The Design of Biodegradable Microcarriers for Induced Cell Aggregation

Tan, Huaping; Wu, Jindan; Huang, Dejuan; Gao, Changyou

doi:10.1002/mabi.200900160

Cited by 45 publications

(31 citation statements)

References 36 publications

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“…Constructs containing MCs, cells, and ECM proteins were formed, which were suitable for injection; these chondrocyte–MC aggregates could be delivered, arthroscopically, into the cartilage defect or used for meniscus reconstruction. 33,35 …”

Section: Discussionmentioning

confidence: 99%

Dynamic cell culture on calcium phosphate microcarriers for bone tissue engineering applications

et al. 2014

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Developing appropriate cell culturing techniques to populate scaffolds has become a great challenge in tissue engineering. This work describes the use of spinner flask dynamic cell cultures to populate hydroxyapatite microcarriers for bone tissue engineering. The microcarriers were obtained through the emulsion of a self-setting aqueous α-tricalcium phosphate slurry in oil. After setting, hydroxyapatite microcarriers were obtained. The incorporation of gelatin in the liquid phase of the α-tricalcium phosphate slurry allowed obtaining hybrid gelatin/hydroxyapatite-microcarriers. Initial cell attachment on the microcarriers was strongly influenced by the speed of the dynamic culture, achieving higher attachment at low speed (40 r/min) as compared to high speed (80 r/min). Under moderate culture speeds (40 r/min), the number of cells present in the culture as well as the number of microcarrier-containing cells considerably increased after 3 days, particularly in the gelatin-containing microcarriers. At longer culture times in dynamic culture, hydroxyapatite-containing microcarriers formed aggregates containing viable and extracellular matrix proteins, with a significantly higher number of cells compared to static cultures.

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Section: Discussionmentioning

confidence: 99%

Dynamic cell culture on calcium phosphate microcarriers for bone tissue engineering applications

et al. 2014

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“…Biodegradable microspheres and nanoparticles have been extensively used as drug carriers [85]. Biodegradable polymers such as poly(lactic acid) (PLA), poly(lactide-co-glycolide) (PLGA), chitosan, gelatin and alginate are now largely used to prepare microspheres and nanoparticles [2,69,70,71].…”

Section: Major Systemsmentioning

confidence: 99%

Alginate-Based Biomaterials for Regenerative Medicine Applications

2013

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Alginate is a natural polysaccharide exhibiting excellent biocompatibility and biodegradability, having many different applications in the field of biomedicine. Alginate is readily processable for applicable three-dimensional scaffolding materials such as hydrogels, microspheres, microcapsules, sponges, foams and fibers. Alginate-based biomaterials can be utilized as drug delivery systems and cell carriers for tissue engineering. Alginate can be easily modified via chemical and physical reactions to obtain derivatives having various structures, properties, functions and applications. Tuning the structure and properties such as biodegradability, mechanical strength, gelation property and cell affinity can be achieved through combination with other biomaterials, immobilization of specific ligands such as peptide and sugar molecules, and physical or chemical crosslinking. This review focuses on recent advances in the use of alginate and its derivatives in the field of biomedical applications, including wound healing, cartilage repair, bone regeneration and drug delivery, which have potential in tissue regeneration applications.

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“…Over the past decade, many methods have been employed for preparation of biodegradable hydrogels, which allow easy and homogenous drug distribution within any defect size or shape [4][5][6]. Conventional techniques for hydrogel synthesis are limited by involvement of cytotoxic reagents, instability of the functional groups, possible side reactions and low coupling efficiency [7][8][9][10][11][12]. Recently, a versatility of click chemistry methods has been broadly utilized to prepare biodegradable hydrogels with specific association mechanisms, the most common example being the copper (I)-catalyzed reaction of azides with alkynes [13][14][15].…”

Section: Introductionmentioning

confidence: 99%