The detection and characterisation of Dichelobacter nodosus from cases of ovine footrot in England and Wales

Moore, Lindsey; Wassink, G. J.; Green, Laura; Grogono-Thomas, Rosemary

doi:10.1016/j.vetmic.2005.01.029

Cited by 83 publications

(102 citation statements)

References 27 publications

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“…were detected in only one sheep with ID from flock C. There were two sequences and they had 94 and 99% similarity to uncultured Treponema phylotypes from samples from cattle with digital dermatitis and from animal faecal samples (Klitgaard et al, 2008;Ley et al, 2008). Treponema are often free living but are linked to contagious ovine digital dermatitis and bovine digital dermatitis in sheep and cattle, respectively (Collighan et al, 2000;Moore et al, 2005;Demirkan et al, 2006;Evans et al, 2008Evans et al, , 2009Sayers et al, 2009). Several species have been associated to contagious ovine digital dermatitis and DD including Treponema phagedenis-like and Treponema medium/Treponema vincentii-like, Treponema medium/Treponema vincentii-like, Treponema phagedenis-like, and Treponema denticola/ Treponema putidum-like (Sayers et al, 2009).…”

Section: Comparison Of the Microbial Communities And Taxonomic Classimentioning

confidence: 99%

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Ovine pedomics: the first study of the ovine foot 16S rRNA-based microbiome

et al. 2011

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We report the first study of the bacterial microbiome of ovine interdigital skin based on 16S rRNA by pyrosequencing and conventional cloning with Sanger-sequencing. Three flocks were selected, one a flock with no signs of footrot or interdigital dermatitis, a second flock with interdigital dermatitis alone and a third flock with both interdigital dermatitis and footrot. The sheep were classified as having either healthy interdigital skin (H) and interdigital dermatitis (ID) or virulent footrot (VFR). The ovine interdigital skin bacterial community varied significantly by flock and clinical condition. The diversity and richness of operational taxonomic units was greater in tissue from sheep with ID than H or VFR-affected sheep. Actinobacteria, Bacteriodetes, Firmicutes and Proteobacteria were the most abundant phyla comprising 25 genera. Peptostreptococcus, Corynebacterium and Staphylococcus were associated with H, ID and VFR, respectively. Sequences of Dichelobacter nodosus, the causal agent of ovine footrot, were not amplified because of mismatches in the 16S rRNA universal forward primer (27F). A specific real-time PCR assay was used to demonstrate the presence of D. nodosus, which was detected in all samples including the flock with no signs of ID or VFR. Sheep with ID had significantly higher numbers of D. nodosus (10 4 -10 9 cells per g tissue) than those with H or VFR feet.

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Section: Comparison Of the Microbial Communities And Taxonomic Classimentioning

confidence: 99%

“…and Clostridium spp. among others (Berveridge 1941;Moore et al, 2005). Cultivation of bacteria from affected goats showed that the major taxa were D. nodosus, Peptostreptococcus, Megasphaera and Fusobacterium (Piriz Duran et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Ovine pedomics: the first study of the ovine foot 16S rRNA-based microbiome

et al. 2011

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“…Moreover, the gelatin-gel and elastase assays to test for protease activity, traditionally carried out to detect and differentiate virulent and benign D. nodosus strains, is difficult to perform and is strongly dependent on the quality of the growth medium and hence may yield inconsistent results (4). PCR-based approaches have markedly improved sensitivity and speed of detection of D. nodosus (3,(5)(6)(7)(8)(9). However, a method of fast, reliable and, ideally, simultaneous detection and virulotyping does to date not exist.…”

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confidence: 99%

Simultaneous Detection and Discrimination of Virulent and Benign Dichelobacter nodosus in Sheep of Flocks Affected by Foot Rot and in Clinically Healthy Flocks by Competitive Real-Time PCR

et al. 2014

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Ovine foot rot caused by Dichelobacter nodosus is affecting sheep worldwide. The current diagnostic methods are difficult and cumbersome. Here, we present a competitive real-time PCR based on allelic discrimination of the protease genes aprV2 and aprB2. This method allows direct detection and differentiation of virulent and benign D. nodosus from interdigital skin swabs in a single test. Clinically affected sheep harbored high loads of only virulent strains, whereas healthy sheep had lower loads of predominantly benign strains.

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“…Quanto ao exame microbiológico a porcentagem de isolamento neste trabalho (62%) é semelhante ao de Jimenez et al (2003) que isolou D. nodosus em 64,5% das 45 amostras provenientes da região do Alentejo em Portugal, Hussain et al (2009) com 64% das 128 amostras na Ín-dia; próxima aos 56,8% obtidos por Piriz et al (1990) em um estudo na região de Caceres na Espanha e Moore et al (2005) com 56% das 95 amostras de ovinos com pododermatite na Inglaterra e Pais de Gales. Por outro lado, o isolamento foi inferior ao publicado por Hurtado et al (1998) que isolou 77,7% em um estudo com 216 amostras de ovinos com pododermatite na Espanha.…”

Section: Discussionunclassified

Evolução clínica e avaliação de parâmetros leucocitários e de proteínas de fase aguda na pododermatite infecciosa ovina

Carvalho

Araújo²,

Vasconcelos³

et al. 2012

Pesq. Vet. Bras.

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ABSTRACT.-Carvalho V.S., Araújo B.R., Vasconcelos T.C., Chiminazzo C., Costa Neto A.O., Ayres M.C.C., Guimarães J. In this paper, Santa Inês crossbred sheep with footrot were evaluated, checking the changes on leukogram and the acute phase proteins. In the ϐirst experiment, 70 females were divided into three groups according to their podal scores: a control group (G1) with score 0, scald group (G2) with score 1 or 2 and a footrot group (G3) with score 3, 4 or 5. During two months, the clinical course and its effect on leukogram were observed at ϐive moments: M1 (day 0), M2 (day 15), M3 (day 30), M4 (day 45) and M5 (day 60). The intensity of the white blood cell count was low magnitude, observing signiϐicant changes (p <0.05) as a mild leukocytosis (G3, M4), attributed to neutrophilia and a slight increase in the total number of monocytes (G3, M2 and M3) despite the extensive damage and tissue necrosis existed during the ϐinal stage of the disease. In the second experiment, 105 animals from seven farms were used with the objective of isolating the causative agent and to evaluate the effect of disease on total plasma protein and acute phase proteins. The animals were also divided into three groups: control, scald and footrot. In all farms, the isolation of Dichelobacter nodosus was regarded. For the different stages of the disease on the proteins studied there was no signiϐicant correlation (p<0.05) but the haptoglobin in scald and footrot groups showed higher average than the control group. Based on these results, we conclude that the acute phase proteins studied were not efϐicient in the characterization of the phases of the disease and the leukocyte response was mild, making it difϐicult to use for this purpose.

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The detection and characterisation of Dichelobacter nodosus from cases of ovine footrot in England and Wales

Cited by 83 publications

References 27 publications

Ovine pedomics: the first study of the ovine foot 16S rRNA-based microbiome

Ovine pedomics: the first study of the ovine foot 16S rRNA-based microbiome

Simultaneous Detection and Discrimination of Virulent and Benign Dichelobacter nodosus in Sheep of Flocks Affected by Foot Rot and in Clinically Healthy Flocks by Competitive Real-Time PCR

Evolução clínica e avaliação de parâmetros leucocitários e de proteínas de fase aguda na pododermatite infecciosa ovina

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