The detection and estimation of <i>Verticillium dahliae</i> in naturally infested soil

Harris, D. C.; Yang, Jiarong; Ridout, Martin S.

doi:10.1111/j.1365-3059.1993.tb01496.x

Cited by 74 publications

(54 citation statements)

References 32 publications

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“…For instance, it is advisable to avoid fields in close proximity with V. dahliae-susceptible crops, or to avoid soils where susceptible hosts are known to have been previously cropped. But most importantly, it is highly recommended to assess the presence of pathogen propagules in soil, either by traditional microbiological isolation procedures (Harris et al 1993;López-Escudero and Blanco-López 2001), or by molecular detection assays (Pérez-Artés et al 2005).…”

Section: Use Of Healthy Soilsmentioning

confidence: 99%

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Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

2010

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Olive (Olea europaea L.) is one of the first domesticated and cultivated tree species and has historical, social and economical relevance. However, its future as a strategic commodity in Mediterranean agriculture is threatened by diverse biotic (traditional and new/emerging pests and diseases) and abiotic (erosion, climate change) menaces. These problems could also be of relevance for new geographical areas where olive cultivation is not traditional but is increasingly spreading (i.e., South America, Australia, etc). One of the major constraints for olive cultivation is Verticillium wilt, a vascular disease caused by the soil-borne fungus Verticillium dahliae Kleb. In this review we describe how Verticillium wilt of olive (VWO) has become a major problem for olive cultivation during the last two decades. Similar to other vascular diseases, VWO is difficult to manage and single control measure are mostly ineffective. Therefore, an integrated disease management strategy that fits modern sustainable agriculture criteria must be implemented. Multidisciplinary research efforts and advances to understand this pathosystem and to develop appropriate control measures are summarized. The main conclusion is that a holistic approach is the best strategy to effectively control VWO, integrating biological, chemical, physical, and cultural approaches.

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Section: Use Of Healthy Soilsmentioning

confidence: 99%

“…Traditional microbiological methods used to quantify V. dahliae MS in soil has been thoroughly reviewed by Harris et al (1993). These techniques involve the plating of a defined amount of soil, previously processed by diverse methods (i.e.…”

Section: A) Estimation Of Verticillium Dahliae Inoculum Potential In mentioning

confidence: 99%

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

2010

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“…The plants were placed in a shifted double row (0.25 × 0.5 m). The soil was highly infested with V. dahliae (15.8 microsclerotia/g soil) which was determined by the wet-sieving method (Harris et al 1993).…”

Section: Methodsmentioning

confidence: 99%

New strawberry genotypes tested for organic production on a Verticillium-infested site

Weissinger¹,

Flachowsky²,

Spornberger³

2014

Hort. Sci. (Prague)

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Weissinger H., Flachowsky H., Spornberger A., 2014. New strawberry genotypes tested for organic production on a Verticillium-infested site. Hort. Sci. (Prague), 41: 167-174.Nineteen new strawberry breeding clones, bred at the Institute for Breeding Research on Fruit Crops in Dresden-Pillnitz, Germany, were evaluated for their suitability for organic production at a Verticillium-infested site in Vienna, Austria. Soil cover, plant vitality, resistance to leaf spot diseases as well as flower damages by the strawberry blossom weevil (Anthonomus rubi) and by spring frost were recorded. In two consecutive years, marketable yield and fruits infested by Botrytis cinerea were assessed. Three breeding clones, P-7189, P-8043 and P-8071, were considered as suitable for organic strawberry production. P-7189 and P-8043 showed high tolerance to Verticillium wilt. The breeding clones P-8155 and P-8166 performed well under organic management but had very soft fruits that were not acceptable for commercial fruit production. The rest of the breeding clones were low-yielding, however, some of them could be of interest for home gardens due to their good flavour and high plant vitality.

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“…The content of the bag filled with V. dahliae inoculum microsclerotia were ground, and 0.003 g of the material was brought onto a specific Verticillium medium (Modified soil extract agar (MSEA) (Harris et al, 1993) in threefold. Germinating microsclerotia were scored.…”

Section: Methodsmentioning

confidence: 99%

Biological Soil Disinfestation With Organic Fermentation Products

Ludeking¹,

Paternotte²,

Runia³

et al. 2011

Acta Hortic.

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Much research has been done on biological disinfestation of soil with grass and other fresh organic materials to suppress persistent diseases like Verticillium dahliae and nematode populations in the soil. After covering the soil with airtight plastic and creating an anaerobic environment, natural processes that will have a disinfesting effect on the soil are stimulated by the organic material. Practical conditions may vary enormously and so it seems that soil disinfestation with grass or other fresh materials is unpredictable. Growers find this method complicated and labor intensive. In particular, growers of horticultural crops are not enthusiastic about biological soil disinfestation with grass. As an alternative for fresh materials, organic fermentation products have been tested.Application is easier and effects occur faster than with fresh organic materials. Therefore, this method offers a chance to explore the possibilities for broad application in horticulture as an alternative for soil disinfestation with steam. Product H7022 (ThatchTec B.V., Wageningen, The Netherlands), consists of organic by-products from the food processing industry, and resulted in a 100% reduction of nematodes and Verticillium dahliae. The major drawback to this method is the treatment time of at least two weeks, which is too long for most greenhouse growers, as the costs of a two week time gap in production are too high, making it difficult to adapt to a greenhouse system. New research on organic fermentation of the soil is necessary to determine which processes cause soil disinfestation, during what time period this method causes an effect, and whether or not this method is suitable for use in soil-bound horticulture.

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The detection and estimation of Verticillium dahliae in naturally infested soil

Cited by 74 publications

References 32 publications

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

New strawberry genotypes tested for organic production on a Verticillium-infested site

Biological Soil Disinfestation With Organic Fermentation Products

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