The detection and measurement of circulating gastrin-like activity by bioassay

The Bayliss‐Starling lecture 1973. The gastrointestinal hormones: a review of recent advances.

“…There is undoubtedly more to be unearthed in this corner of the gastrin field. For instance, evidence has recently been obtained which indicates that in duodenal ulcer patients the value for circulating gastric measured by direct bioassay of serum substantially exceeds that obtained by radioimmunoassay using an antibody raised to the heptadecapeptide, but not when the antibody has been raised to the much smaller pentagastrim molecule (Herring &;Blair, 1969;Colin-Jones &;Lennard-Jones, 1972;24 B. A. GREGORY GASTROINTESTINAL HORMONES Byrnes, Coleman & Lazarus, 1971); this suggests that there may be in circulation active C-terminal gastrin fragments too small to react significantly with the heptadecapeptide antibody.…”