaap; Drift, Anne-Merel R. van der; Beek, Rudolf F.H.J. van der; Nagelkerke, Erwin; Lodder, Willemijn J.

doi:10.1016/j.scitotenv.2022.158265

Cited by 71 publications

(34 citation statements)

References 8 publications

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“…Interestingly, we consistently detected MPXV DNA in samples collected in WWTP18 (Barcelona) since week 22_23, when only 39 cumulative cases were declared in the entire Autonomous Community of Catalonia. In line, MPXV DNA was also detected in week 22_21 on wastewater samples collected from Schiphol Airport and in different Dutch WWTPs from week 22_22 onwards (de Jonge et al, 2022).…”

Section: Discussionmentioning

confidence: 69%

“…According with this model, wastewater-based epidemiology (WBE) can detect on average 7 MPX cases out of 100,000 people. Currently, various studies detected MPXV DNA in wastewater worldwide (de Jonge et al, 2022; la Rosa et al, 2022; Wolfe et al, 2022), highlighting again wastewater analysis as a non-invasive tool for monitoring the status and trend an emerging infection. The aim of the present study was to trace the community circulation of the MPXV from potentially symptomatic, asymptomatic, or presymptomatic individuals using the previous established Spanish National SARS-CoV-2 Wastewater Surveillance Network (VATar COVID-19).…”

Section: Introductionmentioning

confidence: 99%

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Wastewater based epidemiology beyond SARS-CoV-2: Spanish wastewater reveals the current spread of Monkeypox virus

Guzman

Díaz-Reolid

Truchado

et al. 2022

Preprint

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Besides nasopharyngeal swabs, monkeypox virus (MPXV) DNA has been detected in a variety of samples such as saliva, semen, urine and fecal samples. Using the environmental surveillance network previously developed in Spain for the routine wastewater surveillance of SARS-CoV-2 (VATar COVID-19), we have analyzed the presence of MPXV DNA in wastewater from different areas of Spain. Samples (n=312) from 24 different wastewater treatment plants were obtained between May 9 (week 22_19) and August 4 (week 22_31), 2022. Following concentration of viral particles by flocculation, a qPCR procedure allowed us to detect MPXV DNA in 63 wastewater samples collected from May 16 to August 4, 2022, with values ranging between 2.2 per 103 to 8.7 per 104 genome copies (gc)/L. This study shows that MPXV DNA can be reproducibly detected by qPCR in longitudinal samples collected from different Spanish wastewater treatment plants. According to data from the National Epidemiological Surveillance Network (RENAVE) in Spain a total of 6,119 cases have been confirmed as of August 19, 2022. However, and based on the wastewater data, the reported clinical cases seem to be underestimated and asymptomatic infections may be more frequent than expected.

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Section: Discussionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Wastewater based epidemiology beyond SARS-CoV-2: Spanish wastewater reveals the current spread of Monkeypox virus

Guzman

Díaz-Reolid

Truchado

et al. 2022

Preprint

View full text Add to dashboard Cite

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“…Other studies reported detection of MPXV genome in wastewater in the Netherlands, USA, and Italy . This reported the detection and quantification of MPXV genomes by digital PCR in sewersheds in France.…”

Section: Resultsmentioning

confidence: 99%

“…However, the risk of contamination of this peridomestic fauna have to be investigated in correlation with the study of persistence of MPXV in wastewater. 30,31 Other studies reported detection of MPXV genome in wastewater in the Netherlands, 32 USA, 33 and Italy. 34 This reported the detection and quantification of MPXV genomes by digital PCR in sewersheds in France.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

First Detection of Monkeypox Virus Genome in Sewersheds in France: The Potential of Wastewater-Based Epidemiology for Monitoring Emerging Disease

Wurtzer

Levert

Dhenain

et al. 2022

Environ. Sci. Technol. Lett.

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A monkeypox virus outbreak has been spreading in multiple nonendemic countries since May 2022. The atypical clinical profile of patients has led to a very likely underestimation of the number of cases at the beginning of the epidemic. The detection and quantification of the Monkeypox virus genome in sewersheds in Paris (France) correlated temporally with the identification of the first case of infection and the spread of the disease within the population connected to the sewage system.

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“…For example, Hemati et al proposed useful study of the using of procedures which were taken of the spreading of the corona virus disease 2019 [27]. de Jonge et al found in Netherland that there is deoxyribonucleic acid (DNA) of the monkey pox virus in the wastewater samples [28]. Rao et al study the detection of the monkey pox virus disease of traveller from Africa [29].…”

Section: Introductionmentioning

confidence: 99%

The basic reproductive ratio of the 2022 outbreak of the monkey pox virus disease for the United Kingdom, Canada, Brazil, the United Arab Emirates, and Nigeria

Al‐Raeei

2022

Beni-Suef Univ J Basic Appl Sci

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Background A recent outbreak of the monkey pox virus disease (MPVD) started to spread over the world before the second half of the 2022 year. This outbreak of the monkey pox virus disease is known as the 2022 outbreak of the monkey pox virus disease. The monkey pox virus disease is a type of the pox disease similar to the human one. This disease is an endemic in some African countries; however, a new spreading of this disease started to appear in other countries, such as the Spain, brazil, Greece, the United Kingdom, and Portugal, Australia, and the USA. As of the end of September 2022, the MPVD spread over than 107 countries over the world. Results This study focuses on the employing of the simplest model of the diseases forecasting which is SIRD model for the finding of the basic reproductive ratio of the monkey pox virus disease in multiple countries over the world where the disease spreads. The model takes into accounts the number of the susceptible people, the number of the infectious people, the number of the recovered people, and the number of the deceased people. Based on the results of the SIRD model coefficients, we find that the basic reproductive ratio values of the recent spreading of the monkey pox virus disease are 1.3274 for the United Kingdom where the first case of the disease was recorded, 1.0714 for the United Arab Emirates, 1.0866 for Nigeria, 1.5589 for Brazil, and 1.3610 for Canada. Conclusions We find that the average value of the basic reproductive ratio of the 2022 outbreak of the monkey pox virus disease is about 1.2809. This important result of our calculations predicts that the 2022 outbreak of the monkey pox virus disease is turned into pandemic over the world. The things which confirm this result, based on our calculations, are the values of the basic reproductive ratio of the 2022 outbreak of the disease in the considered countries from multiple continents where all the values of the basic reproductive ratio are bigger than one. From this point, the counties over the world must apply multiple procedures for limiting the spreading of the monkey pox virus disease. Graphical Abstract

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The detection of monkeypox virus DNA in wastewater samples in the Netherlands

Cited by 71 publications

References 8 publications

Wastewater based epidemiology beyond SARS-CoV-2: Spanish wastewater reveals the current spread of Monkeypox virus

Wastewater based epidemiology beyond SARS-CoV-2: Spanish wastewater reveals the current spread of Monkeypox virus

First Detection of Monkeypox Virus Genome in Sewersheds in France: The Potential of Wastewater-Based Epidemiology for Monitoring Emerging Disease

The basic reproductive ratio of the 2022 outbreak of the monkey pox virus disease for the United Kingdom, Canada, Brazil, the United Arab Emirates, and Nigeria

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