The design of strut/TPMS-based pore geometries in bioceramic scaffolds guiding osteogenesis and angiogenesis in bone regeneration

Li, Yifan; Li, Jiafeng; Jiang, Shuai; Zhong, Cheng; Zhao, Chenchen; Jiao, Yang; Shen, Jian; Chen, Huaizhi; Ye, Meihan; Zhou, Jiayu; Yang, Xianyan; Gou, Zhongru; Xu, Sanzhong; Shen, Ming-Chang

doi:10.1016/j.mtbio.2023.100667

Cited by 15 publications

(17 citation statements)

References 61 publications

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“…Scaffolds with hexagonal-shaped pores demonstrated angiogenesis at a significantly slower rate than the scaffolds with gyroid-shaped pores [ 20 ]. This finding is consistent with more recent studies, which showed enhanced HUVEC migration and tube formation in gyroid scaffolds compared to cubic and cylindrical pore scaffolds [ 21 ].…”

Section: Discussionsupporting

confidence: 93%

“…Corroborating these results, several studies have reported the angiogenic properties of the gyroid structure, noting that pore size and interconnectivity have essential roles in blood vessels [ 19 , 20 , 21 ]. Scaffolds with a 200–400 μm pore diameter demonstrated the formation of a large blood vascular network of vessels with deep penetration depth [ 20 ].…”

Section: Discussionmentioning

confidence: 85%

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In Vivo Osteogenic and Angiogenic Properties of a 3D-Printed Isosorbide-Based Gyroid Scaffold Manufactured via Digital Light Processing

Verisqa,

Park,

Mandakhbayar

et al. 2024

Biomedicines

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Introduction: Osteogenic and angiogenic properties of synthetic bone grafts play a crucial role in the restoration of bone defects. Angiogenesis is recognised for its support in bone regeneration, particularly in larger defects. The objective of this study is to evaluate the new bone formation and neovascularisation of a 3D-printed isosorbide-based novel CSMA-2 polymer in biomimetic gyroid structures. Methods: The gyroid scaffolds were fabricated by 3D printing CSMA-2 polymers with different hydroxyapatite (HA) filler concentrations using the digital light processing (DLP) method. A small animal subcutaneous model and a rat calvaria critical-size defect model were performed to analyse tissue compatibility, angiogenesis, and new bone formation. Results: The in vivo results showed good biocompatibility of the 3D-printed gyroid scaffolds with no visible prolonged inflammatory reaction. Blood vessels were found to infiltrate the pores from day 7 of the implantation. New bone formation was confirmed with positive MT staining and BMP-2 expression, particularly on scaffolds with 10% HA. Bone volume was significantly higher in the CSMA-2 10HA group compared to the sham control group. Discussion and Conclusions: The results of the subcutaneous model demonstrated a favourable tissue response, including angiogenesis and fibrous tissue, indicative of the early wound healing process. The results from the critical-size defect model showcased new bone formation, as confirmed by micro-CT imaging and immunohistochemistry. The combination of CSMA-2 as the 3D printing material and the gyroid as the 3D structure was found to support essential events in bone healing, specifically angiogenesis and osteogenesis.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 85%

In Vivo Osteogenic and Angiogenic Properties of a 3D-Printed Isosorbide-Based Gyroid Scaffold Manufactured via Digital Light Processing

Verisqa,

Park,

Mandakhbayar

et al. 2024

Biomedicines

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“…Furthermore, there is evidence that cubic scaffolds enable better cell survival, possibly due to the 3D mesh structure that allows nutrients, oxygen, and metabolites to circulate more easily inside the scaffold [46,69,97]. This 3D structure also provides a larger contact area that is more conducive to cell attachment, proliferation, and migration [50,98].…”

Section: Scaffold Shapementioning

confidence: 99%

3D bioprinting approaches for spinal cord injury repair

Jiu,

Liu,

et al. 2024

Biofabrication

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Regenerative healing of spinal cord injury poses an ongoing medical challenge by causing persistent neurological impairment and a significant socioeconomic burden. The complexity of spinal cord tissue presents hurdles to successful regeneration following injury, due to the difficulty of forming a biomimetic structure that faithfully replicates native tissue using conventional tissue engineering scaffolds. 3D bioprinting is a rapidly evolving technology with unmatched potential to create 3D biological tissues with complicated and hierarchical structure and composition. With the addition of biological additives such as cells and biomolecules, 3D bioprinting can fabricate preclinical implants, tissue or organ-like constructs, and in vitro models through precise control over the deposition of biomaterials and other building blocks. This review highlights the characteristics and advantages of 3D bioprinting for scaffold fabrication to enable spinal cord injury repair, including bottom-up manufacturing, mechanical customization, and spatial heterogeneity. This review also critically discusses the impact of various fabrication parameters on the efficacy of spinal cord repair using 3D bioprinted scaffolds, including the choice of printing method, scaffold shape, biomaterials, and biological supplements such as cells and growth factors. High-quality preclinical studies are required to accelerate the translation of 3D bioprinting into clinical practice for spinal cord repair. Meanwhile, other technological advances will continue to improve the regenerative capability of bioprinted scaffolds, such as the incorporation of nanoscale biological particles and the development of 4D printing.

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“…The scaffolds exhibited strong mechanical strength and non-cytotoxicity. Li et al [117] prepared Mg/Sr co-doped silicate bioceramic (MS-CSi) scaffolds with three different pore geometries (gyroid, cylindrical, and cubic shapes) and compared their effects on osteogenesis and angiogenesis in vitro and in vivo.…”

Section: Bioceramicsmentioning

confidence: 99%

Advancements in gradient bone scaffolds: enhancing bone regeneration in the treatment of various bone disorders

Zhen,

Shi,

Wang

et al. 2024

Biofabrication

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Bone scaffolds are widely employed for treating various bone disorders, including defects, fractures, and accidents. Gradient bone scaffolds present a promising approach by incorporating gradients in shape, porosity, density, and other properties, mimicking the natural human body structure. This design offers several advantages over traditional scaffolds. A key advantage is the enhanced matching of human tissue properties, facilitating cell adhesion and migration. Furthermore, the gradient structure fosters a smooth transition between scaffold and surrounding tissue, minimizing the risk of inflammation or rejection. Mechanical stability is also improved, providing better support for bone regeneration. Additionally, gradient bone scaffolds can integrate drug delivery systems, enabling controlled release of drugs or growth factors to promote specific cellular activities during the healing process. This comprehensive review examines the design aspects of gradient bone scaffolds, encompassing structure and drug delivery capabilities. By optimizing the scaffold's inherent advantages through gradient design, bone regeneration outcomes can be improved. The insights presented in this article contribute to the academic understanding of gradient bone scaffolds and their applications in bone tissue engineering.

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The design of strut/TPMS-based pore geometries in bioceramic scaffolds guiding osteogenesis and angiogenesis in bone regeneration

Cited by 15 publications

References 61 publications

In Vivo Osteogenic and Angiogenic Properties of a 3D-Printed Isosorbide-Based Gyroid Scaffold Manufactured via Digital Light Processing

In Vivo Osteogenic and Angiogenic Properties of a 3D-Printed Isosorbide-Based Gyroid Scaffold Manufactured via Digital Light Processing

3D bioprinting approaches for spinal cord injury repair

Advancements in gradient bone scaffolds: enhancing bone regeneration in the treatment of various bone disorders

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