The detection of cytoplasmic immunoglobulins by immunofluorescence: Improvements in techniques and standardization procedures

Vossen, Jaak M.; Bergh, R Langlois Van Den

doi:10.1016/0022-1759(76)90188-5

Cited by 9 publications

(2 citation statements)

References 12 publications

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“…B one m a rro w sp ecim en s w ere a n a ly z e d fo r plasm a cells by cytoplasm ic im m unofluorescence fo r IgM , IgG , IgA, and IgD (18). A lso, com bined staining for th e presen ce o f surface IgM (slgM ) and slgD on sm all cytoplasm ic IgM (clgM )-positive cells, on large clgM -positive, and o n large clgDpositive cells w as perform ed.…”

Section: Methodsmentioning

confidence: 99%

Clinical and immunological studies in patients with an increased serum IgD level

I¹,

Vossen²,

Meer³

et al. 1989

J Clin Immunol

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Section: Methodsmentioning

confidence: 99%

Clinical and immunological studies in patients with an increased serum IgD level

I¹,

Vossen²,

Meer³

et al. 1989

J Clin Immunol

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“…Detection of cytoplasmic immunoglobulins (clg). Cytocentrifuged mononuclear eell preparations on microscope slides were fixed in methanol for 5 min, washed, and then stained with polyvalent goat antihuman fluorescein-conjugated F(ab')2 fragments (Kallestad Laboratories) [5,30].…”

Section: Methodsmentioning

confidence: 99%

T and B Lymphocytes in Patients with Nickel Sensitivity

1985

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Peripheral blood mononuclear cells from altogether 45 nickel-sensitive patients and 37 controls were assayed for various T and B cell variables. All the patients, but none of the controls, fulfilled our in vitro criteria for a positive response to nickel sulphate (NiSO4). We report normal T and B lymphocyte counts, normal spontaneous plaque-forming cell (PFC) numbers, normal serum immunoglobulin levels, and no demonstrable specific cytotoxic T lymphocyte activity associated with nickel sensitivity. We could detect only a slight increase in the number of PFC and in the number of cytoplasmic immunoglobulin positive (cIg+) cells following stimulation of the patients' cells with NiSO4 for 6 days in culture. Apart from a transient increase in the [3H]thymidine uptake by patients' cells stimulated with NiSO4 in vitro, and a transient drop in the OKT4/OKT8 ratio, there were no major differences in the values of the above variables before and after in vivo challenge of 3 patients with NiSO4. Blood from only 2 of the latter 3 patients was tested in the DNA synthesis test. We conclude that apart from the DNA synthesis test, none of these tests is of any use as an aid to diagnosis in patients with nickel sensitivity. A careful attitude towards patch testing should be maintained.

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A simultaneous comparison of the cells of blood, renal hilar and thoracic duct lymph in the dog

1980

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A simultaneous morphological and quantitative profile was obtained of the cells of blood, thoracic duct, and renal hilar lymph in the dog. Monolayer cytocentrifuged preparations were used to determine the number, type, and size of cells in the three compartments. The cell count of renal lymph was not related to that of blood or thoracic duct lymph. There was a greater percentage of lymphoid cells in the afferent lymph than could be accounted for by the random movement of cells from the blood to the lymph. Thus, there appeared to be a selective transit of cells from blood to lymph. Monocytes and neutrophils were largely absent from the thoracic duct lymph; however, eosinophils were present. Cells were observed in hilar lymph that were characteristic of cells subjected to antigenic stimulation. It was concluded that lymphocytes have a preferential pathway from blood to lymphatic and in the course of this pathway they undergo a change which is consistent with an active immunological role.

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The detection of cytoplasmic immunoglobulins by immunofluorescence: Improvements in techniques and standardization procedures

Cited by 9 publications

References 12 publications

Clinical and immunological studies in patients with an increased serum IgD level

Clinical and immunological studies in patients with an increased serum IgD level

T and B Lymphocytes in Patients with Nickel Sensitivity

A simultaneous comparison of the cells of blood, renal hilar and thoracic duct lymph in the dog

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