The detection of antibodies to mycobacterium tuberculosis by microplate enzyme-linked immunosorbent assay (ELISA)

Nassau, E.; Parsons, Ella; Johnson, Glynis

doi:10.1016/0041-3879(76)90019-2

Cited by 114 publications

(35 citation statements)

References 3 publications

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“…In the recent years, proteins that are secreted by mycobacteria have gained importance due to the high host immuno response against these proteins and further they are secreted by live bacilli thus indicator of active infection. There have been reports on the usefulness of culture filtrate antigens in the diagnosis of tuberculosis by ELISA method (5,6,20,21). In the earlier studies from our laboratory we have reported the potential of Mycobacterium tuberculosis H37Ra excretory secretory (Mtb ES) antigen in the diagnosis of tuberculosis (15122,23).…”

Section: Discussionmentioning

confidence: 98%

“…These limitations make it imperative that more sensitive and specific serologic tests be developed for tuberculosis. Since the first description of Enzyme Linked Immunosorbent Assay (ELISA) for tuberculosis by Nassau et al (5), the assay has been widely tried in serodiagnosis, using various myocobacterial preparations ranging from crude extracts to purified antigens.…”

Section: Introductionmentioning

confidence: 99%

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Diagnostic potential of fractionatedMycobacterium tuberculosis H37Ra excretory-secretory (EST-DE1) antigen in pulmonary tuberculosis

Lodam

Pramanik

Reddy

et al. 1997

Indian J Clin Biochem

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Tuberculosis is emerging as a major public health problem in developing and developed wodd. Early and precise diagnosis is of prime importance in successful control of Infection. Indirect ELISA with penicillinase as marker was developed using purified M. tuberculosis excretory-secretory (EST-DEt) antigen for detecting IgG antibodies in pulmonary tuberculosis. The assay System gave a overall sensitivity of 82% for both smear positive and smear negative pulmonary tuberculosis cases with a specificity of 84%. The positive and negative predictive values were 75% and 88% respectivaly. Further studies with EST-DE t antigen revealed that, it contains two of the active antigen fractions of Mtb EST antigen i.e. Mtb and Mtb , as demonstrated by inhibition ELISA. Reactivity with monoclonal antibodies HGT 3a showed the presence of 38 KDa molecule in EST-DE 1 antigen.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Diagnostic potential of fractionatedMycobacterium tuberculosis H37Ra excretory-secretory (EST-DE1) antigen in pulmonary tuberculosis

Lodam

Pramanik

Reddy

et al. 1997

Indian J Clin Biochem

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“…Several antigens have been evaluated for their serodiagnostic potential in detecting circulating antibodies by enzyme-linked immunosorbent assay (ELISA) (Nassau et al, 1976;Grange et al, 1980;Reggiardo et al, 1980;Daniel et al, 1981;Zeiss et al, 1982;Benjamin and Daniel, 1982;Stroebel et al, 1982;Benjamin et al, 1984). The sensitivities and specificities of these assays vary considerably and interpretation of the reported results is difficult because of limited clinical information.…”

Section: Introductionmentioning

confidence: 99%

Detection of antibodies to Mycobacterium tuberculosis plasma membrane antigen by enzyme-linked immunosorbent assay

Krambovitis

1986

Journal of Medical Microbiology

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“…The method used for the ELJSA has been described (Cromie, 1991) and is based on the methods of Nassau et al (1976) and Bahr et al (1980). The antigens were prepared by the method of Stanford et al (1975) using M. fortuitum, M. vaccae and two strains of M. avium originally isolated from tuberculous birds from Slimbridge.…”

Section: Elisamentioning

confidence: 99%

A comparison and evaluation of techniques for diagnosis of avian tuberculosis in wildfowl

et al. 1993

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SUMMARYTo control the epizootic of avian tuberculosis within the collections of captive wildfowl of The Wildfowl and Wetlands Trust an efficacious vaccine and a reliable diagnostic test are required.A number of potential diagnostic tests were compared for sensitivity and specificity using a flock of 178 feral barnacle geese (Branta leucopsis) at The Wildfowl and Wetland Trust's centre at Slimbridge, Gloucestershire. Evaluations were made of: serodiagnosis by an enzyme-linked immunosorbent assay (ELISA); agglutination of a suspension of killed Mycobacterium avium using both whole blood and serum; and haematological analysis.Necropsy findings confirmed the ELISA to be sensitive and specific, and capable of detecting the disease even at an early stage. The agglutination tests were quick and easy to perform although a number of false positives and negatives did occur. The haematological analysis was found to be less sensitive. ELISA and agglutination tests are now being used to screen the birds in the collections.

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The detection of antibodies to mycobacterium tuberculosis by microplate enzyme-linked immunosorbent assay (ELISA)

Cited by 114 publications

References 3 publications

Diagnostic potential of fractionatedMycobacterium tuberculosis H37Ra excretory-secretory (EST-DE1) antigen in pulmonary tuberculosis

Diagnostic potential of fractionatedMycobacterium tuberculosis H37Ra excretory-secretory (EST-DE1) antigen in pulmonary tuberculosis

Detection of antibodies to Mycobacterium tuberculosis plasma membrane antigen by enzyme-linked immunosorbent assay

A comparison and evaluation of techniques for diagnosis of avian tuberculosis in wildfowl

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