The Detection of Lumpy Skin Disease Virus Infection by Clinical Findings and PCR Method in Turkey

Abstract

Lumpy Skin Disease (LSD), sığır, bufalo ve bazı yabani türlerin derileri üzerinde nodüller ve ödem ile karakterize viral bir enfeksiyonudur. Bu çalışmada, LSD virus enfeksiyonunun, Türkiye'deki varlığının, enfekte sığırlarda gözlenen klinik bulgulara göre tanısı ve PCR yöntemi ile doğrulanması amaçlanmıştır. Bu amaçla, Eylül 2013-Haziran 2014 tarihleri arasında Türkiye'de yerleşik 8 sığır çiftliğinde klinik olarak LSD enfeksiyonu belirtileri gösteren 113 sığır incelendi. Daha sonra, bu sığırlardan 12 deri nodülü örneği alındı ve viroloji laboratuvarına getirildi. Bu örnekler OIE tarafından tanımlanan yönteme göre PCR ile test edildi. Enfeksiyonun klinik bulgularının PCR sonuçları ile uyumlu olduğu ortaya konuldu. Anahtar sözcükler: Lumpy Skin Disease, PCR, Epidemiyoloji The Detection of Lumpy Skin Disease Virus Infection by Clinical Findings and PCR Method in Turkey AbstractLumpy Skin Disease (LSD) is a viral disease of cattle, buffalo and some wild species characterized by oedema and nodules on the skin. The aim of this study was to report the presence and the clinical signs of LSD in infected cattle in Turkey, and to confirm by PCR method. For this purpose, 113 cattle showing clinical signs of LSD were examined from 8 settled farms in Turkey between September 2013 to June 2014. Then, 12 skin nodule samples from these cattle were obtained, and brought to the virology laboratory. The samples were tested by PCR according to the method described by OIE. The clinical diagnosis was well correlated with PCR results.

Seroprevalence of Maedi-Visna Infection in Sheep in the Central Black Sea Region of Türkiye

“…The first investigation into the virus's presence in Turkey was conducted by Alibaşoğlu et al (1975), focusing on pathological findings for virus detection. Subsequently, many studies have been undertaken to detect the virus using serological and virological methods (Girgin et al, 1987;Karaoğlu et al, 2003;Çimtay et al, 2004;Arslan et al, 2012;Gürçay et al, 2013).…”

A lumpy skin disease case in the southeast turkey: A threat for Eurasia

“…In another study, PCR was also used for diagnosis of LSD in Turkey (Gürçay et al, 2015), authors tested 12 biopsy specimens taken from different cases against LSD virus. They found positive amplification in the all specimens.…”

Nodular Dermatitis: Emergence of Novel Poxviral Infection in Russia

“…диагноз «нодулярный дерматит» считают установленным при выявлении в пробах, полученных от больных или подозреваемых на заболевание животных, днк вируса нодулярного дерматита крс. данные, полученные методом пцр, коррелируют с данными клинической диагностики [1, 9,14]. с помощью пцр-рв удается выявить днк вируса нодулярного дерматита у животных с клиническими признаками заболевания в 100 % случаев [25].…”