The detection of Escherichia coli (E. coli) with the pH sensitive hydrogel nanofiber-light addressable potentiometric sensor (NF-LAPS)

Image detection of yeast Saccharomyces cerevisiae by light-addressable potentiometric sensors (LAPS)

“…Photocurrent measurements using 1,8-nonadiyne modified SOS surfaces displayed the expected behaviour for good quality LAPS substrates. LAPS have been developed for the determination of the extracellular acidification of Escherichia coli and Saccharomyces cerevisiae using pH sensitive surfaces in past reports [12,14,24]. In this report, we were aiming to detect the properties of cells themselves to obtain a common imaging method, which can be widely Figure 3A(b)).…”

“…LAPS has been used successfully for the electrochemical imaging of specific ions, DNA detection, monitoring of enzymatic reactions, microorganisms, activities and extracellular potentials of cells [10][11][12][13][14][15][16] with a typical resolution of about 10-100 µm using LED arrays as the light source. Using an optimized laser scanning setup in conjunction with a silicon-onsapphire (SOS) substrate (Figure 1), 1.5 µm spatial resolution was obtained using visible light with a wavelength of 405 nm, and 0.8 µm resolution was achieved using light with energy smaller than the bandgap exciting a two-photon effect in silicon [17,18].…”

Image Detection of Yeast Saccharomyces Cerevisiae By Light-Addressable Potentiometric Sensors (LAPS)

“…Photocurrent measurements using 1,8-nonadiyne modified SOS surfaces displayed the expected behaviour for good quality LAPS substrates. LAPS have been developed for the determination of the extracellular acidification of Escherichia coli and Saccharomyces cerevisiae using pH sensitive surfaces in past reports [12,14,24]. In this report, we were aiming to detect the properties of cells themselves to obtain a common imaging method, which can be widely used for other cell types in the future.…”

The Detection Method ofEscherichia coliin Water Resources: A Review

“…The feasibility of detecting E. coli in water with simple flow injection are analyse using Chronopotentiometric Aptasensing by J. H. Lei et al Aptamer is used as a bioreceptor and the polyion-sensitive membrane electrode as a signal transducer [55]. Recently, Shaibani et al developed a rapid, simple and low cost E. coli detection with the pH sensitive hydrogel nanofiber-light addressable potentiometric sensor (NF-LAPS) as shown in Figure 6 [56]. The system of NF-LAPS provides a theoretical limit of detection (LOD) of 20 CFU/ml show it very high sensitivity.…”