The Detection of Monkeypox Virus DNA in Wastewater Samples in the Netherlands.

Jonge, Eline F. de; Peterse, Céline; Koelewijn, Jaap; Drift, Anne-Merel van der; Beek, Rudolf van der; Nagelkerke, Erwin; Lodder, Willemijn J.

doi:10.2139/ssrn.4168974

Cited by 1 publication

(2 citation statements)

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“…They used the G2R_G assay developed by the CDC (Li et al, 2010) that we optimized in the present study. In Europe, de Jonge and collaborators used the same G2R assay to detect MPXV DNA in urban wastewater samples collected in the Netherlands and, occasionally, from the Amsterdam Schiphol Airport, a major travel hub (de Jonge et al, 2022). Furthermore, the Indian COVID-19 Technical Advisory Committee has recommended wastewater surveillance for early detection of Monkeypox, suggesting beginning with samples from the International Airport, the entry point of international arrivals (TAC recommends sewage surveillance for early detection of Monkeypox in State - The Hindu).…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, viral shedding DNA from saliva, feces, urine, semen, and skin lesions has been demonstrated (Peiró-Mestres et al, 2022; Lapa et al, 2022; Antinori et al, 2022), suggesting that Monkeypox genome is expected to be detected in wastewater. To date, no study in peer-reviewed journal articles has documented the occurrence of Monkeypox in sewage; however, two preprints have been published, one in the Netherlands and one in the United States, in western California (de Jonge et al, 2022; Wolfe et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Monkeypox virus DNA in the wastewater of an airport in Rome, Italy: expanding environmental surveillance to emerging threats

Rosa

Mancini

Veneri

et al. 2022

Preprint

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Since May 2022, monkeypox cases have been reported in non-endemic countries, and the disease was declared a Public Health Emergency of International Concern. Rapid identification of new cases is critical for outbreak control. Environmental surveillance can be used as a complementary tool for detecting pathogens circulating in communities. This study aimed to investigate whether Monkeypox DNA can be detected in wastewater from a transportation hub. Twenty samples were collected in Italy's first airport in Rome and tested using published and modified real-time PCR assays targeting the G2R region (TFN gene), F3L, and N3R genes. Nested PCR assays were also used for confirmation by sequencing. Three samples tested positive by real-time PCR and/or by nested PCR, confirming the occurrence of the virus in the airport's wastewater. Wastewater surveillance can be quickly adapted to investigate emerging threats and can be used to track the introduction and/or the diffusion of the Monkeypox virus in communities.

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Section: Discussionmentioning

confidence: 99%