The Design, Synthesis, and Activity of a Ribozyme That Specially Cleaves Rat 28S rRNA

The Design and Construction of a New Nucleozyme

“…First, the copolymer must be stable in vivo and in vitro; second, the replication must be a template‐directed reaction; third, it must contain an initiator, a terminator, and an Ori sequence as a replication unit. Based on the above considerations, we constructed a cycle nucleozyme molecule that was composed of T7 promoter, RI gene, 4 and RII. 5 It was transformed into E. coli JM101 cells and its duplexes were obtained from the host cells.…”